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Cellular localization of a Hsp90 homologue in Porphyromonas gingivalis

dc.contributor.authorLopatin, Dennis E.en_US
dc.contributor.authorJaramillo, Eduardoen_US
dc.contributor.authorEdwards, Chris A.en_US
dc.contributor.authorPoperin, Nealen_US
dc.contributor.authorCombs, Allisonen_US
dc.contributor.authorShelburne, Charles E.en_US
dc.date.accessioned2010-06-01T19:43:22Z
dc.date.available2010-06-01T19:43:22Z
dc.date.issued1999-12en_US
dc.identifier.citationLopatin, Dennis E.; Jaramillo, Eduardo; Edwards, Chris A.; Poperin, Neal; Combs, Allison; Shelburne, Charles E. (1999). "Cellular localization of a Hsp90 homologue in Porphyromonas gingivalis ." FEMS Microbiology Letters 181(1): 9-16. <http://hdl.handle.net/2027.42/72856>en_US
dc.identifier.issn0378-1097en_US
dc.identifier.issn1574-6968en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/72856
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10564783&dopt=citationen_US
dc.description.abstractWe previously reported an association between elevated serum antibody titers to the 90-kDa human heat shock protein (Hsp90), periodontal health and colonization by Porphyromonas gingivalis . In this study, we examined the cellular localization of the Hsp90 homologue of P. gingivalis . Cultures of P. gingivalis were heat-stressed (45°C) and examined for localization of the Hsp90 homologue. Heat stress induced a 4–5-fold increase in anti-Hsp90 antibody reactivity over that of the unstressed controls. Western blot analysis revealed two bands (44 and 68 kDa) that reacted with anti-Hsp90 antibodies. The 68-kDa band was heat-inducible, while the 44-kDa band was not. Immunogold staining revealed that the Hsp90 homologue localized principally to the membrane and extracellular vesicles. Subcellular fractionation confirmed that the Hsp90 homologue was primarily membrane-associated.en_US
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dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights1999 Federation of European Microbiological Societiesen_US
dc.subject.otherHeat Shocken_US
dc.subject.otherExtracellular Vesicleen_US
dc.subject.other90-kDa Human Heat Shock Proteinen_US
dc.subject.otherPorphyromonas Gingivalisen_US
dc.titleCellular localization of a Hsp90 homologue in Porphyromonas gingivalisen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biologic and Materials Science, University of Michigan School of Dentistry, 1011 North University Ave, Campus Box 1078, Ann Arbor, MI 48109-1078, USAen_US
dc.contributor.affiliationotherBioMaterials Technology Center, 3M, St. Paul, MN, USAen_US
dc.identifier.pmid10564783en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/72856/1/j.1574-6968.1999.tb08820.x.pdf
dc.identifier.doi10.1111/j.1574-6968.1999.tb08820.xen_US
dc.identifier.sourceFEMS Microbiology Lettersen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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