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The high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infection

dc.contributor.authorJacobsen, Sandra M.en_US
dc.contributor.authorLane, Mary C.en_US
dc.contributor.authorHarro, Jean M.en_US
dc.contributor.authorShirtliff, Mark E.en_US
dc.contributor.authorMobley, Harry L. T.en_US
dc.date.accessioned2010-06-01T19:35:08Z
dc.date.available2010-06-01T19:35:08Z
dc.date.issued2008-03en_US
dc.identifier.citationJacobsen, Sandra M.; Lane, Mary C.; Harro, Jean M.; Shirtliff, Mark E.; Mobley, Harry L. T. (2008). "The high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infection." FEMS Immunology & Medical Microbiology 52(2): 180-193. <http://hdl.handle.net/2027.42/72722>en_US
dc.identifier.issn0928-8244en_US
dc.identifier.issn1574-695Xen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/72722
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18194341&dopt=citationen_US
dc.description.abstractProteus mirabilis is a ubiquitous bacterium associated with complicated urinary tract infection (UTI). Mutagenesis studies of the wild-type strain HI4320 in the CBA mouse model of ascending UTIs have identified attenuated mutants with transposon insertions in genes encoding the high-affinity phosphate transporter Pst ( pstS , pstA ). The transcription of the pst operon ( pstSCAB-phoU ) and other members of the phosphate regulon of Escherichia coli , including alkaline phosphatase (AP), are regulated by the two-component regulatory system PhoBR and are repressed until times of phosphate starvation. This normal suppression was relieved in pstS :Tn5 and pstA :Tn5 mutants, which constitutively produced AP regardless of growth conditions. No significant growth defects were observed in vitro for the pst mutants during the independent culture or coculture studies in rich broth, phosphate-limiting minimal salts medium, or human urine. Mutants complemented with the complete pst operon repressed AP synthesis in vitro and colonized the mouse bladder in numbers comparable to the wild-type strain HI4320. Therefore, the Pst transport system imparts a significant in vivo advantage to wild-type P. mirabilis that is not required for in vitro growth. Thus, the Pst transporter has satisfied molecular Koch's postulates as a virulence factor in the pathogenesis of urinary tract infection caused by P. mirabilis .en_US
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dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights© 2008 Federation of European Microbiological Societiesen_US
dc.subject.otherProteus Mirabilisen_US
dc.subject.otherUrinary Tract Infectionen_US
dc.subject.otherVirulenceen_US
dc.subject.otherPhosphateen_US
dc.titleThe high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infectionen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA ; anden_US
dc.contributor.affiliationotherDepartment of Microbiology and Immunology, School of Medicine, University of Maryland – Baltimore, Baltimore, MD, USA ;en_US
dc.contributor.affiliationotherDepartment of Biomedical Sciences, Dental School, University of Maryland – Baltimore, Baltimore, MD, USAen_US
dc.identifier.pmid18194341en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/72722/1/j.1574-695X.2007.00358.x.pdf
dc.identifier.doi10.1111/j.1574-695X.2007.00358.xen_US
dc.identifier.sourceFEMS Immunology & Medical Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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