Low-cost monochromatic microsecond flash microbeam apparatus for single-cell photolysis of rhodopsin or other photolabile pigments
dc.contributor.author | Sullivan, Jack M. | en_US |
dc.date.accessioned | 2010-05-06T23:08:32Z | |
dc.date.available | 2010-05-06T23:08:32Z | |
dc.date.issued | 1998-02 | en_US |
dc.identifier.citation | Sullivan, Jack M. (1998). "Low-cost monochromatic microsecond flash microbeam apparatus for single-cell photolysis of rhodopsin or other photolabile pigments." Review of Scientific Instruments 69(2): 527-539. <http://hdl.handle.net/2027.42/71060> | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/71060 | |
dc.description.abstract | Delivery of intense, brief flashes of monochromatic light are required in single-cell physiological experiments to photolyze cellular chromophores or pigments. In the xenon flash instrument constructed, flashes are collimated, made monochromatic with selectable bandpass filters and imaged into a small-core fiber. The flash is transmitted over meters to the epiflourescent port of a microscope where additional optics again collimate the beam. The objective lens of the microscope functions to condense flash energy into a microbeam in the specimen (field) plane and to image the cell under parafocal conditions. Spot diameters are 228 and 166 μm (full width half maximum) for 40×40× and 60×60× objectives. Flash intensities can be measured with this instrument during experiments using the microscope phase/differential interference contrast condenser to couple the microbeam to a calibrated photodiode. Flash intensities between 108108 and 109 photons/μm2109photons/μm2 were achieved across the near-ultraviolet/visible spectrum. Flash durations were under 20 μs with a short-arc 7 J flash tube. Shielding and fiber transfer permit delivery of intense flashes without electromagnetic noise to the electrophysiological recording apparatus. Flashes generated with this instrument activated intramolecular charge motions (early receptor currents) in the visual pigment, rhodopsin, expressed from transgenes in single cultured cells. © 1998 American Institute of Physics. | en_US |
dc.format.extent | 3102 bytes | |
dc.format.extent | 200315 bytes | |
dc.format.mimetype | text/plain | |
dc.format.mimetype | application/pdf | |
dc.publisher | The American Institute of Physics | en_US |
dc.rights | © The American Institute of Physics | en_US |
dc.title | Low-cost monochromatic microsecond flash microbeam apparatus for single-cell photolysis of rhodopsin or other photolabile pigments | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Physics | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Ophthalmology, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Departments of Ophthalmology and Biochemistry/Molecular Biology, SUNY Health Science Center, Syracuse, New York 13210 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/71060/2/RSINAK-69-2-527-1.pdf | |
dc.identifier.doi | 10.1063/1.1148460 | en_US |
dc.identifier.source | Review of Scientific Instruments | en_US |
dc.identifier.citedreference | S. Zamenhof, Rev. Sci. Instrum. RSINAK14, 17 (1943). | en_US |
