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HtpG, the Porphyromonas gingivalis HSP-90 homologue, induces the chemokine CXCL8 in human monocytic and microvascular vein endothelial cells

dc.contributor.authorShelburne, Charles E.en_US
dc.contributor.authorCoopamah, Malini D.en_US
dc.contributor.authorSweier, Domenica G.en_US
dc.contributor.authorAn, Florence Y. -P.en_US
dc.contributor.authorLopatin, Dennis E.en_US
dc.date.accessioned2010-06-01T18:35:24Z
dc.date.available2010-06-01T18:35:24Z
dc.date.issued2007-06en_US
dc.identifier.citationShelburne, Charles E.; Coopamah, Malini D.; Sweier, Domenica G.; An, Florence Y.-P.; Lopatin, Dennis E. (2007). "HtpG, the Porphyromonas gingivalis HSP-90 homologue, induces the chemokine CXCL8 in human monocytic and microvascular vein endothelial cells." Cellular Microbiology 9(6): 1611-1619. <http://hdl.handle.net/2027.42/71792>en_US
dc.identifier.issn1462-5814en_US
dc.identifier.issn1462-5822en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/71792
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17346315&dopt=citationen_US
dc.description.abstractCXCL8 (interlukin 8, IL-8) has a diverse spectrum of biological activities including T cell, neutrophil and basophil chemotactic properties. It is produced by a wide variety of cell types and plays a significant role in the initiation of the acute inflammatory response. During inflammation, CXCL8 attracts and activates leukocytes at the site of infection leading to leukocyte infiltration, which can lead to tissue damage. Porphyromonas gingivalis , an aetiological agent of periodontitis, induces production of CXCL8 from several types of cells via its LPS and outer membrane proteins. Bacterial chaperones elicit a strong pro-inflammatory response in cells of the innate immune system. In P. gingivalis the htpG gene codes for the homologue of human Hsp90, a chaperone that associates with transcription factors, hormone receptors and protein kinases, affecting signal transduction pathways. CXCL8 mRNA and CXCL8 protein production was induced in monocytic/human microvascular vein endothelial cells treated with P. gingivalis cells or rHtpG protein. Blocking of receptors CD91 and TLR4 reduced the production of CXCL8 by rHtpG either using receptor-specific antibody or by siRNA silencing. Pre-incubation of P. gingivalis rHtpG preparations with human anti-HtpG significantly inhibited CXCL8 production. A P. gingivalis HtpG disruption mutant also induced less CXCL8 mRNA and protein. These results suggest that P. gingivalis HtpG might be involved in CXCL8-mediated immunopathogenesis.en_US
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dc.format.extent3109 bytes
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dc.publisherBlackwell Publishing Ltden_US
dc.rights© 2007 The Authors; Journal compilation © 2007 Blackwell Publishing Ltden_US
dc.titleHtpG, the Porphyromonas gingivalis HSP-90 homologue, induces the chemokine CXCL8 in human monocytic and microvascular vein endothelial cellsen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumCariology, Restorative Sciences and Endodontics, School of Dentistry, The University of Michigan, Ann Arbor, MI 48108, USA.en_US
dc.contributor.affiliationotherDepartments of Biologic and Materials Sciences anden_US
dc.identifier.pmid17346315en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71792/1/j.1462-5822.2007.00897.x.pdf
dc.identifier.doi10.1111/j.1462-5822.2007.00897.xen_US
dc.identifier.sourceCellular Microbiologyen_US
