Modulation of Opioid Receptor Binding by Cis and Trans Fatty Acids
dc.contributor.author | Remmers, Ann E. | en_US |
dc.contributor.author | Nordby, Gordon L. | en_US |
dc.contributor.author | Medzihradsky, Fedor | en_US |
dc.date.accessioned | 2010-04-01T15:35:55Z | |
dc.date.available | 2010-04-01T15:35:55Z | |
dc.date.issued | 1990-12 | en_US |
dc.identifier.citation | Remmers, Ann E.; Nordby, Gordon L.; Medzihradsky, Fedor (1990). "Modulation of Opioid Receptor Binding by Cis and Trans Fatty Acids." Journal of Neurochemistry 55(6): 1993-2000. <http://hdl.handle.net/2027.42/66085> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/66085 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2172466&dopt=citation | en_US |
dc.description.abstract | In synaptosomal brain membranes, the addition of oleic acid (cis), elaidic acid (trans), and the cis and trans isomers of vaccenic acid, at a concentration of 0.87 Μmol of lipid/mg of protein, strongly reduced the B max and, to a lesser degree, the binding affinity of the Μ-selective opioid [ 3 H]Tyr-D-Ala-Gly-(Me)Phe-Gly-ol ([ 3 H]DAMGO). At comparable membrane content, the cis isomers of the fatty acids were more potent than their trans counterparts in inhibiting ligand binding and in decreasing membrane microviscosity, both at the membrane surface and in the core. However, trans- vaccenic acid affected opioid receptor binding in spite of just marginally altering membrane microviscosity. If the receptors were uncoupled from guanine nucleotide regulatory protein, an altered inhibition profile was obtained: the impairment of K D by the fatty acids was enhanced and that of B max reduced. Receptor interaction of the Δ-opioid [ 3 H](D-Pen 2 ,D-Pen 5 )enkephalin was modulated by lipids to a greater extent than that of [ 3 H]DAMGO: saturable binding was abolished by both oleic and elaidic acids. The binding of [ 3 H]naltrexone was less susceptible to inhibition by the fatty acids, particularly in the presence of sodium. In the absence of this cation, however, cis -vaccenic acid abolished the low-affinity binding component of [ 3 H]naltrexone. These findings support the membrane model of opioid receptor sequestration depicting different ionic environments for the Μ- and Δ-binding sites. The results of this work show distinct modulation of different types and molecular states of opioid receptor by fatty acids through mechanisms involving membrane fluidity and specific interactions with membrane constituents. | en_US |
dc.format.extent | 848649 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1990 International Society for Neurochemistry | en_US |
dc.subject.other | Μ- and Δ-opioid receptors | en_US |
dc.subject.other | Ligand-receptor interaction | en_US |
dc.subject.other | Cis and trans fatty acids | en_US |
dc.subject.other | Membrane lipid content | en_US |
dc.subject.other | Membrane microviscosity | en_US |
dc.title | Modulation of Opioid Receptor Binding by Cis and Trans Fatty Acids | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Biological Chemistry and Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 2172466 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/66085/1/j.1471-4159.1990.tb05787.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.1990.tb05787.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.identifier.citedreference | Abood L. G., Butler M., and Reynolds D. ( 1980 ) Effect of calcium and physical state of neural membranes on phosphatidylserine requirement for opioid binding. Mol. Pharmacol. 17, 290 – 294. | en_US |
dc.identifier.citedreference | Gordon, L. M., eds ( 1988 ) Physiological Regulation of Membrane Fluidity. Alan R. Liss, Inc., New York. | en_US |
dc.identifier.citedreference | Ben-Arie N., Gileadi C., and Schramm M. ( 1988 ) Interaction of the Β-adrenergic receptor with G s following delipidation. Specific lipid requirements for G s activation and GTPase function. Eur. J. Pharmacol. 176, 649 – 654. | en_US |
