Expression, but lack of calcium mobilization by high-affinity IgE Fcε receptor I on human epidermal and dermal Langerhans cells
dc.contributor.author | Shibaki, A. | en_US |
dc.contributor.author | Ohkawara, A. | en_US |
dc.contributor.author | Shimada, S. | en_US |
dc.contributor.author | Ra, C. | en_US |
dc.contributor.author | Aiba, S. | en_US |
dc.contributor.author | Cooper, K. D. | en_US |
dc.date.accessioned | 2010-06-01T20:39:58Z | |
dc.date.available | 2010-06-01T20:39:58Z | |
dc.date.issued | 1996-10 | en_US |
dc.identifier.citation | Shibaki, A.; Ohkawara, A.; Shimada, S.; Ra, C.; Aiba, S.; Cooper, K. D. (1996). "Expression, but lack of calcium mobilization by high-affinity IgE Fcε receptor I on human epidermal and dermal Langerhans cells." Experimental Dermatology 5(5): 272-278. <http://hdl.handle.net/2027.42/73771> | en_US |
dc.identifier.issn | 0906-6705 | en_US |
dc.identifier.issn | 1600-0625 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/73771 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8981026&dopt=citation | en_US |
dc.description.abstract | : In atopic dermatitis (AD) patients, IgE molecules are demonstrated on the surface of Langerhans cells (LC). FcεRI molecules, which are present on the surface of LC in AD patients as well as normal individuals, are responsible for this binding. In this study, we have investigated phenotypic and functional characteristics of FcεRI on epidermal and dermal cell populations. Epidermal and dermal cell suspensions were prepared enzymatically with dispase followed by either trypsin or collagenase treatment, respectively. Peripheral blood basophils were negatively selected by excluding other leukocytes with surface marker staining. Consistent with previous reports, both peripheral blood basophils and epidermal LC were positively stained with anti FcεRI monoclonal antibody. In addition, an FcεRI positive population was demon-strated among dermal HLA-DR positive cells. These cells express significant amounts of HLA-DR molecules (DR Hi ) and co-express CD la molecules, which identifies them as LC-like dendritie APC of the dermis. No other FcεRI positive population was found in the other dermal DR Mid or DR populations, except for a minor DR lo population, presumably mast cells. To analyze whether these FcεRI molecules are signal transducing for LC, intracellular calcium mobilization after crosslinking of FcεRI was measured with How cytometry. Following crosslinking, peripheral blood basophils clearly increased intracellular calcium. On the other hand, neither normal epidermal LC nor dermal DR Hi CD Ia + cells changed their intracellular calcium level after FcεRI crosslinking. These data indicate that normal epidermal and dermal LC, but not basophils, are resistant to calcium flux following FcεRI engagement. | en_US |
dc.format.extent | 6298873 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | Munksgaard 1996 | en_US |
dc.subject.other | Langerhans Cells | en_US |
dc.subject.other | FcεRI | en_US |
dc.subject.other | Calcium | en_US |
dc.subject.other | Signal Transduction | en_US |
dc.title | Expression, but lack of calcium mobilization by high-affinity IgE Fcε receptor I on human epidermal and dermal Langerhans cells | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dentistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Immunodermatology Unit, University of Michigan, Ann Arbor, USA | en_US |
dc.contributor.affiliationother | Department of Dermatology, Hokkaido University School of Medicine, Sapporo, Japan | en_US |
dc.contributor.affiliationother | Department of Dermatology, Yamanashi Medical University, Kofu, Japan | en_US |
dc.contributor.affiliationother | Department of Immunology, Juntendo University, Tokyo, Japan | en_US |
dc.contributor.affiliationother | Department of Dermatology, Tohoku University, Sendai, Japan | en_US |
dc.contributor.affiliationother | Department of Dermatology, University Hospitals of Cleveland, Case Western Reserve University, and VA Hospital, Cleveland, USA | en_US |
dc.identifier.pmid | 8981026 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/73771/1/j.1600-0625.1996.tb00129.x.pdf | |
dc.identifier.doi | 10.1111/j.1600-0625.1996.tb00129.x | en_US |
dc.identifier.source | Experimental Dermatology | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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