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Properties of a CDP-Diglyceride Hydrolase from Guinea Pig Brain

dc.contributor.authorRittenhouse, Harry G.en_US
dc.contributor.authorSeguin, Edward B.en_US
dc.contributor.authorFisher, Stephen K.en_US
dc.contributor.authorAgranoff, Bernard W.en_US
dc.date.accessioned2010-04-01T14:56:57Z
dc.date.available2010-04-01T14:56:57Z
dc.date.issued1981-03en_US
dc.identifier.citationRittenhouse, Harry G.; Seguin, Edward B.; Fisher, Stephen K.; Agranoff, Bernard W. (1981). "Properties of a CDP-Diglyceride Hydrolase from Guinea Pig Brain." Journal of Neurochemistry 36(3): 991-999. <http://hdl.handle.net/2027.42/65407>en_US
dc.identifier.issn0022-3042en_US
dc.identifier.issn1471-4159en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/65407
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6110708&dopt=citationen_US
dc.description.abstractEnzymatic hydrolysis of the pyrophosphate bond of CDP-diglyceride (CDP-DG), previously shown to occur in bacteria, is demonstrable in mammalian tissues. Activity was enriched in a lysosomal fraction obtained from guinea pig cerebral cortex and was purified 92-fold relative to the homogenate by a combination of XM-300 ultrafiltration and DEAE-cellulose column chromatography. When incubated with CDP-dipalmitin, the purified enzyme produced stoichiometric amounts of CMP and phosphatidate. dCDP-DG served as a substrate, while ADP-DG was an inhibitor, as were 5′-AMP and 5′-dAMP. CDP-DG hydrolysis was not affected by the presence of excess amounts of CDP-choline, CDP-glycerol, sodium pyrophosphate, or cyclic 3′,5′-AMP.en_US
dc.format.extent864206 bytes
dc.format.extent3110 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherBlackwell Publishing Ltden_US
dc.rights1981 International Society for Neurochemistry Ltd.en_US
dc.subject.otherCDP-diglyceride Hydrolaseen_US
dc.subject.otherLysosomesen_US
dc.subject.otherPhosphatidateen_US
dc.subject.otherCytidine 5′-Monophosphateen_US
dc.subject.otherAdenosine 5′-Monophosphateen_US
dc.titleProperties of a CDP-Diglyceride Hydrolase from Guinea Pig Brainen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumNeuroscience Laboratory, Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, 1103 E. Huron, Ann Arbor, Michigan 48109, U.S.A.en_US
dc.identifier.pmid6110708en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/65407/1/j.1471-4159.1981.tb01691.x.pdf
dc.identifier.doi10.1111/j.1471-4159.1981.tb01691.xen_US
dc.identifier.sourceJournal of Neurochemistryen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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