Expression Evolution of Mammalian Genes.
dc.contributor.author | Liao, Ben-Yang | en_US |
dc.date.accessioned | 2008-08-25T20:56:02Z | |
dc.date.available | NO_RESTRICTION | en_US |
dc.date.available | 2008-08-25T20:56:02Z | |
dc.date.issued | 2008 | en_US |
dc.date.submitted | en_US | |
dc.identifier.uri | https://hdl.handle.net/2027.42/60816 | |
dc.description.abstract | Comparing the expression-profiles of over 10,000 genes from the human and mouse genomes, I address fundamental questions on mammalian gene expression. First, I demonstrate that over 80% of human-mouse orthologous genes are evolutionarily conserved in their expression-profiles. This result highlights the importance of proper gene expression to fitness. Second, I show that highly expressed and tissue-specific genes tend to evolve slowly in expression-profile, implying that the expression pattern is of particular importance to highly expressed and tissue-specific genes. I then investigate the potential roles that gene expression plays in protein sequence evolution, dynamics of genome organization, and evolutionary changes of gene essentiality in mammals. My results indicate that tissue-specificity is a stronger determinant on protein evolutionary rate than gene expression level, a factor that is known to be the most important rate determinant in yeasts. The result suggests a great variation in rate determinants of protein sequence evolution between unicellular and multicellular organisms. Subsequently, my analyses on the origin of co-expressed gene clusters indicate that co-expression of linked genes is a form of transcriptional interference that is disadvantageous to organisms, suggesting that transcriptional interference may promote recurrent relocations of genes in the genome. Lastly, I study underlying mechanisms of the evolution of gene essentiality. The results show that the changes of gene essentiality appear to be associated with adaptive evolution at the protein-sequence level, while gene duplication and gene expression evolution plays a negligible role. Together, my studies help understand patterns, mechanisms and consequences of gene expression evolution. | en_US |
dc.format.extent | 2687246 bytes | |
dc.format.extent | 1373 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | en_US |
dc.subject | Human | en_US |
dc.subject | Mouse | en_US |
dc.subject | Transcriptome | en_US |
dc.subject | Genome | en_US |
dc.subject | Molecular Evolution | en_US |
dc.subject | Gene Regulation | en_US |
dc.title | Expression Evolution of Mammalian Genes. | en_US |
dc.type | Thesis | en_US |
dc.description.thesisdegreename | PhD | en_US |
dc.description.thesisdegreediscipline | Ecology and Evolutionary Biology | en_US |
dc.description.thesisdegreegrantor | University of Michigan, Horace H. Rackham School of Graduate Studies | en_US |
dc.contributor.committeemember | Zhang, Jianzhi | en_US |
dc.contributor.committeemember | Qin, Zhaohui | en_US |
dc.contributor.committeemember | Tucker, Priscilla K. | en_US |
dc.contributor.committeemember | Wittkopp, Patricia | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/60816/1/liaoby_1.pdf | |
dc.owningcollname | Dissertations and Theses (Ph.D. and Master's) |
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