View Item 

Show simple item record

Barium and Calcium Stimulate Secretion from Digitonin-Permeabilized Bovine Adrenal Chromaffin Cells by Similar Pathways

dc.contributor.authorTerBush, Daniel R.en_US
dc.contributor.authorHolz, Ronald W.en_US
dc.date.accessioned2010-04-01T15:49:44Z
dc.date.available2010-04-01T15:49:44Z
dc.date.issued1992-02en_US
dc.identifier.citationTerBush, Daniel R.; Holz, Ronald W. (1992). "Barium and Calcium Stimulate Secretion from Digitonin-Permeabilized Bovine Adrenal Chromaffin Cells by Similar Pathways." Journal of Neurochemistry 58(2): 680-687. <http://hdl.handle.net/2027.42/66323>en_US
dc.identifier.issn0022-3042en_US
dc.identifier.issn1471-4159en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/66323
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1729410&dopt=citationen_US
dc.description.abstractWe compared the characteristics of secretion stimulated by EGTA-buffered Ba 2+ - and Ca 2+ -containing solutions in digitonin-permeabilized bovine adrenal chromaffin cells. Half-maximal secretion occurred at approximately 100 Μ M Ba 2+ or 1 Μ M Ca 2+ . Ba 2+ -stimulated release was not due to release of sequestered intracellular Ca 2+ because at a constant free Ba 2+ concentration, increasing unbound EGTA did not diminish the extent of release due to Ba 2+ . The maximal extents of Ba 2+ - and Ca 2+ -dependent secretion in the absence of MgATP were identical. MgATP enhanced Ba 2+ -induced secretion to a lesser extent than Ca 2+ -induced secretion. Half-maximal concentrations of Ba 2+ and Ca 2+ , when added together to cells, yielded approximately additive amounts of secretion. Maximal concentrations of Ba 2+ and Ca 2+ when added together to cells for 2 or 15 min were not additive. Tetanus toxin inhibited Ba 2+ - and Ca 2+ -dependent secretion to a similar extent. Ba 2+ , unlike Ca 2+ , did not activate polyphosphoinositide-specific phospholipase C. These data indicate that (1) Ba 2+ directly stimulates exocytosis, (2) Ba 2+ -induced secretion is stimulated to a lesser extent than Ca 2+ -dependent secretion by MgATP, (3) Ba 2+ and Ca 2+ use similar pathways to trigger exocytosis, and (4) exocytosis from permeabilized cells does not require activation of polyphosphoinositide-specific phospholipase C.en_US
dc.format.extent833320 bytes
dc.format.extent3110 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherBlackwell Publishing Ltden_US
dc.rights1992 International Society for Neurochemistryen_US
dc.subject.otherATPen_US
dc.subject.otherBariumen_US
dc.subject.otherCalciumen_US
dc.subject.otherChromaffin Cellen_US
dc.subject.otherExocytosisen_US
dc.subject.otherTetanus Toxinen_US
dc.titleBarium and Calcium Stimulate Secretion from Digitonin-Permeabilized Bovine Adrenal Chromaffin Cells by Similar Pathwaysen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pharmacology, University of Michigan, Ann Arbor, Michigan, U.S.A.en_US
dc.identifier.pmid1729410en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/66323/1/j.1471-4159.1992.tb09771.x.pdf
dc.identifier.doi10.1111/j.1471-4159.1992.tb09771.xen_US
dc.identifier.sourceJournal of Neurochemistryen_US
dc.identifier.citedreferenceAli S. M., Geisow M. J., and Burgoyne R. D. ( 1989 ) A role for calpactin in calcium-dependent exocytosis in adrenal chromaffin cells. Nature 340, 313 – 315.en_US
dc.identifier.citedreferenceAmy C. M. and Kirshner N. ( 1981 ) Phosphorylation of adrenal medulla cell proteins in conjunction with stimulation of catecholamine secretion. J. Neurochem. 36, 847 – 854.en_US
dc.identifier.citedreferenceArtalejo C. R., Garcia A. G., and Aunis D. ( 1987 ) Chromaffin cell calcium channel kinetics measured isotopically through fast calcium, strontium and barium fluxes. J. Biol. Chem. 262, 915 – 926.en_US
dc.identifier.citedreferenceBjerrum J., Schwarzenbach G., and SillÉn L. G. ( 1957 ) Stability Constants, Part I: Organic Ligands, p. 98. The Chemical Society, London.en_US
dc.identifier.citedreferenceBrooks J. C. and Treml S. ( 1983 ) Catecholamine secretion by chemically skinned cultured chromaffin cells. J. Neurochem. 40, 468 – 473.en_US
