Bioassembly of three-dimensional embryonic stem cell-scaffold complexes using compressed gases
dc.contributor.author | Xie, Yubing | en_US |
dc.contributor.author | Yang, Yong | en_US |
dc.contributor.author | Kang, Xihai | en_US |
dc.contributor.author | Li, Ruth | en_US |
dc.contributor.author | Volakis, Leonithas I. | en_US |
dc.contributor.author | Zhang, Xulang | en_US |
dc.contributor.author | Lee, Ly James | en_US |
dc.contributor.author | Kniss, Douglas A. | en_US |
dc.date.accessioned | 2009-05-04T18:24:44Z | |
dc.date.available | 2010-04-14T17:40:05Z | en_US |
dc.date.issued | 2009-03 | en_US |
dc.identifier.citation | Xie, Yubing; Yang, Yong; Kang, Xihai; Li, Ruth; Volakis, Leonithas I.; Zhang, Xulang; Lee, L. James; Kniss, Douglas A. (2009). "Bioassembly of three-dimensional embryonic stem cell-scaffold complexes using compressed gases." Biotechnology Progress 25(2): 535-542. <http://hdl.handle.net/2027.42/62125> | en_US |
dc.identifier.issn | 8756-7938 | en_US |
dc.identifier.issn | 1520-6033 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/62125 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19334083&dopt=citation | en_US |
dc.description.abstract | Tissues are composed of multiple cell types in a well-organized three-dimensional (3D) microenvironment. To faithfully mimic the tissue in vivo , tissue-engineered constructs should have well-defined 3D chemical and spatial control over cell behavior to recapitulate developmental processes in tissue- and organ-specific differentiation and morphogenesis. It is a challenge to build a 3D complex from two-dimensional (2D) patterned structures with the presence of cells. In this study, embryonic stem (ES) cells grown on polymeric scaffolds with well-defined microstructure were constructed into a multilayer cell-scaffold complex using low pressure carbon dioxide (CO 2 ) and nitrogen (N 2 ). The mouse ES cells in the assembled constructs were viable, retained the ES cell-specific gene expression of Oct-4, and maintained the formation of embryoid bodies (EBs). In particular, cell viability was increased from 80% to 90% when CO 2 was replaced with N 2 . The compressed gas-assisted bioassembly of stem cell-polymer constructs opens up a new avenue for tissue engineering and cell therapy. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009 | en_US |
dc.format.extent | 243874 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Biotechnology | en_US |
dc.title | Bioassembly of three-dimensional embryonic stem cell-scaffold complexes using compressed gases | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Dept. of Mechanical Engineering, The University of Michigan, Ann Arbor, MI | en_US |
dc.contributor.affiliationother | College of Nanoscale Science and Engineering, University of Albany, Albany, NY, and Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices (NSEC-CANPBD), The Ohio State University, Columbus, OH | en_US |
dc.contributor.affiliationother | Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices (NSEC-CANPBD), The Ohio State University, Columbus, OH ; Dept. of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210 | en_US |
dc.contributor.affiliationother | Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices (NSEC-CANPBD), The Ohio State University, Columbus, OH | en_US |
dc.contributor.affiliationother | Integrated Biomedical Science Graduate Program, School of Biomedical Science, College of Medicine, The Ohio State University, Columbus, OH ; Laboratory of Perinatal Research, Dept. of Obstetrics and Gynecology, The Ohio State University, Columbus, OH | en_US |
dc.contributor.affiliationother | Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices (NSEC-CANPBD), The Ohio State University, Columbus, OH | en_US |
dc.contributor.affiliationother | Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices (NSEC-CANPBD), The Ohio State University, Columbus, OH ; Dept. of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210 ; Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices (NSEC-CANPBD), The Ohio State University, Columbus, OH | en_US |
dc.contributor.affiliationother | Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices (NSEC-CANPBD), The Ohio State University, Columbus, OH ; Laboratory of Perinatal Research, Dept. of Obstetrics and Gynecology, The Ohio State University, Columbus, OH ; Dept. of Biomedical Engineering, The Ohio State University, Columbus, OH ; Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices (NSEC-CANPBD), The Ohio State University, Columbus, OH | en_US |
dc.identifier.pmid | 19334083 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/62125/1/151_ftp.pdf | |
dc.identifier.doi | 10.1002/btpr.151 | en_US |
dc.identifier.source | Biotechnology Progress | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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