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Characterization of Insulin-Like Growth Factor-I and Its Receptor and Binding Proteins in Transected Nerves and Cultured Schwann Cells

dc.contributor.authorCheng, Hsin-Linen_US
dc.contributor.authorRandolph, Ann E.en_US
dc.contributor.authorYee, Douglasen_US
dc.contributor.authorDelafontaine, Patricken_US
dc.contributor.authorTennekoon, Gihan I.en_US
dc.date.accessioned2010-04-01T14:56:02Z
dc.date.available2010-04-01T14:56:02Z
dc.date.issued1996-02en_US
dc.identifier.citationCheng, Hsin-Lin; Randolph, Ann; Yee, Douglas; Delafontaine, Patrick; Tennekoon, Gihan (1996). "Characterization of Insulin-Like Growth Factor-I and Its Receptor and Binding Proteins in Transected Nerves and Cultured Schwann Cells." Journal of Neurochemistry 66(2): 525-536. <http://hdl.handle.net/2027.42/65391>en_US
dc.identifier.issn0022-3042en_US
dc.identifier.issn1471-4159en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/65391
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8592122&dopt=citationen_US
dc.description.abstractThe insulin-like growth factors (IGFs) are trophic factors whose growth-promoting actions are mediated via the IGF-I receptor and modulated by six IGF binding proteins (IGFBPs). In this study, we observed increased transcripts of both IGF-I and IGF-I receptor after rat sciatic nerve transection. Schwann cells (SCs) were the main source of IGF-I and IGFBP-5 immunoreactivity until 7 days after nerve transection, when invading macrophages in the distal nerve stumps were strongly IGF-I positive. In vitro, IGF-I promoted SC mitogenesis. Northern analysis revealed that SCs expressed IGF-I receptor and IGFBP-5. IGF-I treatment increased the intensity of IGFBP-5 without affecting gene expression. Des(1–3)IGF-I, an IGF-I analogue with low affinity for IGFBP, had no such effect. Incubation of recombinant human IGFBP-5 with SC conditioned media revealed IGF-I protection of IGFBP-5 from proteolysis, implying the presence of an IGFBP-5 protease in SC conditioned media. Collectively, these data support the concept that, in response to nerve injury, invading macrophages produce IGF-I and SC express the IGF-I receptor, to facilitate regeneration. This regenerative process may be augmented further by the ability of SC to secrete IGFBPs, which in turn may increase local IGF-I bioavailability.en_US
dc.format.extent1477044 bytes
dc.format.extent3110 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherBlackwell Science Ltden_US
dc.rightsBlackwell Science Incen_US
dc.subject.otherInsulin-like Growth Factoren_US
dc.subject.otherReceptoren_US
dc.subject.otherBinding Proteinen_US
dc.subject.otherSchwann Cellsen_US
dc.subject.otherNerve Transectionen_US
dc.titleCharacterization of Insulin-Like Growth Factor-I and Its Receptor and Binding Proteins in Transected Nerves and Cultured Schwann Cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationother† Emory University, Atlanta, Georgia, U.S.A.en_US
dc.identifier.pmid8592122en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/65391/1/j.1471-4159.1996.66020525.x.pdf
dc.identifier.doi10.1046/j.1471-4159.1996.66020525.xen_US
dc.identifier.sourceJournal of Neurochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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