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Localization of protein kinase C ε to macrophage vacuoles perforated by Listeria monocytogenes cytolysin

dc.contributor.authorShaughnessy, Lee M.en_US
dc.contributor.authorLipp, Peteren_US
dc.contributor.authorLee, Kyung-Dallen_US
dc.contributor.authorSwanson, Joel A.en_US
dc.date.accessioned2010-06-01T20:08:35Z
dc.date.available2010-06-01T20:08:35Z
dc.date.issued2007-07en_US
dc.identifier.citationShaughnessy, Lee M.; Lipp, Peter; Lee, Kyung-Dall; Swanson, Joel A. (2007). "Localization of protein kinase C ε to macrophage vacuoles perforated by Listeria monocytogenes cytolysin." Cellular Microbiology 9(7): 1695-1704. <http://hdl.handle.net/2027.42/73267>en_US
dc.identifier.issn1462-5814en_US
dc.identifier.issn1462-5822en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/73267
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17346313&dopt=citationen_US
dc.description.abstractThree proteins secreted by Listeria monocytogenes facilitate escape from macrophage vacuoles: the cholesterol-dependent cytolysin listeriolysin O (LLO), a phosphoinositide-specific phospholipase C (PI-PLC) and a broad-range phospholipase C (PC-PLC). LLO and PI-PLC can activate several members of the protein kinase C (PKC) family during infection. PKCε is a novel PKC that contributes to macrophage activation, defence against bacterial infection, and phagocytosis; however, a role for PKCε in Lm infections has not been described. To study PKCε dynamics, PKCε-YFP chimeras were visualized in macrophages during Lm infection. PKCε-YFP was recruited to forming vacuoles during macrophage phagocytosis of Lm and again later to fully formed Lm vacuoles. The PKCε-YFP localization to the fully formed Lm vacuole was LLO-dependent but independent of PI-PLC or PC-PLC. PKCε-YFP recruitment often followed LLO perforation of the membrane, as indicated by localization of PKCε-YFP to Lm vacuoles after they released small fluorescent dyes into the cytoplasm. PKCε-YFP recruitment to vesicles also followed phagocytosis of LLO-containing liposomes or osmotic lysis of endocytic vesicles, indicating that vacuole perforation by LLO was the chief cause of the PKCε response. These studies implicate PKCε in a cellular mechanism for recognizing damaged membranous organelles, including the disrupted vacuoles created when Lm escapes into cytoplasm.en_US
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dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights© 2007 The Authors; Journal compilation © 2007 Blackwell Publishing Ltden_US
dc.titleLocalization of protein kinase C ε to macrophage vacuoles perforated by Listeria monocytogenes cytolysinen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.en_US
dc.contributor.affiliationumDepartment of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.en_US
dc.contributor.affiliationotherInstitute for Molecular Cell Biology, Saarland University, Homburg, Germany.en_US
dc.identifier.pmid17346313en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/73267/1/j.1462-5822.2007.00903.x.pdf
dc.identifier.doi10.1111/j.1462-5822.2007.00903.xen_US
dc.identifier.sourceCellular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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