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Molecular Epidemiology of Cryptosporidiosis in Children in Malawi

dc.contributor.authorPeng, Michael M.en_US
dc.contributor.authorMeshnick, Steve R.en_US
dc.contributor.authorCunliffe, Nigel A.en_US
dc.contributor.authorThindwa, Benson D. M.en_US
dc.contributor.authorHart, C. Anthonyen_US
dc.contributor.authorBroadhead, Robin L.en_US
dc.contributor.authorXiao, Lihuaen_US
dc.date.accessioned2010-06-01T21:18:38Z
dc.date.available2010-06-01T21:18:38Z
dc.date.issued2003-07en_US
dc.identifier.citationPENG, MICHAEL M.; MESHNICK, STEVE R.; CUNLIFFE, NIGEL A.; THINDWA, BENSON D.M.; HART, C. ANTHONY; BROADHEAD, ROBIN L.; XIAO, LIHUA (2003). "Molecular Epidemiology of Cryptosporidiosis in Children in Malawi." Journal of Eukaryotic Microbiology 50(): 557-559. <http://hdl.handle.net/2027.42/74380>en_US
dc.identifier.issn1066-5234en_US
dc.identifier.issn1550-7408en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/74380
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=14736161&dopt=citationen_US
dc.description.abstractFew studies have examined the molecular epidemiology of cryptosporidiosis in developing countries. In this study, DNA of 69 microscopy-positive human fecal samples collected from Malawi were examined by multilocus genetic analyses. From 43, 27 and 28 of the samples, the SSU rRNA, 70 kDa heat shock protein (HSP70) and 60 kDa glycoprotein (GP60) genes, respectively, were successfully PCR-amplified. Restriction analysis of the SSU PCR products showed that 41 of the 43 PCR-positive samples had C. hominis and 2 had C. parvum. Sequence analysis of the HSP70 and GP60 gene contirmed the species identification by SSU rRNA PCR-RFLP analysis, but also revealed high intraspecific variations. Altogether, six HSP70 subtypes and six GP60 subtypes (belonging to lour subtype alleles) of C. hominis were found. Linkage diseyuilibrum analysis of the two genetic loci showed possible intraspecitic recombination. Thus, cryptosporidiosis in the study area was largely caused by anthroponotic transmission. The high intraspecitic variation and existence of genetic recombination were probably results of high transmission of cryptosporidiosis in this area.en_US
dc.format.extent367563 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights2003 by the Society of Protozoologistsen_US
dc.titleMolecular Epidemiology of Cryptosporidiosis in Children in Malawien_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumUniversity of Michigan, Ann Arbor, MIen_US
dc.contributor.affiliationotherCenters for Disease Control and Prevention, Atlanta, GAen_US
dc.contributor.affiliationotherUniversity of North Carolina, Chapel Hill, NCen_US
dc.contributor.affiliationotherWellcome Trust Research Laboratories, Blantyre, Malawien_US
dc.contributor.affiliationotherUniversity of Liverpool, Liverpool, UKen_US
dc.contributor.affiliationotherUniversity of Malawi, Blantyre, Malawien_US
dc.identifier.pmid14736161en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/74380/1/j.1550-7408.2003.tb00628.x.pdf
dc.identifier.doi10.1111/j.1550-7408.2003.tb00628.xen_US
dc.identifier.sourceJournal of Eukaryotic Microbiologyen_US
dc.identifier.citedreferenceAlves, M., Xiao, L., Sulaiman, I., Lal, A.A., Matos, O. & Antunes, F. 2003. Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. J. Clin. Microbiol., 41: 2144 – 2747.en_US
dc.identifier.citedreferenceBern, C., Hernandez, B., Lopez, M.B., Arrowood, M.J., De Merida, A.M. & Klein, R.E. 2000. The contrasting epidemiology of Cyclospora and Cryptosporidium among outpatients in Guatemala. Am.J. Trop. Med. Hyg., 63: 231 – 235.en_US