dc.identifier.citedreference | R. B. Uretz and R. P. Perry, Rev. Sci. Instrum. RSINAK28, 861 (1957). | en_US |
dc.identifier.citedreference | M. W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J. B. Rattner, M. Kitzes, M. Hammer-Wilson, L.-H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P. J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, and G. S. Berns, Science SCIEAS213, 505 (1981). | en_US |
dc.identifier.citedreference | R. J. Leslie and J. D. Pickett-Heaps, J. Cell Biol. JCLBA396, 548 (1983). | en_US |
dc.identifier.citedreference | P. Wilson and A. Forer, Biochem. Cell Biol. BCBIEQ65, 363 (1987). | en_US |
dc.identifier.citedreference | O. G. Stonington, T. P. Spurck, J. A. Snyder, and J. D. Pickett-Heaps, Protoplasma PROTA5153, 62 (1989). | en_US |
dc.identifier.citedreference | W. Stuhmer and W. Almers, Proc. Natl. Acad. Sci. USA PNASA679, 946 (1982). | en_US |
dc.identifier.citedreference | S. Hestrin and J. I. Korenbrot, J. Neurosci. JNRSDS10, 1967 (1990). | en_US |
dc.identifier.citedreference | E. Niggli and W. J. Lederer, Biophys. J. BIOJAU59, 1123 (1991). | en_US |
dc.identifier.citedreference | M. S. Kirby, R. W. Hadley, and W. J. Lederer, Pflugers Arch. Ges. Physiol. Menschen Tiere AGPPAS427, 169 (1994). | en_US |
dc.identifier.citedreference | D. Bownds, Nature (London) NATUAS216, 1178 (1967). | en_US |
dc.identifier.citedreference | O. P. Hamill, A. Marty, E. Neher, B. Sakmann, and F. J. Sigworth, Pflugers Arch. Ges. Physiol. Menschen Tiere AGPPAS391, 85 (1981). | en_US |
dc.identifier.citedreference | H. J. A. Dartnall, Br. Med. Bull. BMBUAQ9, 24 (1953). | en_US |
dc.identifier.citedreference | A. Kropf, Vision Res. VISRAM7, 811 (1967). | en_US |
dc.identifier.citedreference | R. W. Schoenlein, L. A. Peteanu, R. A. Mathies, and C. V. Shank, Science SCIEAS254, 412 (1991). | en_US |
dc.identifier.citedreference | R. R. Birge, C. M. Einterz, H. M. Knapp, and L. P. Murray, Biophys. J. BIOJAU53, 367 (1988). | en_US |
dc.identifier.citedreference | J. H. Parkes and P. A. Liebman, Biochemistry BICHAW23, 5054 (1984). | en_US |
dc.identifier.citedreference | C. Longstaff, R. Calhoon, and R. A. Rando, Proc. Natl. Acad. Sci. USA PNASA683, 4209 (1986). | en_US |
dc.identifier.citedreference | A. A. Lamola, T. Yamane, and A. Zipp, Biochemistry BICHAW13, 738 (1974). | en_US |
dc.identifier.citedreference | D. S. Cafiso and W. L. Hubbell, Biophys. J. BIOJAU30, 243 (1980). | en_US |
dc.identifier.citedreference | J. Nathans, C. J. Weitz, N. Agarwal, I. Nir, and D. S. Papermaster, Vision Res. VISRAM29, 907 (1989). | en_US |
dc.identifier.citedreference | C.-H. Sung, B. G. Schneider, N. Agarwal, D. S. Papermaster, and J. Nathans, Proc. Natl. Acad. Sci. USA PNASA688, 8840 (1991). | en_US |
dc.identifier.citedreference | C.-H. Sung, C. M. Davenport, and J. Nathans, J. Biol. Chem. JBCHA3268, 26645 (1993). | en_US |
dc.identifier.citedreference | K. Fahmy, F. Jager, M. Beck, T. A. Zvyaga, and T. P. Sakmar, Proc. Natl. Acad. Sci. USA PNASA690, 10206 (1993). | en_US |
