View Item 

Show simple item record

Pteroylpolyglutamate synthesis by lung- and culture-derived Pneumocystis carinii

dc.contributor.authorHong, Yu-longen_US
dc.contributor.authorBartlett, Marilyn S.en_US
dc.contributor.authorQueener, Sherryen_US
dc.contributor.authorSmith, James W.en_US
dc.contributor.authorShaw, Margareten_US
dc.contributor.authorMeshnick, Steven R.en_US
dc.date.accessioned2010-06-01T18:45:47Z
dc.date.available2010-06-01T18:45:47Z
dc.date.issued1995-12en_US
dc.identifier.citationHong, Yu-long; Bartlett, Marilyn S.; Queener, Sherry; Smith, James W.; Shaw, Margaret; Meshnick, Steven R. (1995). "Pteroylpolyglutamate synthesis by lung- and culture-derived Pneumocystis carinii ." FEMS Microbiology Letters 134(2-3): 251-254. <http://hdl.handle.net/2027.42/71960>en_US
dc.identifier.issn0378-1097en_US
dc.identifier.issn1574-6968en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/71960
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8586276&dopt=citationen_US
dc.description.abstractPneumocystis carinii synthesizes folates de novo from exogenous p -aminobenzoic acid (pABA). Lung-derived organisms take up [ 3 H]pABA in vitro except in the presence of sulfamethoxazole. Supernatants from spinner-flask cultures take up [ 3 H]pABA if they were inoculated with lungs from infected rats, but not if they were inoculated with lungs from uninfected rats. P. carinii folates consist primarily of pteroylpentaglutamates. Plasmodium falciparum , in contrast, contains primarily pteroyltetraglutamates. Culture-derived organisms synthesize folates at a four-fold higher specific activity than lung-derived organisms, possibly because they contain less contaminating lung debris. These data suggest that P. carinii remains metabolically active in culture for at least 4 days.en_US
dc.format.extent349948 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherBlackwell Publishing Ltden_US
dc.rights1995 Federation of European Microbiological Societiesen_US
dc.subject.otherPneumocystis Cariniien_US
dc.subject.otherFolateen_US
dc.subject.otherOpportunistic Infectionen_US
dc.subject.otherFolylpolyglutamateen_US
dc.subject.otherSulfamethoxazoleen_US
dc.titlePteroylpolyglutamate synthesis by lung- and culture-derived Pneumocystis cariniien_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationotherDepartment of Pathology and Laboratory Medicine, Indiana University Medical Center, Indianapolis, IA, USAen_US
dc.identifier.pmid8586276en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/71960/1/j.1574-6968.1995.tb07946.x.pdf
dc.identifier.doi10.1111/j.1574-6968.1995.tb07946.xen_US
dc.identifier.sourceFEMS Microbiology Lettersen_US
dc.identifier.citedreferenceWalker, R.E. and Masur, H. ( 1994 ) In Pneumocystis carinii Pneumonia Current regimes of therapy and prophylaxis in Pneumocystis carinii pneumonia, Walzer, P., Ed ) 2nd edn., pp 439 – 466 Marcel Dekker, New York, NY.en_US
dc.identifier.citedreferenceMcguire, J.J. and Coward, J.K. ( 1984 ) Pteroylpolyglutamates: biosynthesis, degradation, and function 2nd edn. In Folates and Pterins Blakley, R.L. and Benkovic, S.J., Eds ) Vol. I, pp 135 – 190 John Wiley & Sons, New York, NY.en_US
dc.identifier.citedreferenceTrager, W. and Jensen, J.B. ( 1976 ) Human malarial parasite in continuous culture Science, 193, 673 – 675.en_US
dc.identifier.citedreferenceFairfield, A.S., Meshnick, S.R. and Eaton, J.W. ( 1983 ) Malaria parasites adopt host cell Superoxide dismutase Science, 221, 764 – 766.en_US
