Functional Analysis of MycE and MycF, Two  O -Methyltransferases Involved in the Biosynthesis of Mycinamicin Macrolide Antibiotics

Functional Analysis of MycE and MycF, Two O -Methyltransferases Involved in the Biosynthesis of Mycinamicin Macrolide Antibiotics

Li, Shengying; Anzai, Yojiro; Kinoshita, Kenji; Kato, Fumio; Sherman, David H.

2009-05-25

Citation: Li, Shengying; Anzai, Yojiro; Kinoshita, Kenji; Kato, Fumio; Sherman, David H. (2009). "Functional Analysis of MycE and MycF, Two O -Methyltransferases Involved in the Biosynthesis of Mycinamicin Macrolide Antibiotics." ChemBioChem 10(8): 1297-1301. <http://hdl.handle.net/2027.42/62994>

Abstract: Mg motors : We characterized the in vitro function of MycE and MycF, two O -methyltransferases involved in the biosynthesis of mycinamicin antibiotics. Each enzyme was confirmed to be an S -adenosyl- L -methionine (SAM)-dependent deoxysugar methyltransferase. Their optimal activities require the presence of Mg 2+ . With the reconstituted in vitro assays, the order of mycinamicin VI→III→IV in the post-PKS (polyketide synthase) tailoring pathway of mycinamicin was unambiguously determined.

ISSN: 1439-4227, 1439-7633

DOIs: 10.1002/cbic.200900088

PMID: 19415708

URI: http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19415708&dopt=citation

Handle: http://hdl.handle.net/2027.42/62994

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