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Purification and Characterization of p68/70, Regeneration-Associated Proteins from Goldfish Brain

dc.contributor.authorLeski, Michael L.en_US
dc.contributor.authorAgranoff, Bernard W.en_US
dc.date.accessioned2010-04-01T14:45:33Z
dc.date.available2010-04-01T14:45:33Z
dc.date.issued1994-03en_US
dc.identifier.citationLeski, Michael L.; Agranoff, Bernard W. (1994). "Purification and Characterization of p68/70, Regeneration-Associated Proteins from Goldfish Brain." Journal of Neurochemistry 62(3): 1182-1191. <http://hdl.handle.net/2027.42/65206>en_US
dc.identifier.issn0022-3042en_US
dc.identifier.issn1471-4159en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/65206
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8113803&dopt=citationen_US
dc.description.abstractTwo acidic proteins (p68/70) previously shown to be associated with regeneration of the goldfish optic nerve were purified 887-fold from brain homogenates of Carassius auratus. Purification to homogeneity was achieved by sequential chromatography of a 100,000 g brain supernatant fraction on DEAE-Sephacel, Cu 2+ -charged iminodiacetic acid agarose, and gel filtration. The Stokes radius of the doublet was determined to be 5.8 nm, and the sedimentation coefficient calculated to be 5 2. From these values a molecular mass of 128 kDa and a frictional coefficient ratio of 1.6 were calculated. Chromatofocusing on a high-resolution DEAE column resolved the protein doublet into three dimeric species of p68, p68/70, and p70. These results indicate that the proteins are highly elongated and associate as homodimers or as a hetero-dimer. Subcellular localization and membrane extraction experiments indicated p68/70 to be a component of the plasma membrane associated primarily through hydro-phobic interactions. p68/70 demonstrated biphasic behavior in phase partition experiments using Triton 114. Analysis of hydrolytic products indicated p68/70 to be a glyco-protein, containing 11% carbohydrate.en_US
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dc.format.extent3110 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights1994 International Society for Neurochemistryen_US
dc.subject.otherGoldfishen_US
dc.subject.otherGlycoproteinen_US
dc.subject.otherPlasma Membraneen_US
dc.subject.otherRegenerationen_US
dc.subject.otherOptic Nerveen_US
dc.titlePurification and Characterization of p68/70, Regeneration-Associated Proteins from Goldfish Brainen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry and Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan, U.S.A.en_US
dc.identifier.pmid8113803en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/65206/1/j.1471-4159.1994.62031182.x.pdf
dc.identifier.doi10.1046/j.1471-4159.1994.62031182.xen_US
dc.identifier.sourceJournal of Neurochemistryen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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