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SpoT governs Legionella pneumophila differentiation in host macrophages

dc.contributor.authorDalebroux, Zachary D.en_US
dc.contributor.authorEdwards, Rachel L.en_US
dc.contributor.authorSwanson, Michele S.en_US
dc.date.accessioned2010-06-01T20:11:11Z
dc.date.available2010-06-01T20:11:11Z
dc.date.issued2009-02en_US
dc.identifier.citationDalebroux, Zachary D.; Edwards, Rachel L.; Swanson, Michele S. (2009). "SpoT governs Legionella pneumophila differentiation in host macrophages." Molecular Microbiology 71(3): 640-658. <http://hdl.handle.net/2027.42/73309>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/73309
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19040633&dopt=citationen_US
dc.description.abstractDuring its life cycle, Legionella pneumophila alternates between a replicative and a transmissive state. To determine their contributions to L. pneumophila differentiation, the two ppGpp synthetases, RelA and SpoT, were disrupted. Synthesis of ppGpp was required for transmission, as relA spoT mutants were killed during entry to and exit from macrophages. RelA, which senses amino acid starvation induced by serine hydroxamate, is dispensable in macrophages, as relA mutants spread efficiently. SpoT monitors fatty acid biosynthesis (FAB), since following cerulenin treatment, wild-type and relA strains expressed the flaA transmissive gene, but relA spoT mutants did not. As in Escherichia coli , the SpoT response to FAB perturbation likely required an interaction with acyl-carrier protein (ACP), as judged by the failure of the spoT-A413E allele to rescue transmissive trait expression of relA spoT bacteria. Furthermore, SpoT was essential for transmission between macrophages, since secondary infections by relA spoT mutants were restored by induction of spoT , but not relA . To resume replication, ppGpp must be degraded, as mutants lacking spoT hydrolase activity failed to convert from the transmissive to the replicative phase in either bacteriological medium or macrophages. Thus, L. pneumophila requires SpoT to monitor FAB and to alternate between replication and transmission in macrophages.en_US
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dc.publisherBlackwell Publishing Ltden_US
dc.rightsJournal compilation © 2009 Blackwell Publishingen_US
dc.titleSpoT governs Legionella pneumophila differentiation in host macrophagesen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumCellular and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, MI, USA.en_US
dc.contributor.affiliationotherDepartment of Microbiology & Immunology anden_US
dc.identifier.pmid19040633en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/73309/1/j.1365-2958.2008.06555.x.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/73309/2/MMI_6555_sm_Figure_S1.pdf
dc.identifier.doi10.1111/j.1365-2958.2008.06555.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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