Cytoskeletal and Phosphoinositide Requirements for Muscarinic Receptor Signaling to Focal Adhesion Kinase and Paxillin
dc.contributor.author | Linseman, Daniel A. | en_US |
dc.contributor.author | McEwen, Edward L. | en_US |
dc.contributor.author | Sorensen, Scott D. | en_US |
dc.date.accessioned | 2010-04-01T15:41:37Z | |
dc.date.available | 2010-04-01T15:41:37Z | |
dc.date.issued | 1998-03 | en_US |
dc.identifier.citation | Linseman, Daniel A.; McEwen, Edward L.; Sorensen, Scott D. (1998). "Cytoskeletal and Phosphoinositide Requirements for Muscarinic Receptor Signaling to Focal Adhesion Kinase and Paxillin." Journal of Neurochemistry 70(3): 940-950. <http://hdl.handle.net/2027.42/66183> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/66183 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9489713&dopt=citation | en_US |
dc.description.abstract | The mechanism whereby agonist occupancy of muscarinic cholinergic receptors elicits an increased tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin has been examined. Addition of oxotremorine-M to SH-SY5Y neuroblastoma cells resulted in rapid increases in the phosphorylation of FAK ( t 1/2 = 2 min) and paxillin that were independent of integrin-extracellular matrix interactions, cell attachment, and the production of phosphoinositide-derived second messengers. In contrast, the increased tyrosine phosphorylations of FAK and paxillin were inhibited by inclusion of either cytochalasin D or mevastatin, agents that disrupt the cytoskeleton. Furthermore, phosphorylation of FAK and paxillin could be prevented by addition of either wortmannin or LY-294002, under conditions in which the synthesis of phosphatidylinositol 4-phosphate was markedly attenuated. These results indicate that muscarinic receptor-mediated increases in the tyrosine phosphorylation of FAK and paxillin in SH-SY5Y neuroblastoma cells depend on both the maintenance of an actin cytoskeleton and the ability of these cells to synthesize phosphoinositides. | en_US |
dc.format.extent | 1353221 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Science Ltd | en_US |
dc.rights | Blackwell Science Inc | en_US |
dc.subject.other | Actin Polymerization | en_US |
dc.subject.other | Phosphatidylinositol 4-phosphate | en_US |
dc.subject.other | Cytochalasin | en_US |
dc.subject.other | LY-294002 | en_US |
dc.subject.other | Mevastatin | en_US |
dc.subject.other | Wortmannin | en_US |
dc.title | Cytoskeletal and Phosphoinositide Requirements for Muscarinic Receptor Signaling to Focal Adhesion Kinase and Paxillin | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | * Neuroscience Laboratory, Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 9489713 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/66183/1/j.1471-4159.1998.70030940.x.pdf | |
dc.identifier.doi | 10.1046/j.1471-4159.1998.70030940.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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