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Helicobacter pylori Outer Membrane Protein 18 ( Hp1125 ) Induces Dendritic Cell Maturation and Function

dc.contributor.authorRathinavelu, Sivaprakashen_US
dc.contributor.authorKao, John Y.en_US
dc.contributor.authorZavros, Yanaen_US
dc.contributor.authorMerchant, Juanita L.en_US
dc.date.accessioned2010-06-01T20:46:56Z
dc.date.available2010-06-01T20:46:56Z
dc.date.issued2005-10en_US
dc.identifier.citationRathinavelu, Sivaprakash; Kao, John Y.; Zavros, Yana; Merchant, Juanita L. (2005). " Helicobacter pylori Outer Membrane Protein 18 ( Hp1125 ) Induces Dendritic Cell Maturation and Function." Helicobacter 10(5): 424-432. <http://hdl.handle.net/2027.42/73882>en_US
dc.identifier.issn1083-4389en_US
dc.identifier.issn1523-5378en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/73882
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16181353&dopt=citationen_US
dc.description.abstractBackground.  Dendritic cells (DCs) are potent antigen-presenting cells that initiate T-cell responses. A robust adaptive Th1 immune response is crucial to an adaptive (Th2) immune response necessary for vaccine-induced protective immunity against Helicobacter pylori. It has been shown that several outer membrane proteins (Omps) induce a robust antibody response. However, it is also known that the antibodies generated are not protective. Moreover there is great variation in the recognition of high molecular weight H. pylori proteins by sera from infected patients. In contrast to the high molecular weight proteins, serologic responses to small molecular weight proteins provide assessment of current infection with H. pylori and also of its eradication. Aim.  The goal of the study was to analyze the activation of the immune response by a specific low molecular weight Omp that is universally expressed by all H. pylori strains. Therefore, we studied interaction of H. pylori Omp18 with DCs. Methods.  Activation of murine bone marrow-derived DCs and production of cytokines by Omp18 was assessed by fluorescence-activated cell sorter (FACS) for costimulatory markers and ELISA, respectively. The ability of Omp18 stimulated DCs to induce lymphocyte proliferation was measured in a mixed leukocyte reaction. Results.  Omp18 induced higher expression of the B7 (CD80 and CD86) costimulatory molecule after 18 hours indicating processing and presentation of the antigen on the surface by bone marrow-derived DCs. The maturing DCs also secreted significant levels of IL-12, but was 4-fold less than that stimulated by whole bacteria. Omp18-primed DCs induced proliferation and release of IFNγ by syngeneic splenocytes. Conclusion.  We concluded that Omp18 is capable of activating DCs initiating a Th1 immune response.en_US
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dc.format.extent3109 bytes
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dc.publisherBlackwell Science Ltden_US
dc.rights© 2005 Blackwell Publishing Ltden_US
dc.subject.otherOmpsen_US
dc.subject.otherTh1en_US
dc.subject.otherCD80en_US
dc.subject.otherCD86en_US
dc.subject.otherIL-12en_US
dc.titleHelicobacter pylori Outer Membrane Protein 18 ( Hp1125 ) Induces Dendritic Cell Maturation and Functionen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelInternal Medicine and Specialtiesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumMolecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationotherDepartments of Internal Medicine anden_US
dc.identifier.pmid16181353en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/73882/1/j.1523-5378.2005.00350.x.pdf
dc.identifier.doi10.1111/j.1523-5378.2005.00350.xen_US
dc.identifier.sourceHelicobacteren_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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