Variable nuclear markers for Melanoplus oregonensis identified from the screening of a genomic library

Abstract

We report the isolation of seven single-copy nuclear loci from the montane grasshopper Melanoplus oregonensis . With an average length of approximately 930 bp and between five and 67 variable sites, these loci are useful for population genetic analyses within M. oregonensis . Amplification of nuclear loci in additional species of western Melanoplus grasshoppers suggests that they will also be useful for a variety of population genetic, phylogeographic and phylogenetic studies.