dc.identifier.citedreferenceBhattacharya, M.K., Teka, T., Faruque, A.S. & Fuchs, G.J. 1997. Cryptosporidium infection in children in urban Bangladesh. J. Trop. Pediatrics, 43: 282 – 286.en_US
dc.identifier.citedreferenceCunliffe, N.A., Gondwe, J.S., Kirkwood, C.D., Graham, S.M., Nhlane, N.M., Thindwa, B.D.M., Dove, W., Broadhead, R.L., Molyneux, M.E. & Hart, C.A. 2001. Effect of concomitant HIV infection on presentation and outcome of rotavirus gastroenteritis in Malawian children. Lancet, 358: 550 – 555.en_US
dc.identifier.citedreferenceGlaberman, S., Moore, J.E., Lowery, C.J., Chalmers, R.M., Sulaiman, I., Elwin, K., Rooney, P.J., Millar, B.C., Dooley, J.S.L.I.A.A. & Xiao, L. 2002. Three drinking-water–associated cryptosporidiosis outbreaks, Northern Ireland. Emerg. Infect. Dis., 8: 631 – 633.en_US
dc.identifier.citedreferenceIqbal, J., Hira, P.R., Al-Ali, F. & Philip, R. 2001. Cryptosporidiosis in Kuwaiti children: seasonality and endemicity. Clin. Microbiol. Infect., 7: 261 – 266.en_US
dc.identifier.citedreferenceJavier Enriquez, F., Avila, C.R., Ignacio Santos, J., Tanaka-Kido, J., Vallejo, O. & Sterling, C.R. 1997. Cryptosporidium infections in Mexican children: clinical, nutritional, enteropathogenic, and diagnostic evaluations. Am. J. Trop. Med. Hyg., 56: 254 – 257.en_US
dc.identifier.citedreferenceLeav, B.A., Mackay, M.R., Anyanwu, A., O'Connor, R.M., Cevallos, A.M., Kindra, G., Rollins, N.C., Bennish, M.L., Nelson, R.G. & Ward, H.D. 2002. Analysis of sequence diversity at the highly polymorphic Cpgp40/15 locus among Cryptosporidium isolates from human immunodeficiency virus-infected children in South Africa. Infect. Immun., 70: 3881 – 3890.en_US
dc.identifier.citedreferenceMallon, M., MacLeod, A., Wading, J., Smith, H., Reilly, B. & Tail, A. 2003. Population structures and the role of genetic exchange in the zoonotic pathogen Cryptosporidium parvum. J. Mol. Evol., 56: 407417.en_US
dc.identifier.citedreferenceNewman, R.D., Sears, C.L., Moore, S. R., Nataro, J.P., Wuhib, T., Agnew, D.A., Guerrant, R.L. & Lima, A.A.M. 1999. Longitudinal study of Cryptosporidium infection in children in northeastern Brazil. J. Infect. Dis., 180: 167 – 175.en_US
dc.identifier.citedreferencePeng, M.M., Matos, O., Gatei, W., Das, P., Stantic-Pavlinic, M., Bern, C., Sulaiman, I.M., Glaberman, S.L.I.A.A. & Xiao, L. 2001 A comparison of Cryptosporidium subgenotypes from several geographic regions. J. Eukuryot. Microbiol., 48: 288 – 316.en_US
dc.identifier.citedreferenceStrong, W.B., Gut, J. & Nelson, R.G. 2000. Cloning and sequence analysis of a highly polymorphic Cryptosporidium parvum gene encoding a 60-kilodalton glycoprotein and characterization of its 15- and 45-kilodalton zoite surface antigen products. Infect. Immun., 68: 4117 – 34.en_US
dc.identifier.citedreferenceSulaiman, I.M.L.I.A.A. & Xiao, L. 2001. A population genetic study of the Cryptosporidium parvum human genotype parasites. J. Eukaryot. Microbiol., 48: 243 – 278.en_US
dc.identifier.citedreferenceXiao, L., Bern, C., Limor, J., Sulaiman, I., Roberts, J., Checkley, W., Cabrera, L., Gilman, R.H. & Lal, A.A. 2001. Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru. J. Infect. Dis., 183: 492 – 497.en_US
dc.identifier.citedreferenceXiao, L., Bern, C., Sulaiman, I.M. & Lal, A.A. 2004. Molecular epidemiology of human cryptosporidiosis. In: Thompson, R.C.A., ( ed. ), Cryptosporidium: From Molecules to Disease, Elsevier, p. 121 – 146.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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