dc.identifier.citedreferenceChang D., Hsieh P. S., and Dawson D. C. ( 1988 ) Calcium: a program in BASIC for calculating the composition of solutions with specified free concentrations of calcium, magnesium and other divalent cations. Comput. Biol. Med. 18, 351 – 366.en_US
dc.identifier.citedreferenceChao S.-H., Suzuki Y., Zysk J. R., and Cheung W. Y. ( 1984 ) Activation of calmodulin by various metal cations as a function of ionic radius. Mol. Pharmacol. 26, 75 – 82.en_US
dc.identifier.citedreferenceCote A., Doucet J. P., and TrifarÓ J. M. ( 1986 ) Phosphorylation and dephosphorylation of chromaffin cell proteins in response to stimulation. Neuroscience 19, 629 – 645.en_US
dc.identifier.citedreferenceCreutz C. E., Pazoles C. J., and Pollard H. B. ( 1979 ) Self-association of synexin in the presence of calcium: correlation with synexin-induced membrane fusion and examination of the structure of synexin aggregates. J. Biol. Chem. 254, 553 – 558.en_US
dc.identifier.citedreferenceDavidson J. S., Wakefield I., King J. A., and Millar R. P. ( 1987 ) Barium-induced LH release from chicken pituitary cells: synergism with phorbol ester. J. Endocrinol. 114, 11 – 16.en_US
dc.identifier.citedreferenceDouglas W. W. and Rubin R. P. ( 1964a ) The effects of alkaline earths and other divalent cations on adrenal medullary secretion. J. Physiol. 175, 231 – 241.en_US
dc.identifier.citedreferenceDouglas W. W. and Rubin R. P. ( 1964b ) Stimulant action of barium on the adrenal medulla. Nature 203, 305 – 307.en_US
dc.identifier.citedreferenceDunn L. A. and Holz R. W. ( 1983 ) Catecholamine secretion from digitonin-treated adrenal chromaffin cells. J. Biol. Chem. 258, 4989 – 4993.en_US
dc.identifier.citedreferenceEberhard D. A. and Holz R. W. ( 1987 ) Cholinergic stimulation of inositol phosphate formation in bovine adrenal chromaffin cells: distinct nicotinic and muscarinic mechanisms. J. Neurochem. 49, 1634 – 1643.en_US
dc.identifier.citedreferenceEberhard D. A., Cooper C. L., Low M. G., and Holz R. W. ( 1990 ) Evidence that the inositol phospholipids are necessary for exocytosis. Biochem. J. 268, 15 – 25.en_US
dc.identifier.citedreferenceFrye R. A. and Holz R. W. ( 1984 ) The relationship between arachidonic acid release and catecholamine secretion from cultured bovine adrenal chromaffin cells. J. Neurochem. 43, 146 – 150.en_US
dc.identifier.citedreferenceFrye R. A. and Holz R. W. ( 1985 ) Arachidonic acid release and catecholamine secretion from digitonin-treated chromaffin cells: effects of micromolar calcium, phorbol ester, and protein alkylating agents. J. Neurochem. 44, 265 – 273.en_US
dc.identifier.citedreferenceHales C. N. and Milner R. D. G. ( 1968 ) Cations and the secretion of insulin from rabbit pancreas in vitro. J. Physiol. (Lond.) 199, 177 – 197.en_US
dc.identifier.citedreferenceHeldman E., Levine M., Raveh L., and Pollard H. B. ( 1989 ) Barium ions enter chromaffin cells via voltage-dependent calcium channels and induce secretion by a mechanism independent of calcium. J. Biol. Chem. 264, 7914 – 7920.en_US
dc.identifier.citedreferenceHolz R. W., Senter R. A., and Frye R. A. ( 1982 ) Relationship between Ca 2+ uptake and catecholamine secretion in primary dissociated cultures of adrenal medulla. J. Neurochem. 39, 635 – 646.en_US
dc.identifier.citedreferenceHolz R. W., Bittner M. A., Peppers S. C., Senter R. A., and Eberhard D. A. ( 1989 ) MgATP-independent and MgATP-dependent exocytosis. J. Biol. Chem. 264, 5412 – 5419.en_US
dc.identifier.citedreferenceIzumi F., Toyohira Y., Yanagihara N., Wada A., and Kobayashi H. ( 1986 ) Barium-evoked release of catecholamines from digitonin-permeabilized adrenal medullary cells. Neurosci. Lett. 69, 172 – 175.en_US
dc.identifier.citedreferenceKao L.-S. and Schneider A. S. ( 1986 ) Calcium mobilization and catecholamine secretion in adrenal chromaffin cells. J. Biol. Chem. 261, 4881 – 4888.en_US
dc.identifier.citedreferenceKilpatrick D. L., Slepetis R. J., Corcoran J. J., and Kirshner N. ( 1982 ) Calcium uptake and catecholamine secretion by cultured bovine adrenal medulla cells. J. Neurochem. 38, 427 – 435.en_US
