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Repression of motility during fimbrial expression: identification of 14 mrpJ gene paralogues in Proteus mirabilis

dc.contributor.authorPearson, Melanie M.en_US
dc.contributor.authorMobley, Harry L. T.en_US
dc.date.accessioned2010-06-01T18:19:36Z
dc.date.available2010-06-01T18:19:36Z
dc.date.issued2008-07en_US
dc.identifier.citationPearson, Melanie M.; Mobley, Harry L. T. (2008). "Repression of motility during fimbrial expression: identification of 14 mrpJ gene paralogues in Proteus mirabilis ." Molecular Microbiology 69(2): 548-558. <http://hdl.handle.net/2027.42/71536>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/71536
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18630347&dopt=citationen_US
dc.description.abstractProteus mirabilis alternates between motile and adherent forms. MrpJ, a transcriptional regulator previously reported to repress motility, is encoded at the 3′ end of the mrp fimbrial operon in P. mirabilis . Sequencing of the P. mirabilis genome revealed 14 additional paralogues of mrpJ , 10 of which are associated with fimbrial operons. Twelve of these genes, when overexpressed, repressed motility; several distinct patterns of swarming motility were noted. Expression of 10 of the 14 mrpJ paralogues repressed flagellin (FlaA) synthesis. Alignment of the predicted amino acid sequences of MrpJ and its 14 paralogues revealed a conserved consensus motif (SQQQFSRYE) within the helix-turn-helix domain. Site-directed mutagenesis of these residues coupled with linker insertion mutagenesis of MrpJ confirmed the importance of this domain for repression of motility. Gel shift assays demonstrated that MrpJ and another paralogue UcaJ bind directly to the promoter region of the flagellar master regulator flhDC . Thus, P. mirabilis appears to use a related mechanism to inhibit motility during the production of at least 10 of its predicted fimbriae.en_US
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dc.publisherBlackwell Publishing Ltden_US
dc.rightsJournal compilation © 2008 Blackwell Publishingen_US
dc.titleRepression of motility during fimbrial expression: identification of 14 mrpJ gene paralogues in Proteus mirabilisen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.identifier.pmid18630347en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71536/1/j.1365-2958.2008.06307.x.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71536/2/MMI_6307_sm_Figure_S1_and_Tables_S1-S3.pdf
dc.identifier.doi10.1111/j.1365-2958.2008.06307.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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