dc.identifier.citedreference | P. Rath, L. L. J. DeCaluwe, P. H. M. Bovee-Geurts, W. J. DeGrip, and K. J. Rothschild, Biochemistry BICHAW32, 10277 (1993). | en_US |
dc.identifier.citedreference | J. W. Lewis, F. J. G. M. van Kuijk, T. E. Thorgeirsson, and D. S. Kliger, Biochemistry BICHAW30, 11372 (1991). | en_US |
dc.identifier.citedreference | O. Kuwata, C. Yuan, and T. G. Ebrey, Invest. Ophthalmol. Visual Sci. IOVSDA38, 593S (1997). | en_US |
dc.identifier.citedreference | G. B. Cohen, D. D. Oprian, and P. R. Robinson, Biochemistry BICHAW31, 12592 (1992). | en_US |
dc.identifier.citedreference | C. J. Weitz and J. Nathans, Biochemistry BICHAW32, 14176 (1993). | en_US |
dc.identifier.citedreference | S. Arnis, K. Fahmy, K. P. Hofmann, and T. P. Sakmar, J. Biol. Chem. JBCHA3269, 23879 (1994). | en_US |
dc.identifier.citedreference | H. W. Trissl, Biophys. Struct. Mech. 8, 213 (1982a). | en_US |
dc.identifier.citedreference | J. D. Spalink and H. Stieve, Biophys. Struct. Mech. 6, 171 (1980). | en_US |
dc.identifier.citedreference | P. Hochstrate, M. Lindau, and H. Ruppel, Biophys. J. BIOJAU38, 53 (1982). | en_US |
dc.identifier.citedreference | H. W. Trissl, Mtds. Enzymol. 81, 431 (1982b). | en_US |
dc.identifier.citedreference | J. E. Lisman and H. Bering, J. Gen. Physiol. JGPLAD70, 621 (1977). | en_US |
dc.identifier.citedreference | T. G. Ebrey, Vision Res. VISRAM8, 965 (1968). | en_US |
dc.identifier.citedreference | A. L. Hodgkin and P. M. O’Bryan, J. Physiol. (London) JPHYA7267, 737 (1977). | en_US |
dc.identifier.citedreference | C. L. Makino, W. R. Taylor, and D. A. Baylor, J. Physiol. (London) JPHYA7442, 761 (1991). | en_US |
dc.identifier.citedreference | R. A. Cone, Science SCIEAS155, 1128 (1967). | en_US |
dc.identifier.citedreference | R. A. Cone, Exp. Eye Res. EXERA68, 246 (1969). | en_US |
dc.identifier.citedreference | R. A. Cone and W. L. Pak, in Handbook of Sensory Physiology: Principles of Receptor Physiology, edited by W. R. Lowenstein (Springer, Berlin, 1971), Vol. 1, Chap. 12, pp. 345–365. | en_US |
dc.identifier.citedreference | F. Bezanilla and E. Stefani, Annu. Rev. Biophys. Biomol. Struct. 23, 819 (1994). | en_US |
dc.identifier.citedreference | H. W. Trissl, Photochem. Photobiol. PHCBAP29, 579 (1979). | en_US |
dc.identifier.citedreference | L. A. Drachev, G. R. Kalamkarov, A. D. Kaulen, M. A. Ostrovsky, and V. P. Skulachev, Eur. J. Biochem. EJBCAI117, 471 (1981). | en_US |
dc.identifier.citedreference | T. P. Williams, J. Gen. Physiol. JGPLAD47, 679 (1964). | en_US |
dc.identifier.citedreference | T. P. Williams, Vision Res. VISRAM5, 633 (1965). | en_US |
dc.identifier.citedreference | E. N. Pugh, Jr., J. Physiol. (London) JPHYA7248, 393 (1975a). | en_US |
dc.identifier.citedreference | E. N. Pugh, Jr., J. Physiol. (London) JPHYA7248, 413 (1975b). | en_US |
dc.identifier.citedreference | W. Ernst and C. M. Kemp, Vision Res. VISRAM19, 363 (1979). | en_US |
dc.identifier.citedreference | S. Inoue, Video Microscopy (Plenum, New York, 1986), pp. 93–148. | en_US |
dc.identifier.citedreference | A. C. Hardy and F. M. Young, J. Opt. Soc. Am. JOSAAH39, 265 (1949). | en_US |
dc.identifier.citedreference | J. H. Goncz, J. Appl. Phys. JAPIAU36, 742 (1965). | en_US |