dc.identifier.citedreference | Bradford M. M. ( 1976 ) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248 – 254. | en_US |
dc.identifier.citedreference | Cahill A. L. and Medzihradsky F. ( 1976 ) Interaction of central nervous system drugs with synaptosomal transport processes. Biochem. Pharmacol. 25, 2257 – 2264. | en_US |
dc.identifier.citedreference | Carruthers A. and Melchior D. L. ( 1986 ) How bilayer lipids affect membrane protein activity. Trends Biochem. Sci. 11, 331 – 335. | en_US |
dc.identifier.citedreference | Carruthers A. and Melchior D. L. ( 1988 ) Role of the bilayer lipids in governing membrane transport processes, in Lipid Domains and the Relationship to Membrane Function ( Gordon L. M., eds ), pp. 201 – 225. Alan R. Liss, Inc., New York. | en_US |
dc.identifier.citedreference | Clark M. J., Carter B. D., and Medzihradsky F. ( 1988 ) Selectivity of ligand binding to opioid receptors in brain membranes from the rat, monkey and guinea pig. Eur. J. Pharmacol. 148, 343 – 351. | en_US |
dc.identifier.citedreference | Fischel S. V. and Medzihradsky F. ( 1981 ) Scatchard analysis of opiate receptor binding. Mol. Pharmacol. 20, 269 – 279. | en_US |
dc.identifier.citedreference | Gillan M. G. C., Kosterlitz H. W., and Paterson S. J. ( 1980 ) Comparison of the binding characteristics of tritiated opiates and opioid peptides. Br. J. Pharmacol. 70, 481 – 490. | en_US |
dc.identifier.citedreference | Hasegawa J.-I., Loh H. H., and Lee N. M. ( 1987 ) Lipid requirement for Μ opioid receptor binding. J. Neurochem. 49, 1007 – 1012. | en_US |
dc.identifier.citedreference | Hauser H., Guyer W., and Howell K. ( 1979 ) Lateral distribution of negatively charged lipids in lecithin membranes. Clustering of fatty acids. Biochemistry 18, 3285 – 3291. | en_US |
dc.identifier.citedreference | Ho W. K. K. and Cox B. M. ( 1982 ) Reduction of opioid binding in neuroblastoma × glioma cells grown in medium containing unsaturated fatty acids. Biochim. Biophys. Acta 688, 211 – 217. | en_US |
dc.identifier.citedreference | Karnovsky M. J., Kleinfield A. M., Hoover R. L., and Klausner R. D. ( 1982 ) The concept of lipid domains in membranes. J. Cell Biol. 94, 1 – 6. | en_US |
dc.identifier.citedreference | Kates M and Manson L. A. ( 1984 ) Membrane fluidity. Plenum Press, New York. | en_US |
dc.identifier.citedreference | Kuhry J.-G., Fonteneau P., Duportail G., Maechling C., and Laustriat G. ( 1983 ) TMA-DPH: a suitable fluorescence polarization probe for specific plasma membrane fluidity studies in intact living cells. Cell Biophys. 5, 129 – 140. | en_US |
dc.identifier.citedreference | Law P. Y., Griffon M. T., Koehler J. E., and Loh H. H. ( 1983 ) Attenuation of enkephalin activity in neuroblastoma × glioma NG108–15 hybrid cells by phospholipases. J. Neurochem. 40, 267 – 275. | en_US |
dc.identifier.citedreference | Lazar D. F. and Medzihradsky F. ( 1990 ) Differential inhibition of delta opiate binding and low K m GTPase stimulation by phos-pholipase A 2 treatment. Prog. Clin. Biol. Res. 328, 113 – 116. | en_US |
dc.identifier.citedreference | Lin H.-K. and Simon E. J. ( 1978 ) Phospholipase A inhibition of opiate receptor binding can be reversed by albumin. Nature 271, 383 – 384. | en_US |
dc.identifier.citedreference | Lowry O. H., Rosebrough N. J., Farr A. L., and Randall R. J. ( 1951 ) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265 – 275. | en_US |
dc.identifier.citedreference | McGee, R. and Kenimer J. G. ( 1982 ) The effects of exposure to unsaturated fatty acids on opiate receptors, prostaglandin E 1 receptors, and adenylate cyclase activity of neuroblastoma × glioma hybrid cells. Mol. Pharmacol. 22, 360 – 368. | en_US |
dc.identifier.citedreference | McOsker C. C., Weiland G. A., and Zilversmit D. B. ( 1983 ) Inhibition of hormone-stimulated adenylate cyclase activity after altering turkey erythrocyte phospholipid composition with a nonspecific lipid transfer protein. J. Biol. Chem. 258, 13017 – 13026. | en_US |
