Separation of Fucosyltransferase and Endogenous Acceptors of Fucose from Human Parotid Saliva

Abstract

Using gel filtration on Sephadex G-100 and column chromatography on CM-cellulose, fractions containing either endogenous acceptor of L-fucose or fucosyltransferase were obtained from human parotid saliva. Hydrolysis with mild acid followed by Smith degradation increased the fucosyltransferase activity of the fractions that contained endogeneous acceptor.