dc.identifier.citedreference | J. H. Goncz and P. B. Newell, J. Opt. Soc. Am. JOSAAH56, 87 (1962). | en_US |
dc.identifier.citedreference | M. A. Gusinow, J. Appl. Phys. JAPIAU44, 4567 (1973). | en_US |
dc.identifier.citedreference | M. A. Gusinow, J. Appl. Phys. JAPIAU46, 4847 (1975). | en_US |
dc.identifier.citedreference | H. E. Edgerton, R. Bonazoli, and J. T. Lamb, J. SMPTE JSMTA463, 15 (1954). | en_US |
dc.identifier.citedreference | H. E. Edgerton, J. H. Goncz, and P. W. Jameson, in Proceedings of the Sixth International Congress on High-Speed Photography, edited by J. G. A. deGraaf and P. Tegelaar (H. D. Tjeenk Willink & Zoon N. V., Haarlem, 1963), pp. 143–151. | en_US |
dc.identifier.citedreference | A. Buck, R. Erickson, and F. Barnes, J. Appl. Phys. JAPIAU34, 2115 (1963). | en_US |
dc.identifier.citedreference | J. P. Markiewicz and J. L. Emmett, J. Quantum Electron. QE-2, 707 (1966). | en_US |
dc.identifier.citedreference | D. E. Perlman, Rev. Sci. Instrum. RSINAK37, 340 (1966). | en_US |
dc.identifier.citedreference | T. Efthymiopoulos and B. K. Garside, Appl. Opt. APOPAI16, 70 (1977). | en_US |
dc.identifier.citedreference | G. W. Ellis, J. Cell Biol. JCLBA383, 303a (1979). | en_US |
dc.identifier.citedreference | G. W. Ellis, J. Cell Biol. JCLBA3101, 83a (1985). | en_US |
dc.identifier.citedreference | H. J. A. Dartnall, Vision Res. VISRAM8, 339 (1968). | en_US |
dc.identifier.citedreference | G. W. LeCompte and H. E. Edgerton, J. Appl. Phys. JAPIAU27, 1427 (1956). | en_US |
dc.identifier.citedreference | R. A. Knudson, Am. Lab. June, 50 (1990). | en_US |
dc.identifier.citedreference | G. Rapp and K. Guth, Pflugers Arch. Ges. Physiol. Menschen Tiere AGPPAS411, 200 (1988). | en_US |
dc.identifier.citedreference | J. M. Nerbonne, in Optical Methods in Cell Physiology, edited by P. DeWeer and B. M. Salzberg (Wiley, New York, 1986), Vol. 40, Society of General Physiologists Series, Chap. 24, pp. 417–445. | en_US |
dc.identifier.citedreference | H. G. Khorana, B. E. Knox, E. Nasi, R. Swanson, and D. A. Thompson, Proc. Natl. Acad. Sci. USA PNASA685, 7917 (1988). | en_US |
dc.identifier.citedreference | H. W. Trissl, A. Darszon, and M. Montal, Proc. Natl. Acad. Sci. USA PNASA674, 207 (1977). | en_US |
dc.identifier.citedreference | M. Lindau, P. Hochstrate, and H. Ruppel, FEBS Lett. FEBLAL112, 17 (1980). | en_US |
dc.identifier.citedreference | J. S. Huebner, R. T. Arrieta, I. C. Arrieta, and P. M. Pachori, Photochem. Photobiol. PHCBAP39, 191 (1984). | en_US |
dc.identifier.citedreference | H. A. Lester, L. D. Chabala, A. M. Gurney, and R. E. Sheridan, in Optical Methods in Cell Physiology, edited by P. DeWeer and B. M. Salzberg (Wiley, New York, 1986), Vol. 40, Society of General Physiologists Series, Chap. 25, pp. 448–462. | en_US |
dc.identifier.citedreference | J. H. Kaplan and A. P. Somlyo, TINS 12, 54 (1989). | en_US |
dc.identifier.citedreference | J. A. McCray and D. R. Trentham, Annu. Rev. Biophys. Biophys. Chem. ARBCEY18, 239 (1989). | en_US |
dc.owningcollname | Physics, Department of |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.