Expression of HOXDIO Gene in Normal Endometrium and Endometrial Adenocarcinoma
dc.contributor.author | Osborne, Janet | en_US |
dc.contributor.author | Hu, Changzi | en_US |
dc.contributor.author | Hawley, Colleen | en_US |
dc.contributor.author | Underwood, Lowell | en_US |
dc.contributor.author | O'Brien, Timothy | en_US |
dc.contributor.author | Baker, Vicki | en_US |
dc.date.accessioned | 2010-04-14T14:19:35Z | |
dc.date.available | 2010-04-14T14:19:35Z | |
dc.date.issued | 1998 | en_US |
dc.identifier.citation | Osborne, Janet; Hu, Changzi; Hawley, Colleen; Underwood, Lowell; O'Brien, Timothy; Baker, Vicki (1998). "Expression of HOXDIO Gene in Normal Endometrium and Endometrial Adenocarcinoma." Journal of the Society for Gynecologic Investigation 5(5): 277-280. <http://hdl.handle.net/2027.42/69122> | en_US |
dc.identifier.issn | 1071-5576 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/69122 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9773404&dopt=citation | en_US |
dc.description.abstract | Objective: Hox genes encode DNA transcription regulatory that contain a conserved 61 amino acid protein called the homeodomain. Although best known for their role in cellular differentiation during embryonic development, aberrant expression of these genes has been associated with hematologic and solid neoplasms. The purpose of this study was to determine the relative expression of HOXD10 in huamn endometrial adenocarcionmas. Methods: mRNA was isolated from 7 normal endometrial specimens and 28 endometrial adenocarcinoma specimens. cDNA was synthesized using randon hexamer primers. The expression of HOXD10 relative to βtubulin (internal control) was assessed by densitometric comparison of co-amplified Phosphorus-32 (32P) labeled gene products separated by agarose gel electrophoesis. Direct sequencing of purified HOXD10 polymerase chain reaction product was also performed. Results: The sequence of the purified HOXD10 product corresponds to the known DNA sequence reported in the National Institutes of Health Gene Bank. mRNA expression of HOXD10 relative to β-tubulin is significantly lower in endometrial carcinomas than in normal endometrium. Furthermore, the ratio of HOXD10 to β-tubulin expression varies inversely with the histologic grade of the tumor (P = .0009). Conclusion: Cancer is a multistep proces involving aberrant expression of genes that regulate cell growth and differentiation. Human HOXD10 gene expression is altered in endometrial carcinoma and varies with the histologic grade of differentiation. This observation supports the theory that homeobox genes play a role in oncogenesis. | en_US |
dc.format.extent | 3108 bytes | |
dc.format.extent | 785626 bytes | |
dc.format.mimetype | text/plain | |
dc.format.mimetype | application/pdf | |
dc.publisher | Sage Publications | en_US |
dc.subject.other | Homeobox Genes | en_US |
dc.subject.other | HOXD10 | en_US |
dc.subject.other | Oncogenesis | en_US |
dc.subject.other | Endometrial Cancer | en_US |
dc.title | Expression of HOXDIO Gene in Normal Endometrium and Endometrial Adenocarcinoma | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Gynecologic Oncology, University of Michiga N. Ann Arbor, Michigan and Departments of Obsteries and Gynecology, and Biocgemistry and Moclecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas; Lehigh Valley Women's Cancer Center, 1011 Pond Road, Allentown, PA 18104 | en_US |
dc.contributor.affiliationum | Division of Gynecologic Oncology, University of Michiga N. Ann Arbor, Michigan and Departments of Obsteries and Gynecology, and Biocgemistry and Moclecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas | en_US |
dc.identifier.pmid | 9773404 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/69122/2/10.1177_107155769800500509.pdf | |
dc.identifier.doi | 10.1177/107155769800500509 | en_US |
dc.identifier.source | Journal of the Society for Gynecologic Investigation | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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