Show simple item record

Efficient tumour formation by single human melanoma cells

dc.contributor.authorQuintana, Elsaen_US
dc.contributor.authorShackleton, Marken_US
dc.contributor.authorSabel, Michael S.en_US
dc.contributor.authorFullen, Douglas R.en_US
dc.contributor.authorJohnson, Timothy M.en_US
dc.contributor.authorMorrison, Sean J.en_US
dc.date.accessioned2009-06-01T17:47:31Z
dc.date.available2009-06-01T17:47:31Z
dc.date.issued2008-12-04en_US
dc.identifier.citationQuintana, Elsa; Shackleton, Mark; Sabel, Michael S.; Fullen, Douglas R.; Johnson, Timothy M.; Morrison, Sean J.. (2008) "Efficient tumour formation by single human melanoma cells." Nature 456(7222): 593-U33. <http://hdl.handle.net/2027.42/62970>en_US
dc.identifier.issn0028-0836en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/62970
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19052619&dopt=citationen_US
dc.description.abstractA fundamental question in cancer biology is whether cells with tumorigenic potential are common or rare within human cancers. Studies on diverse cancers, including melanoma, have indicated that only rare human cancer cells ( 0.1 - 0.0001%) form tumours when transplanted into non- obese diabetic/ severe combined immunodeficiency ( NOD/ SCID) mice. However, the extent to which NOD/ SCID mice underestimate the frequency of tumorigenic human cancer cells has been uncertain. Here we show that modified xenotransplantation assay conditions, including the use of more highly immunocompromised NOD/ SCID interleukin- 2 receptor gamma chain null (Il2rg(-/-)) mice, can increase the detection of tumorigenic melanoma cells by several orders of magnitude. In limiting dilution assays, approximately 25% of unselected melanoma cells from 12 different patients, including cells from primary and metastatic melanomas obtained directly from patients, formed tumours under these more permissive conditions. In single- cell transplants, an average of 27% of unselected melanoma cells from four different patients formed tumours. Modifications to xenotransplantation assays can therefore dramatically increase the detectable frequency of tumorigenic cells, demonstrating that they are common in some human cancers.en_US
dc.description.sponsorshipHoward Hughes Medical Institute ; Allen H. Blondy Research Fellowship ; Lewis and Lillian Becker ; University of Michigan Comprehensive Cancer Center ; National Institutes of Health [CA46592]; University of Michigan Flow Cytometry Core Facility ; N. McAnsh and the University of Michigan Cancer Centre Histology Core ; National Institute of Diabetes, Digestive, and Kidney Diseases [NIH5P60- DK20572]; Michigan Diabetes Research and Training Center ; Spanish Ministry of Education ; Marie Curie Outgoing International Fellowship from the European Commission ; Australian National Health and Medical Research Council ; Human Frontiers Science Program and Australia Posten_US
dc.description.sponsorshipThis work was supported by the Howard Hughes Medical Institute and by the Allen H. Blondy Research Fellowship. The University of Michigan Melanoma Bank was supported by a gift from Lewis and Lillian Becker. Flow cytometry was partly supported by the University of Michigan Comprehensive Cancer Center grant from the National Institutes of Health CA46592. We thank: D. Adams, M. White and the University of Michigan Flow Cytometry Core Facility for support; N. McAnsh and the University of Michigan Cancer Centre Histology Core for histological studies; G. K. Smyth for assistance with statistics; and Z. Azizan for support with tissue collection. Antibody production was supported in part by the National Institute of Diabetes, Digestive, and Kidney Diseases, grant NIH5P60- DK20572 to the Michigan Diabetes Research and Training Center. Some antibodies were provided by Caltag or by eBioscience to screen for cancer stem- cell markers. Human primary melanocyte cultures were provided by M. Soengas. Human mesenchymal stem cells were provided by Z. Wang and P. Krebsbach. E. Q. was supported by the Spanish Ministry of Education and the Marie Curie Outgoing International Fellowship from the European Commission. M. S. was supported by the Australian National Health and Medical Research Council, the Human Frontiers Science Program and Australia Post.en_US
dc.format.extent622758 bytes
dc.format.extent2489 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherNature Publishing Groupen_US
dc.sourceNatureen_US
dc.titleEfficient tumour formation by single human melanoma cellsen_US
dc.typeArticleen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumMorrison, Sean J.] Univ Michigan, Howard Hughes Med Inst, Inst Life Sci, Dept Internal Med, Ann Arbor, MI 48109 USAen_US
dc.contributor.affiliationumMorrison, Sean J.] Univ Michigan, Ctr Cell Biol, Ann Arbor, MI 48109 USAen_US
dc.contributor.affiliationum[Sabel, Michael S.] Univ Michigan, Dept Surg, Ann Arbor, MI 48109 USAen_US
dc.contributor.affiliationum[Fullen, Douglas R.] Univ Michigan, Dept Pathol, Ann Arbor, MI 48109 USAen_US
dc.contributor.affiliationum[Johnson, Timothy M.] Univ Michigan, Dept Dermatol, Ann Arbor, MI 48109 USAen_US
dc.contributor.affiliationother[Quintana, Elsaen_US
dc.contributor.affiliationotherShackleton, Marken_US
dc.contributor.affiliationother[Quintana, Elsaen_US
dc.contributor.affiliationotherShackleton, Marken_US
dc.identifier.pmid19052619en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/62970/1/nature07567.pdf
dc.identifier.doihttp://dx.doi.org/10.1038/nature07567en_US
dc.identifier.sourceNatureen_US
dc.contributor.authoremailseanjm@umich.eduen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.