Expression and role of the universal stress protein, UspA, of Escherichia coli during growth arrest
dc.contributor.author | Nyström, Thomas | en_US |
dc.contributor.author | Neidhardt, Frederick C. | en_US |
dc.date.accessioned | 2010-06-01T20:39:06Z | |
dc.date.available | 2010-06-01T20:39:06Z | |
dc.date.issued | 1994-02 | en_US |
dc.identifier.citation | NystrÖm, Thomas; Neidhardt, Frederick C. (1994). "Expression and role of the universal stress protein, UspA, of Escherichia coli during growth arrest." Molecular Microbiology 11(3): 537-544. <http://hdl.handle.net/2027.42/73757> | en_US |
dc.identifier.issn | 0950-382X | en_US |
dc.identifier.issn | 1365-2958 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/73757 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8152377&dopt=citation | en_US |
dc.description.abstract | The synthesis of the small, cytoplasmic protein UspA universal stress protein A) of Escherichia coli is induced as soon as the cell growth rate falls below the maximal growth rate supported by the medium, regardless of the condition inhibiting growth. The increase in UspA synthesis appears to be the result of Induction of the monocistronic uspA gene. Induction of this gene during a heat-shock treatment is demonstrated to be the result of transcriptional activation of Σ 70 -dependent promoter which has previously been shown to be activated also during carbon starvation-induced growth arrest. Mutant cells lacking UspA grow at rates indistinguisible from the isogenic parent at different temperatures and in the presence of different growth inhibitors but are impaired in their ability to survive prolonged periods of complete growth inhibition caused by a variety of diverse stresses, including CdCl 2 , H 2 O 2 , DNP, CCCP exposure, and osmotic shock. Moreover, the uspA mutation results in an increased sensitivity of cells to carbon-source starvation (i.e. glucose, glycerol or succinate depletion). Also, the mutation causes a marked alteration in the timing of starvation protein expression but protein expression during steady-state growth appears to be normal. The results presented have prompted us to postulate that UspA may have a general protective function related to the growth arrest state. | en_US |
dc.format.extent | 3104398 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1994 Blackwell Publishing | en_US |
dc.title | Expression and role of the universal stress protein, UspA, of Escherichia coli during growth arrest | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, 6643 Medical Science Building II, Ann Arbor, Michigan 48109-0620, USA. | en_US |
dc.identifier.pmid | 8152377 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/73757/1/j.1365-2958.1994.tb00334.x.pdf | |
dc.identifier.doi | 10.1111/j.1365-2958.1994.tb00334.x | en_US |
dc.identifier.source | Molecular Microbiology | en_US |
dc.identifier.citedreference | Godell, W., and Tomasz, A. ( 1980 ) Alteration of Escherichia coli murein during amino acid starvation. J Bacteriol 14: 1009 – 1016. | en_US |
dc.identifier.citedreference | Hengge-Aronis, R. ( 1993 ) The role of rpoS in early stationary phase gene regulation in Escherichia coli K12. In Starvation in bacteria. Kjelleberg, S. ( ed. ). New York: Plenum Press, pp. 171 – 200. | en_US |
dc.identifier.citedreference | Hengge-Aronis, R., Klein, W., Lange, R., Rimmele, M., and Boos, W. ( 1991 ) Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary phase thermotoleranee in Escherichia coli. J Bacteriol 173: 7918 – 7924. | en_US |
dc.identifier.citedreference | Jenkins, D.E., Schultz, J.E., and Matin, A. ( 1988 ) Starvation-induced cross protection against heat or H 2 O 2 challenge In Escherichia coli. J Bacteriol 170: 3910 – 3914. | en_US |
dc.identifier.citedreference | Jenkins, D.E., Chaisson, S.A., and Matin, A. ( 1990 ) Starvation induced cross protection against osmotic challenge in Escherichia coli. J Bacteriol 172: 2779 – 2781. | en_US |
dc.identifier.citedreference | Lange, R., and Hengge-Aronis, R. ( 1991 ) Identification of a central regulator of stationary phase gene expression in Escherichia coli. Mol Microbiol 5: 49 – 59. | en_US |
dc.identifier.citedreference | Li, C., and Clarke, S. ( 1992 ) A protein methyltransferase specific for altered aspartyl residues is important in Escherichia coli stationary-phase survival and heat-shock resistance. Proc Natl Acad Sci USA 89: 9885 – 9889. | en_US |
dc.identifier.citedreference | McCann, M.P., Kidwell, J.P., and Matin, A. ( 1991 ) The putative Σ factor KatF has a central role in development of of starvation-mediated general resistance in Escherichia coli. J Bacteriol 173: 4188 – 4194. | en_US |
dc.identifier.citedreference | Miller, J. ( 1972 ) Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press. | en_US |