dc.identifier.citedreferenceKnight D. E. and Baker P. F. ( 1982 ) Calcium-dependence of catecholamine release from bovine adrenal medullary cells after exposure to intense electric fields. J. Membr. Biol. 68, 107 – 140.en_US
dc.identifier.citedreferenceKnight D. E. and Kesteven N. T. ( 1983 ) Evoked transient intracellular free Ca 2+ changes and secretion in isolated adrenal medullary cells. Proc. R. Soc. Lond. [Biol. Sci.] 218, 177 – 199.en_US
dc.identifier.citedreferenceKnight D. E., Tonge D. A., and Baker P. F. ( 1985 ) Inhibition of exocytosis in bovine adrenal medullary cells by botulinum toxin type D. Nature 317, 719 – 721.en_US
dc.identifier.citedreferenceKnight D. E., Sugden D., and Baker P. F. ( 1988 ) Evidence implicating protein kinase C in exocytosis from electropermeabilized bovine chromaffin cells. J. Membr. Biol. 104, 21 – 34.en_US
dc.identifier.citedreferenceLee S. A. and Holz R. W. ( 1986 ) Protein phosphorylation and secretion in digitonin-permeabilized adrenal chromaffin cells: effects of micromolar Ca 2+, phorbol esters, and diacylglycerol. J. Biol. Chem. 261, 17089 – 17098.en_US
dc.identifier.citedreferenceMatthies H. J., Palfrey H. C., and Miller R. J. ( 1988 ) Calmodulin- and protein phosphorylation-independent release of catecholamines from PC12 cells. FEBS Lett. 229, 238 – 242.en_US
dc.identifier.citedreferenceMorgan A. and Burgoyne R. D. ( 1990 ) Stimulation of Ca 2+ -independent catecholamine secretion from digitonin-permeabilized bovine adrenal chromaffin cells by guanine nucleotide analogues. Relationship to arachidonate release. Biochem. J. 269, 521 – 526.en_US
dc.identifier.citedreferenceMorita K., Ishii S., Uda H., and Oka M. ( 1988 ) Requirement of ATP for exocytotic release of catecholamines from digitonin-permeabilized adrenal chromaffin cells. J. Neurochem. 50, 644 – 648.en_US
dc.identifier.citedreferencePocotte S. L., Holz R. W., and Ueda T. ( 1986 ) Cholinergic receptor-mediated phosphorylation and activation of tyrosine hydroxylase in cultured bovine adrenal chromaffin cells. J. Neurochem. 46, 610 – 622.en_US
dc.identifier.citedreferenceSarafian T., Aunis D., and Bader M.-F. ( 1987 ) Loss of proteins from digitonin-permeabilized adrenal chromaffin cells essential for exocytosis. J. Biol. Chem. 262, 16671 – 16676.en_US
dc.identifier.citedreferenceSasakawa N., Nakaki T., Yamamoto S., and Kato R. ( 1989 ) Calcium uptake-dependent and -independent mechanisms of inositol trisphosphate formation in adrenal chromaffin cells: comparative studies with high K +, carbamylcholine and angiotensin II. Cell. Signal. 1, 75 – 84.en_US
dc.identifier.citedreferenceSÜdhof T. C., Walker J. H., and Obrocki J. ( 1982 ) Calelectrin self-aggregates and promotes membrane aggregation in the presence of calcium. EMBO J. 1, 1167 – 1170.en_US
dc.identifier.citedreferenceTerBush D. R. and Holz R. W. ( 1990 ) Activation of protein kinase C is not required for exocytosis from bovine adrenal chromaffin cells: the effects of PKC(19-31), Ca/CaM kinase II(291-317) and staurosporine. J. Biol. Chem. 265, 21179 – 21184.en_US
dc.identifier.citedreferencevan der Merwe P. A., Millar R. P., and Davidson J. S. ( 1990 ) Calcium stimulates luteinizing-hormone (lutropin) exocytosis by a mechanism independent of protein kinase C. Biochem. J. 268, 493 – 498.en_US
dc.identifier.citedreferenceWaymire J. C., Bennett W. F., Boehme R., Hanteins L., Gilmer-Waymire K., and Haycock J. ( 1983 ) Bovine adrenal chromaffin cells: high-yield purification and viability in suspension culture. J. Neurosci. Methods 7, 329 – 351.en_US
dc.identifier.citedreferenceWilson S. P. and Kirshner N. ( 1977 ) The acetylcholine receptor of the adrenal medulla. J. Neurochem. 28, 687 – 695.en_US
dc.identifier.citedreferenceWilson S. P. and Kirshner N. ( 1983 ) Calcium-evoked secretion from digitonin-permeabilized adrenal medullary chromafin cells. J. Biol. Chem. 258, 4994 – 5000.en_US
dc.identifier.citedreferenceZaks W. J. and Creutz C. E. ( 1990 ) Annexin-chromaffin granule membrane interations: a comparative study of synexin, p32 and p67. Biochem. Biophys. Acta 1029, 149 – 160.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
j.1471-4159.1992.tb09771.x.pdf
Size:
813.7KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.