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LuxO controls luxR expression in Vibrio harveyi : evidence for a common regulatory mechanism in Vibrio

dc.contributor.authorMiyamoto, Carol M.en_US
dc.contributor.authorDunlap, Paul V.en_US
dc.contributor.authorRuby, Edward G.en_US
dc.contributor.authorMeighen, Edward A.en_US
dc.date.accessioned2010-06-01T19:33:49Z
dc.date.available2010-06-01T19:33:49Z
dc.date.issued2003-04en_US
dc.identifier.citationMiyamoto, Carol M.; Dunlap, Paul V.; Ruby, Edward G.; Meighen, Edward A. (2003). "LuxO controls luxR expression in Vibrio harveyi : evidence for a common regulatory mechanism in Vibrio ." Molecular Microbiology 48(2): 537-548. <http://hdl.handle.net/2027.42/72700>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/72700
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12675810&dopt=citationen_US
dc.description.abstractQuorum-sensing control of luminescence in Vibrio harveyi , which involves an indirect autoinducer-mediated phosphorelay signal transduction system, contrasts with the prototypical quorum-sensing system of Vibrio fischeri , in which the autoinducer and the transcriptional activator LuxR directly activate lux operon expression. In V. harveyi , a regulator not homologous to V. fischeri LuxR and also designated LuxR (LuxR vh ), binds specifically to the lux operon promoter region and activates the expression of luminescence. A direct connection has not been identified previously between V. harveyi LuxR vh and the autoinducer-mediated phosphorelay system. Here, we demonstrate by mobility shift assays and measurement of luxR vh mRNA levels with luxO + and luxO – cells that the central response regulator of the V. harveyi phosphorelay system (LuxO) represses the level of LuxR vh . Expression of a luxR vh -bearing plasmid strongly stimulated luminescence of a luxO – mutant but had no effect on luminescence of wild-type luxO + cells, indicating tight regulation of luxR vh by LuxO. Furthermore, luxO null mutants of V. fischeri MJ-1 and two autoinducer mutants, MJ-211 ( luxI – ) and MJ-215 ( luxI – ainS – ), emitted more light and exhibited more elevated levels of litR , a newly identified V. harveyi luxR vh homologue, than their luxO + counterparts. These results suggest that activity of the autoinducer-mediated phosphorelay system is coupled to LuxR vh /LitR control of luminescence through LuxO in V. harveyi and V. fischeri . The presence of homologues of V. harveyi LuxR vh , LuxO and other phosphorelay system proteins in various Vibrio species and the control of LuxR vh and its homologues by LuxO identified here in V. harveyi and V. fischeri and recently in Vibrio cholerae suggest that the luxO–luxR vh couple is a central feature of this quorum-sensing system in members of the genus Vibrio .en_US
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dc.publisherBlackwell Science Ltden_US
dc.rights2003 Blackwell Publishing Ltden_US
dc.titleLuxO controls luxR expression in Vibrio harveyi : evidence for a common regulatory mechanism in Vibrioen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Ecology and Evolutionary Biology, Kraus Natural Science Building, University of Michigan, 830 North University Avenue, Ann Arbor, MI 48109-1048, USA.en_US
dc.contributor.affiliationotherDepartment of Biochemistry, Room 813, McIntyre Medical Sciences Building, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada, H3G 1Y6.en_US
dc.contributor.affiliationotherPacific Biomedical Research Center, University of Hawaii, Manoa, 41 Ahui Street, Honolulu, HI 96813, USA.en_US
dc.identifier.pmid12675810en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/72700/1/j.1365-2958.2003.03453.x.pdf
dc.identifier.doi10.1046/j.1365-2958.2003.03453.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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