Effects of Tetanus Toxin on Catecholamine Release from Intact and Digitonin-Permeabilized Chromaffin Cells
dc.contributor.author | Bittner, Mary A. | en_US |
dc.contributor.author | Holz, Ronald W. | en_US |
dc.date.accessioned | 2010-04-01T15:57:16Z | |
dc.date.available | 2010-04-01T15:57:16Z | |
dc.date.issued | 1988-08 | en_US |
dc.identifier.citation | Bittner, Mary A.; Holz, Ronald W. (1988). "Effects of Tetanus Toxin on Catecholamine Release from Intact and Digitonin-Permeabilized Chromaffin Cells." Journal of Neurochemistry 51(2): 451-456. <http://hdl.handle.net/2027.42/66452> | en_US |
dc.identifier.issn | 0022-3042 | en_US |
dc.identifier.issn | 1471-4159 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/66452 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3392539&dopt=citation | en_US |
dc.description.abstract | Tetanus exotoxin inhibited Ca 2+ -dependent cate-cholamine secretion in a dose-dependent manner in digito-nin-permeabilized chromaffin cells. The inhibition was specific for tetanus exotoxin and the B fragment of tetanus toxin; the C fragment had no effect. Inhibition required the introduction of toxin into the cell, and was not seen when intact cells were preincubated with the toxin or toxin fragments. The degree of inhibition was related to the length of preincubation with toxin, as well as the concentration of toxin used. A short preincubation with toxin was sufficient to inhibit secretion, and the continued presence of toxin in the incubation medium was not required during the incubation with Ca 2+ . The inhibition of secretion by tetanus toxin or the B fragment was not overcome with increasing Ca 2+ concentrations. Tetanus toxin also inhibited catechol-amine secretion enhanced by phorbol ester-induced activation of protein kinase C. Thus, the toxin or a proteolytic fragment of the toxin can enter digitonin-permeabilized cells to interact with a component of the Ca 2+ -dependent exocytotic pathway to inhibit secretion. | en_US |
dc.format.extent | 628319 bytes | |
dc.format.extent | 3110 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1988 International Society for Neurochemistry Ltd. | en_US |
dc.subject.other | Tetanus Toxin | en_US |
dc.subject.other | Chromaffin Cell | en_US |
dc.subject.other | Catecholamine | en_US |
dc.subject.other | Exocytosis | en_US |
dc.subject.other | Digitonin-permeabilized | en_US |
dc.subject.other | Botulinum Toxin | en_US |
dc.title | Effects of Tetanus Toxin on Catecholamine Release from Intact and Digitonin-Permeabilized Chromaffin Cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 3392539 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/66452/1/j.1471-4159.1988.tb01059.x.pdf | |
dc.identifier.doi | 10.1111/j.1471-4159.1988.tb01059.x | en_US |
dc.identifier.source | Journal of Neurochemistry | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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