dc.identifier.citedreferenceArgueta, J.G., Shiota, S., Yamaguchi, N., Masuhiro, Y., and Hanazawa, S. ( 2006 ) Induction of Porphyromonas gingivalis GroEL signaling via binding to Toll-like receptors 2 and 4. Oral Microbiol Immunol 21: 245 – 251.en_US
dc.identifier.citedreferenceBaggiolini, M. ( 1998 ) Chemokines and leukocyte traffic. Nature 392: 565 – 568.en_US
dc.identifier.citedreferenceBainbridge, B.W., Coats, S.R., and Darveau, R.P. ( 2002 ) Porphyromonas gingivalis lipopolysaccharide displays functionally diverse interactions with the innate host defense system. Ann Periodontol 7: 29 – 37.en_US
dc.identifier.citedreferenceBalasubramanian, A., Munshi, N., Koziel, M.J., Hu, Z., Liang, T.J., Groopman, J.E., and Ganju, R.K. ( 2005 ) Structural proteins of Hepatitis C virus induce interleukin 8 production and apoptosis in human endothelial cells. J Gen Virol 86: 3291 – 3301.en_US
dc.identifier.citedreferenceBarker, K.S., Liu, T., and Rogers, P.D. ( 2005 ) Coculture of THP-1 human mononuclear cells with Candida albicans results in pronounced changes in host gene expression. J Infect Dis 192: 901 – 912.en_US
dc.identifier.citedreferenceBasu, S., Binder, R.J., Ramalingam, T., and Srivastava, P.K. ( 2001 ) CD91 is a common receptor for heat shock proteins gp96, hsp90, hsp70, and calreticulin. Immunity 14: 303 – 313.en_US
dc.identifier.citedreferencevan Berkel, T.J., Out, R., Hoekstra, M., Kuiper, J., Biessen, E., and van Eck, M. ( 2005 ) Scavenger receptors: friend or foe in atherosclerosis? Curr Opin Lipidol 16: 525 – 535.en_US
dc.identifier.citedreferenceBulut, Y., Faure, E., Thomas, L., Karahashi, H., Michelsen, K.S., Equils, O., et al. ( 2002 ) Chlamydial heat shock protein 60 activates macrophages and endothelial cells through Toll-like receptor 4 and MD2 in a MyD88-dependent pathway. J Immunol 168: 1435 – 1440.en_US
dc.identifier.citedreferenceCairo, F., Gaeta, C., Dorigo, W., Oggioni, M.R., Pratesi, C., Pini Prato, G.P., and Pozzi, G. ( 2004 ) Periodontal pathogens in atheromatous plaques. A controlled clinical and laboratory trial. J Periodontal Res 39: 442 – 446.en_US
dc.identifier.citedreferenceCoats, S.R., Reife, R.A., Bainbridge, B.W., Pham, T.T., and Darveau, R.P. ( 2003 ) Porphyromonas gingivalis lipopolysaccharide antagonizes Escherichia coli lipopolysaccharide at toll-like receptor 4 in human endothelial cells. Infect Immun 71: 6799 – 6807.en_US
dc.identifier.citedreferenceCoulter, W.A., Coffey, A., Saunders, I.D., and Emmerson, A.M. ( 1990 ) Bacteremia in children following dental extraction. J Dent Res 69: 1691 – 1695.en_US
dc.identifier.citedreferenceCutler, C.W., Kalmar, J.R., and Arnold, R.R. ( 1991 ) Phagocytosis of virulent Porphyromonas gingivalis by human polymorphonuclear leukocytes requires specific immunoglobulin G. Infect Immun 59: 2097 – 2104.en_US
dc.identifier.citedreferenceCutler, C.W., Jotwani, R., Palucka, K.A., Davoust, J., Bell, D., and Banchereau, J. ( 1999 ) Evidence and a novel hypothesis for the role of dendritic cells and Porphyromonas gingivalis in adult periodontitis. J Periodontal Res 34: 406 – 412.en_US
dc.identifier.citedreferenceDa Costa, C.U., Wantia, N., Kirschning, C.J., Busch, D.H., Rodriguez, N., Wagner, H., and Miethke, T. ( 2004 ) Heat shock protein 60 from Chlamydia pneumoniae elicits an unusual set of inflammatory responses via Toll-like receptor 2 and 4 in vivo. Eur J Immunol 34: 2874 – 2884.en_US
dc.identifier.citedreferenceDaig, R., Andus, T., Aschenbrenner, E., Falk, W., Scholmerich, J., and Gross, V. ( 1996 ) Increased interleukin 8 expression in the colon mucosa of patients with inflammatory bowel disease. Gut 38: 216 – 222.en_US