dc.identifier.citedreference | Mostellar, R.O. ( 1978 ) Evidence that glucose starvation-sensitive mutants are altered in the relB locus. J Bacteriol 133: 1034 – 1037. | en_US |
dc.identifier.citedreference | Mosteller, R.D., and Kwan, S.F. ( 1976 ) Isolation of relaxed-control mutants of Escherichia coli K-12 which are sensitive to glucose starvation. Biochem Biophys Res Commun 69: 325 – 332. | en_US |
dc.identifier.citedreference | Mulvey, M.R., and Loewen, P.C. ( 1989 ) Nucleotide sequence of katF of Escherichia coli suggests KatF protein is a novel Sigma transcription factor. Nucl Acids Res 17: 9979 – 9991. | en_US |
dc.identifier.citedreference | Mulvey, M.R., Switala, J., Borys, A., and Loewen, P.C. ( 1990 ) Regulation of transcription of katE and katF in Escherichia coli. J Bacteriol 172: 6713 – 6720. | en_US |
dc.identifier.citedreference | Neidhardt, F.C., Bloch, P.L., and Smith, D.F. ( 1974 ) Culture medium for enterobacteria. J Bacteriol 119: 736 – 747. | en_US |
dc.identifier.citedreference | NystrÖm, T., and Neidhardt, F.C. ( 1992 ) Cloning, mapping and nucleotide sequencing of a gene encoding a universal stress protein in Escherichia coli. Mol Microbiol 6: 3187 – 3198. | en_US |
dc.identifier.citedreference | NystrÖm, T., and Neidhardt, F.C. ( 1993 ) Isolation and properties of a mutant of Escherichia coli with an insertional inactivation of the uspA gene, which encodes a universal stress protein. J Bacteriol 175: 3949 – 3956. | en_US |
dc.identifier.citedreference | NystrÖm, T., Olsson, R.M., and Kjelleberg, S. ( 1992 ) Survival, stress resistance, and alteration in protein expression in the marine Vibrio sp. strain S14 during starvation for different individual nutrients. Appl Environ Microbiol 58: 55 – 65. | en_US |
dc.identifier.citedreference | O'Farrell, P.H. ( 1975 ) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250: 4007 – 4021. | en_US |
dc.identifier.citedreference | Pedersen, S., Reeh, S.V., Parker, J., Watson, R.J., Friesen, J.D., and Fiil, N.P. ( 1976 ) Analysis of the proteins synthesized in ultraviolet light-irradiated Escherichia coli following infection with the bacteriophage Λ drif d 18 and Λdfus-3. Mol Gen Genet 144: 339 – 344. | en_US |
dc.identifier.citedreference | Sak, B.D., Eisenstark, A., and Touati, D. ( 1989 ) Exonuclease III and the catalase hydroperoxidase II in Escherichia coli are both regulated by the katF product. Proc Natl Acad Sci USA 86: 3271 – 3275. | en_US |
dc.identifier.citedreference | Sambrook, J., Fritsch, E.F., and Maniatis, T. ( 1989 ) Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press. | en_US |
dc.identifier.citedreference | Sammartano, L.J., Tuveson, R.W., and Davenport, R. ( 1986 ) Control of sensitivity to inactivation by H 2 O 2 and broad-spectrum near-UV radiation by Escherichia coli katF locus. J Bacteriol 168: 13 – 21. | en_US |
dc.identifier.citedreference | Smith, M.W., and Neidhardt, F.C. ( 1983 ) Proteins induced by anaerobiosis in Escherichia coli. J Bacteriol 154: 336 – 343. | en_US |
dc.identifier.citedreference | Spence, J., Cegielska, A., and Georgopoulos, C. ( 1990 ) Role of Escherichia coli heat shock proteins DnaK and HtpG (C62.5) in response to nutritional deprivation. J Bacteriol 172: 7157 – 7166. | en_US |
dc.identifier.citedreference | Tanaka, K., Takayanagi, Y., Fujita, N., Ishihama, A., and Takahashi, H. ( 1993 ) Heterogeneity of the principal sigma factor in Escherichia coli: the rpoS gene product, sigma-38, is a second principal sigma factor of RNA polymerase in stationary-phase Escherichia coli. Proc Natl Acad Sci USA 90, 3511 – 3515. | en_US |
dc.identifier.citedreference | Tormo, A., Almiron, M., and Kolter, R. ( 1990 ) surA, an Escherichia coli gene essential for survival in stationary phase. J Bacteriol 172: 4339 – 4347. | en_US |
dc.identifier.citedreference | Tuomanen, E., Markiewicz, Z., and Tomasz, A. ( 1988 ) Autolysis-resistant petidoglycan of anomalous composition in amino acid starved Escherichia coli. J Bacteriol 170: 1373 – 1376. | en_US |
dc.identifier.citedreference | VanBogslen, R.A., and Neidhardt, F.C. ( 1990 ) Ribosomes as sensors of heat and cold shock in Escherichia coli. Proc Natl Acad Sci USA 87: 5589 – 5593. | en_US |
dc.identifier.citedreference | VanBogelen, R.A., Kelly, P.M., and Neidhardt, F.C. ( 1987 ) Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli. J Bacteriol 169: 26 – 32. | en_US |
dc.identifier.citedreference | VanBogelen, R.A., Hutton, M.E., and Neidhardt, F.C. ( 1990 ) Gene-protein database of Escherichia coli K-12: edition 3. Electrophoresis 11: 1131 – 1166. | en_US |
dc.identifier.citedreference | Wanner, B.L., Kodaira, R., and Neidhardt, F.C. ( 1977 ) Physiological regulation of a decontrolled lac operon. J Bacteriol 130: 212 – 222. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.