dc.identifier.citedreference | McPhail L. C., Clayton C. C., and Snyderman R. ( 1984 ) A potential second messenger role for unsaturated fatty acids: activation of Ca 2+ -dependent protein kinase. Science 224, 622 – 625. | en_US |
dc.identifier.citedreference | Medzihradsky F. ( 1989 ) Modulation of opioid receptor mechanisms by membrane lipids: an investigative approach. Adv. Biosci. 75, 41 – 44. | en_US |
dc.identifier.citedreference | Morrison W. R. and Smith L. M. ( 1964 ) Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. J. Lipid Res. 5, 600 – 608. | en_US |
dc.identifier.citedreference | Ordway R. W., Walsh J. V., and Singer J. J. ( 1989 ) Arachidonic acid and other fatty acids directly activate potassium channels in smooth muscle cells. Science 244, 1176 – 1179. | en_US |
dc.identifier.citedreference | Orly J. and Schramm M. ( 1975 ) Fatty acids as modulators of membrane function: catecholamine activated adenylate cyclase of the turkey erythrocyte. Proc. Natl. Acad. Sci. USA 72, 3433 – 3437. | en_US |
dc.identifier.citedreference | Ortiz A. and Gomez-Fernandez J. C. ( 1987 ) A differential scanning calorimetry study of the interaction of free fatty acids with phospholipid membranes. Chem. Phys. Lipids 45, 75 – 91. | en_US |
dc.identifier.citedreference | Radin N. ( 1969 ) Preparation of lipid extracts, in Methods in Enzymology. Vol. XIV ( Lowenstein J., ed. ), pp. 245 – 254. Academic Press, New York. | en_US |
dc.identifier.citedreference | Remmers A. E. and Medzihradsky F. ( 1987 ) Modulation of ligand binding to opioid receptors by membrane lipids. Abstracts, International Narcotics Research Conference, Adelaide, Australia. | en_US |
dc.identifier.citedreference | Rimon G., Hanski E., Braun S., and Levitzki A. ( 1978 ) Mode of coupling between hormone receptors and adenylate cyclase elucidated by modulation of membrane fluidity. Nature 276, 394 – 396. | en_US |
dc.identifier.citedreference | Sargent D. F. and Schwyzer R. ( 1986 ) Membrane lipid phase as catalyst for peptide-receptor interactions. Proc. Natl. Acad. Sci. USA 83, 5774 – 5778. | en_US |
dc.identifier.citedreference | Sargent D. F., Bean J. W., and Schwyzer R. ( 1988 ) Conformation and orientation of regulatory peptides on lipid membranes. Key to the molecular mechanism of receptor selection. Biophys. Chem. 31, 183 – 193. | en_US |
dc.identifier.citedreference | Schiller P. W., Nguyen T. M.-D., Chung N. N., and Lemieux C. ( 1989 ) Dermorphin analogues carrying an increased positive net charge in their “message” domain display extremely high Μ opioid receptor selectivity. J. Med. Chem. 32, 698 – 703. | en_US |
dc.identifier.citedreference | Schwyzer R. ( 1986 ) Molecular mechanism of opioid receptor selection. Biochemistry 25, 6335 – 6342. | en_US |
dc.identifier.citedreference | Seifert R., Schachtele C., Rosenthal W., and Schultz G. ( 1988 ) Activation of protein kinase C by cis- and trans-fatty acids and its potentiation by diacylglycerol. Biochem. Biophys. Res. Commun. 154, 20 – 26. | en_US |
dc.identifier.citedreference | Shinitzky M. ( 1984 ) Physiology of Membrane Fluidity, Vol. I, CRC Press, Inc., Boca Raton, Honda. | en_US |
dc.identifier.citedreference | Shinitzky M. and Barenholz Y. ( 1978 ) Fluidity parameters of lipid regions determined by fluorescence polarization. Biochim. Biophys. Acta 515, 367 – 394. | en_US |
dc.identifier.citedreference | Stubbs C. D. and Smith A. D. ( 1984 ) The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function. Biochim. Biophys. Acta 779, 89 – 137. | en_US |
dc.identifier.citedreference | Werling L. L., Puttfarcken P. S., and Cox B. M. ( 1988 ) Multiple agonist-affinity states of opioid receptors: regulation of binding by guanyl nucleotides in guinea pig cortical, NG108–15, and 7314c cell membranes. Mol. Pharmacol. 33, 423 – 451. | en_US |
dc.identifier.citedreference | Wilkinson L. ( 1988 ) SYSTAT: The System for Statistics. SYSTAT Inc., Evanston, Illinois. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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