dc.identifier.citedreferenceDarveau, R.P., and Hancock, R.E. ( 1983 ) Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains. J Bacteriol 155: 831 – 838.en_US
dc.identifier.citedreferenceDarveau, R.P., Arbabi, S., Garcia, I., Bainbridge, B., and Maier, R.V. ( 2002 ) Porphyromonas gingivalis lipopolysaccharide is both agonist and antagonist for p38 mitogen-activated protein kinase activation. Infect Immun 70: 1867 – 1873.en_US
dc.identifier.citedreferenceGangur, V., Birmingham, N.P., and Thanesvorakul, S. ( 2002 ) Chemokines in health and disease. Vet Immunol Immunopathol 86: 127 – 136.en_US
dc.identifier.citedreferenceGear, A.R., and Camerini, D. ( 2003 ) Platelet chemokines and chemokine receptors: linking hemostasis, inflammation, and host defense. Microcirculation 10: 335 – 350.en_US
dc.identifier.citedreferenceHajishengallis, G., Sharma, A., Russell, M.W., and Genco, R.J. ( 2002 ) Interactions of oral pathogens with toll-like receptors: possible role in atherosclerosis. Ann Periodontol 7: 72 – 78.en_US
dc.identifier.citedreferenceHaraszthy, V.I., Zambon, J.J., Trevisan, M., Zeid, M., and Genco, R.J. ( 2000 ) Identification of periodontal pathogens in atheromatous plaques. J Periodontol 71: 1554 – 1560.en_US
dc.identifier.citedreferenceHinode, D., Yoshioka, M., Tanabe, S., Miki, O., Masuda, K., and Nakamura, R. ( 1998 ) The GroEL-like protein from Campylobacter rectus: immunological characterization and interleukin-6 and -8 induction in human gingival fibroblast. FEMS Microbiol Lett 167: 1 – 6.en_US
dc.identifier.citedreferenceHuang, S., Mills, L., Mian, B., Tellez, C., McCarty, M., Yang, X.D., et al. ( 2002 ) Fully humanized neutralizing antibodies to interleukin-8 (ABX-IL8) inhibit angiogenesis, tumor growth, and metastasis of human melanoma. Am J Pathol 161: 125 – 134.en_US
dc.identifier.citedreferenceKaufmann, S.H. ( 1990 ) Heat shock proteins and the immune response. Immunol Today 11: 129 – 136.en_US
dc.identifier.citedreferenceKinane, D.F., Lappin, D.F., Koulouri, O., and Buckley, A. ( 1999 ) Humoral immune responses in periodontal disease may have mucosal and systemic immune features. Clin Exp Immunol 115: 534 – 541.en_US
dc.identifier.citedreferenceKoulouri, O., Lappin, D.F., Radvar, M., and Kinane, D.F. ( 1999 ) Cell division, synthetic capacity and apoptosis in periodontal lesions analysed by in situ hybridisation and immunohistochemistry. J Clin Periodontol 26: 552 – 559.en_US
dc.identifier.citedreferenceKozarov, E., Sweier, D., Shelburne, C., Progulske-Fox, A., and Lopatin, D. ( 2006 ) Detection of bacterial DNA in atheromatous plaques by quantitative PCR. Microbes Infect 8: 687 – 693.en_US
dc.identifier.citedreferenceLappin, D.F., McGregor, A.M., and Kinane, D.F. ( 2003 ) The systemic immune response is more prominent than the mucosal immune response in the pathogenesis of periodontal disease. J Clin Periodontol 30: 778 – 786.en_US
dc.identifier.citedreferenceLin, S.N., Ayada, K., Zhao, Y., Yokota, K., Takenaka, R., Okada, H., et al. ( 2005 ) Helicobacter pylori heat-shock protein 60 induces production of the pro-inflammatory cytokine IL8 in monocytic cells. J Med Microbiol 54: 225 – 233.en_US
dc.identifier.citedreferenceLoesche, W.J., Abrams, J., Terpenning, M.S., Bretz, W.A., Dominguez, B.L., Grossman, N.S., et al. ( 1995 ) Dental findings in geriatric populations with diverse medical backgrounds. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 80: 43 – 54.en_US
dc.identifier.citedreferenceLopatin, D.E., Shelburne, C.E., Van Poperin, N., Kowalski, C.J., and Bagramian, R.A. ( 1999 ) Humoral immunity to stress proteins and periodontal disease. J Periodontol 70: 1185 – 1193.en_US
