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Anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescence

dc.contributor.authorAssed, S.en_US
dc.contributor.authorIto, I. Y.en_US
dc.contributor.authorLeonardo, M. R.en_US
dc.contributor.authorSilva, L. A. B.en_US
dc.contributor.authorLopatin, Dennis E.en_US
dc.date.accessioned2010-06-01T21:10:59Z
dc.date.available2010-06-01T21:10:59Z
dc.date.issued1996-04en_US
dc.identifier.citationAssed, S.; Ito, I.Y.; Leonardo, M.R.; Silva, L.A.B.; Lopatin, D.E. (1996). "Anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescence." Dental Traumatology 12(2): 66-69. <http://hdl.handle.net/2027.42/74264>en_US
dc.identifier.issn1600-4469en_US
dc.identifier.issn1600-9657en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/74264
dc.description.abstract– Aiming to assess the presence of selected anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis, 25 central and lateral upper incisors presenting with radiographic evidence of chronic apical periodontitis were studied. I he pulp chamber was opened under aseptic conditions and samples of die root canal content were collected with sterile absorbent paper points, which were placed and dispersed in lest lubes containing reduced transport medium (RTF)Aliquots were dried on glass slides and stained by indirect immunofluorescence for detection of. Actinomyces viscosus, Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia. The results showed a positive indirect immunofluorescence reaction in 24 of the 25 samples. Fourteen were positive for die specie Actinomyces viscous , 12 for Prevotella intermedia , 10 for Fusobacterium nucleatum and 4 for Porphyromonas gingivalis. A semiquantitative assay was easily implemented for assessment of degree of infection by the organisms in individual cases.en_US
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dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rightsMunksgaard 1996en_US
dc.subject.otherApical Periodontitisen_US
dc.subject.otherIndirect Immunofluorescenceen_US
dc.subject.otherAnaerobic Microorganismsen_US
dc.titleAnaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescenceen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelDentistryen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pediatric Dentistry, Ribeirao Preto School of Pharmacy, USP, University of Michigan, Ann Arbor. USA.en_US
dc.contributor.affiliationumDepartment of Health Sciences, Ribeirao Preto School of Pharmacy, USP, University of Michigan, Ann Arbor. USA.en_US
dc.contributor.affiliationumDepartment of Endodontics, Araraquara School of Dentistry, UNESP, University of Michigan, Ann Arbor. USA.en_US
dc.contributor.affiliationumDepartment of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor. USA.en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/74264/1/j.1600-9657.1996.tb00099.x.pdf
dc.identifier.doi10.1111/j.1600-9657.1996.tb00099.xen_US
dc.identifier.sourceDental Traumatologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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