Adaptive correction of depth-induced aberrations in multiphoton scanning microscopy using a deformable mirror

Sherman, L.; Ye, J. Y.; Albert, O.; Norris, T. B.

2002-04

Citation: Sherman, L . ; Ye, J. Y . ; Albert, O . ; Norris, T. B . (2002). "Adaptive correction of depth-induced aberrations in multiphoton scanning microscopy using a deformable mirror." Journal of Microscopy 206(1): 65-71. <http://hdl.handle.net/2027.42/72425>

Abstract: We demonstrate adaptive aberration correction for depth-induced spherical aberration in a multiphoton scanning microscope with a micromachined deformable mirror. Correction was made using a genetic learning algorithm with two-photon fluorescence intensity feedback to determine the desired shape for an adaptive mirror. For a 40×/0.6 NA long working distance objective, the axial scanning range was increased from 150 mm to 600 mm.

ISSN: 0022-2720, 1365-2818

DOIs: 10.1046/j.1365-2818.2002.01004.x

PMID: 12000564

URI: http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12000564&dopt=citation

Handle: http://hdl.handle.net/2027.42/72425

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