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Enhanced Coupling of Neonatal Muscarinic Receptors in Rat Brain to Phosphoinositide Turnover

dc.contributor.authorHeacock, Anne M.en_US
dc.contributor.authorFisher, Stephen K.en_US
dc.contributor.authorAgranoff, Bernard W.en_US
dc.date.accessioned2010-04-01T15:34:36Z
dc.date.available2010-04-01T15:34:36Z
dc.date.issued1987-06en_US
dc.identifier.citationHeacock, Anne M.; Fisher, Stephen K.; Agranoff, Bernard W. (1987). "Enhanced Coupling of Neonatal Muscarinic Receptors in Rat Brain to Phosphoinositide Turnover." Journal of Neurochemistry 48(6): 1904-1911. <http://hdl.handle.net/2027.42/66062>en_US
dc.identifier.issn0022-3042en_US
dc.identifier.issn1471-4159en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/66062
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3033155&dopt=citationen_US
dc.description.abstractThe relationship between the density of the muscarinic receptor in developing rat cerebral cortex and its coupling to phosphoinositide turnover is examined. Tissue slices from rats of various ages were incubated with myo- [2- 3 H]inositol, and the effect of carbamoylcholine on the release of total inositol phosphates was determined. Binding of [ 3 H]quinuclidinyl benzilate was determined in the same tissue. Although muscarinic receptor density in day-18 embryonic cortex was only 5% of that in the adult, the maximal response of stimulated phosphoinositide turnover to carbamoylcholine (1–10 m M ) was at the adult level (i.e., threefold increase). Comparison of the dependence of the turnover on carbamoylcholine concentration revealed that in neonates, the dose-response curve was shifted to the left, giving a half-maximal effect at concentrations approximately tenfold lower than that in the adult. In addition, the partial muscarinic agonists oxotremorine-2 and bethanechol were both more efficacious in young rats than in adults. The differences could not be accounted for either by alterations in agonist affinity for the receptor or by the presence of “spare” muscarinic receptors. These results indicate that muscarinic receptors in fetal and newborn rat cerebral cortex are more efficiently coupled to stimulation of phosphoinositide turnover than in the adult.en_US
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dc.publisherBlackwell Publishing Ltden_US
dc.rights1987 International Society for Neurochemistryen_US
dc.subject.otherInositol Phospholipiden_US
dc.subject.otherMuscarinic Receptorsen_US
dc.subject.otherRat Cerebral Cortex Developmenten_US
dc.titleEnhanced Coupling of Neonatal Muscarinic Receptors in Rat Brain to Phosphoinositide Turnoveren_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumNeuroscience Laboratory, University of Michigan, Ann Arbor, Michigan, U.S.A.en_US
dc.identifier.pmid3033155en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/66062/1/j.1471-4159.1987.tb05754.x.pdf
dc.identifier.doi10.1111/j.1471-4159.1987.tb05754.xen_US
dc.identifier.sourceJournal of Neurochemistryen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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