dc.identifier.citedreferenceIttarat, I., Asawamahasakda, W., Bartlett, M.S., Smith, J.W. and Meshnick, S.R. ( 1995 ) Effects of atovaquone and other inhibitors on Pneumocystis carinii dihydroorotate dehydrogenase Antimicrob. Agents Chemother., 39, 325 – 328.en_US
dc.identifier.citedreferenceLee, C., Bauer, N., Shaw, M., Durkin, M., Bartlett, M.S., Queener, S. and Smith, J.W. ( 1993 ) Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks J. Clin. Microbiol., 31, 1659 – 1662.en_US
dc.identifier.citedreferenceBradford, M. ( 1976 ) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem., 72, 248 – 254.en_US
dc.identifier.citedreferenceShane, B. ( 1986 ) Identification of folylpoly(γ-glutamate) chain length by cleavage to and separation of p -aminobenzoylpoly(γ-glutamates) Methods Enzymol., 122, 323 – 330.en_US
dc.identifier.citedreferenceKovacs, J.A., Allegra, C.J., Beaver, J., Boarman, D., Lweis, M., Parrillo, J.E., Chabner, B. and Masur, H. ( 1989 ) Characterization of de novo folate synthesis in Pneumocystis carinii and Toxoplasma gondii potential for screening therapeutic agents. J. Infect. Dis., 160, 312 – 320.en_US
dc.identifier.citedreferenceComley, J.C.W., Mullin, R.J., Wolfe, L.A., Hanlon, M.J. and Ferone, R. ( 1991 ) Microculture screening assay for primary in vitro evaluation of drugs against Pneumocystis carinii Antimicrob. Agents Chemother., 35, 1965 – 1974.en_US
dc.identifier.citedreferenceMerali, S., Zhang, Y., Sloan, D. and Meshnick, S.R. ( 1990 ) Inhibition of Pneumocystis carinii dihydropteroate synthetase by sulfa drugs Antimicrob. Agents Chemother., 34, 1075 – 1078.en_US
dc.identifier.citedreferenceVoeller, D., Kovacs, J., Andrawis, V., Chu, E., Masur, H. and Allegra, C. ( 1994 ) Interaction of Pneumocystis carinii dihydropteroate synthetase with sulfonamides and diaminophenyl sulfone (dapsone) J. Infect. Dis., 169, 456 – 459.en_US
dc.identifier.citedreferenceHong, Y.-L., Hossler, P.A., Calhoun, D.H. and Meshnick, S.R. ( 1995 ) Inhibition of recombinant Pneumocystis carinii dihydropteroate synthetase by sulfa drugs Antimicrob. Agents Chemother., 39, 1756 – 1763.en_US
dc.identifier.citedreferenceZhang, Y., Merali, S. and Meshnick, S.R. ( 1992 ) p -Aminobenzoic acid transport by normal and Plasmodium falciparum -infected erythrocytes Mol. Biochem. Parasitol., 52, 185 – 194.en_US
dc.identifier.citedreferenceZhang, Y. and Meshnick, S.R. ( 1991 ) Inhibition of the P falciparum dihydropteroate synthetase and of parasite growth in vitro by sulfa drugs Antimicrob. Agents Chemother., 35, 267 – 271.en_US
dc.identifier.citedreferenceSeihub, J. ( 1989 ) Determination of tissue folate composition by affinity chromatography followed by high-pressure ion pair liquid chromatography Anal. Biochem., 182, 84 – 93.en_US
dc.identifier.citedreferenceKrungkrai, J., Webster, H.K. and Yuthavong, Y. ( 1989 ) De novo and salvage biosynthesis of pteroylpentaglutamates in the human malaria parasite, Plasmodium falciparum Mol. Biochem. Parasitol., 32, 25 – 38.en_US
dc.identifier.citedreferenceCossins, E.A. ( 1984 ) Folates in biological material 2nd edn. In Folates and Pterins Blakley, R.L. and Benkovic, S.J., Eds ) Vol. I, pp 1 – 60 John Wiley & Sons, New York, NY.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
j.1574-6968.1995.tb07946.x.pdf
Size:
341.7KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.