dc.identifier.citedreferenceLupu, F., Heim, D., Bachmann, F., and Kruithof, E.K. ( 1994 ) Expression of LDL receptor-related protein/alpha 2-macroglobulin receptor in human normal and atherosclerotic arteries. Arterioscler Thromb 14: 1438 – 1444.en_US
dc.identifier.citedreferenceMcLaughlin, J.O., Coulter, W.A., Coffey, A., and Burden, D.J. ( 1996 ) The incidence of bacteremia after orthodontic banding. Am J Orthod Dentofacial Orthop 109: 639 – 644.en_US
dc.identifier.citedreferenceMian, B.M., Dinney, C.P., Bermejo, C.E., Sweeney, P., Tellez, C., Yang, X.D., et al. ( 2003 ) Fully human anti-interleukin 8 antibody inhibits tumor growth in orthotopic bladder cancer xenografts via down-regulation of matrix metalloproteases and nuclear factor-kappaB. Clin Cancer Res 9: 3167 – 3175.en_US
dc.identifier.citedreferenceMoseley, P. ( 2000 ) Stress proteins and the immune response. Immunopharmacology 48: 299 – 302.en_US
dc.identifier.citedreferencePeiser, L., Mukhopadhyay, S., and Gordon, S. ( 2002a ) Scavenger receptors in innate immunity. Curr Opin Immunol 14: 123 – 128.en_US
dc.identifier.citedreferencePeiser, L., De Winther, M.P., Makepeace, K., Hollinshead, M., Coull, P., Plested, J., et al. ( 2002b ) The class A macrophage scavenger receptor is a major pattern recognition receptor for Neisseria meningitidis which is independent of lipopolysaccharide and not required for secretory responses. Infect Immun 70: 5346 – 5354.en_US
dc.identifier.citedreferenceRobertson, M.J. ( 2002 ) Role of chemokines in the biology of natural killer cells. J Leukoc Biol 71: 173 – 183.en_US
dc.identifier.citedreferenceSkjolaas, K.A., Burkey, T.E., Dritz, S.S., and Minton, J.E. ( 2006 ) Effects of Salmonella enterica serovars Typhimurium (ST) and Choleraesuis (SC) on chemokine and cytokine expression in swine ileum and jejunal epithelial cells. Vet Immunol Immunopathol 111: 199 – 209.en_US
dc.identifier.citedreferenceSocransky, S.S., and Haffajee, A.D. ( 1992 ) The bacterial etiology of destructive periodontal disease: current concepts. J Periodontol 63: 322 – 331.en_US
dc.identifier.citedreferenceSocransky, S.S., and Haffajee, A.D. ( 1997 ) The nature of periodontal diseases. Ann Periodontol 2: 3 – 10.en_US
dc.identifier.citedreferenceStewart, G.R., and Young, D.B. ( 2004 ) Heat-shock proteins and the host–pathogen interaction during bacterial infection. Curr Opin Immunol 16: 506 – 510.en_US
dc.identifier.citedreferenceSweier, D.G., Combs, A., Shelburne, C.E., Fenno, J.C., and Lopatin, D.E. ( 2003 ) Construction and characterization of a Porphyromonas gingivalis htpG disruption mutant. FEMS Microbiol Lett 225: 101 – 106.en_US
dc.identifier.citedreferenceTsai, C.M. ( 1986 ) The analysis of lipopolysaccharide (endotoxin) in meningococcal polysaccharide vaccines by silver staining following SDS-polyacrylamide gel electrophoresis. J Biol Stand 14: 25 – 33.en_US
dc.identifier.citedreferenceWang, Y., Kelly, C.G., Karttunen, J.T., Whittall, T., Lehner, P.J., Duncan, L., et al. ( 2001 ) CD40 is a cellular receptor mediating mycobacterial heat shock protein 70 stimulation of CC-chemokines. Immunity 15: 971 – 983.en_US
dc.identifier.citedreferenceYuan, J.P., Li, T., Chen, H.B., Li, Z.H., Yang, G.Z., Hu, B.Y., et al. ( 2004 ) Analysis of gene expression profile in gastric cancer cells stimulated with Helicobacter pylori isogenic strains. J Med Microbiol 53: 965 – 974.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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