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Glutamate Decarboxylases in Nonneural Cells of Rat Testis and Oviduct: Differential Expression of GAD 65 and GAD 67

dc.contributor.authorTillakaratne, Niranjala J. K.en_US
dc.contributor.authorErlander, Mark G.en_US
dc.contributor.authorCollard, Michael W.en_US
dc.contributor.authorGreif, Karen F.en_US
dc.contributor.authorTobin, Allan J.en_US
dc.date.accessioned2010-04-01T15:43:15Z
dc.date.available2010-04-01T15:43:15Z
dc.date.issued1992-02en_US
dc.identifier.citationTillakaratne, Niranjala J. K.; Erlander, Mark G.; Collard, Michael W.; Greif, Karen F.; Tobin, Allan J. (1992). "Glutamate Decarboxylases in Nonneural Cells of Rat Testis and Oviduct: Differential Expression of GAD 65 and GAD 67 ." Journal of Neurochemistry 58(2): 618-627. <http://hdl.handle.net/2027.42/66211>en_US
dc.identifier.issn0022-3042en_US
dc.identifier.issn1471-4159en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/66211
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1729406&dopt=citationen_US
dc.description.abstractΓ-Aminobutyric acid (GABA) and its synthetic enzyme, glutamate decarboxylase (GAD), are not limited to the nervous system but are also found in nonneural tissues. The mammalian brain contains at least two forms of GAD (GAD 67 and GAD 65 ), which differ from each other in size, sequence, immunoreactivity, and their interaction with the cofactor pyridoxal 5′-phosphate (PLP). We used cDNAs and antibodies specific to GAD 65 and GAD 67 to study the molecular identity of GADs in peripheral tissues. We detected GAD and GAD mRNAs in rat oviduct and testis. In oviduct, the size of GAD, its response to PLP, its immunoreactivity, and its hybridization to specific RNA and DNA probes all indicate the specific expression of the GAD 65 gene. In contrast, rat testis expresses the GAD 67 gene. The GAD in these two reproductive tissues is not in neurons but in nonneural cells. The localization of brain GAD and GAD mRNAs in the mucosal epithelial cells of the oviduct and in spermatocytes and spermatids of the testis shows that GAD is not limited to neurons and that GABA may have functions other than neurotransmission.en_US
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dc.publisherBlackwell Publishing Ltden_US
dc.rights1992 International Society for Neurochemistryen_US
dc.subject.otherGlutamate Decarboxylaseen_US
dc.subject.otherΓ-Aminobutyric Aciden_US
dc.subject.otherOviducten_US
dc.subject.otherTestisen_US
dc.subject.otherMRNAsen_US
dc.titleGlutamate Decarboxylases in Nonneural Cells of Rat Testis and Oviduct: Differential Expression of GAD 65 and GAD 67en_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationum† Mental Health Research Institute, University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationother* Department of Biology, University of California, Los Angeles, Los Angeles, Californiaen_US
dc.contributor.affiliationother† Neuroscience Program, University of California, Los Angeles, Los Angeles, Californiaen_US
dc.contributor.affiliationotherMolecular Biology Institute, University of California, Los Angeles, Los Angeles, Californiaen_US
dc.contributor.affiliationother¶ Brain Research Institute, University of California, Los Angeles, Los Angeles, Californiaen_US
dc.contributor.affiliationother§ Department of Biology, Bryn Mawr College, Bryn Mawr, Pennsylvania, U.S.A.en_US
dc.identifier.pmid1729406en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/66211/1/j.1471-4159.1992.tb09763.x.pdf
dc.identifier.doi10.1111/j.1471-4159.1992.tb09763.xen_US
dc.identifier.sourceJournal of Neurochemistryen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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