Leukotrienes B 4 , C 4 and D 4 stimulate DNA synthesis in cultured human epidermal keratinocytes
dc.contributor.author | Kragballe, K. | en_US |
dc.contributor.author | Desjarlais, L. | en_US |
dc.contributor.author | Voorhees, John J. | en_US |
dc.date.accessioned | 2010-06-01T21:46:17Z | |
dc.date.available | 2010-06-01T21:46:17Z | |
dc.date.issued | 1985-07 | en_US |
dc.identifier.citation | KRAGBALLE, K.; DESJARLAIS, L.; VOORHEES, J.J. (1985). "Leukotrienes B 4 , C 4 and D 4 stimulate DNA synthesis in cultured human epidermal keratinocytes." British Journal of Dermatology 113(1): 43-52. <http://hdl.handle.net/2027.42/74813> | en_US |
dc.identifier.issn | 0007-0963 | en_US |
dc.identifier.issn | 1365-2133 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/74813 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2990516&dopt=citation | en_US |
dc.description.abstract | Leukotrienes in psoriatic skin lesions are potent mediators of inflammation. We have studied the capacity of leukotrienes to stimulate the DNA synthesis of cultured human epidermal keratinocytes. At concentrations ranging from 10 −12 to 10 −8 M, LTB 4 produced a 100% increase of DNA synthesis determined both as the incorporation of [ 3 H] thymidine and as the labelling index. In comparison, LTB 4 had no effect on the DNA synthesis of dermal fibroblast cultures. 5S, 12S-LTB 4 and 5S, 12S-all-trans-LTB 4 did not change the DNA synthesis of keratinocytes, but the effect of LTB 4 was abolished in the presence of 5S, 12S-all- trans HLTB 4 . Being less potent than LTB 4 the peptidoleukotrienes (LTC 4 , LTD 4 ) also stimulated keratinocyte DNA synthesis. The effect of the peptidoleukotrienes, but not of LTB 4 , was antagonized by EPL 55712. These results show that leukotrienes B 4 , C 4 and D 4 exert potent and stereospecific mitogenic effects on cultured human keratinocytes. The presence of these arachidonic acid metabolites in psoriatic skin lesions may be pertinent to both inflammation and aberrant epidermal growth in psoriasis. | en_US |
dc.format.extent | 3083600 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1985 British Association of Dermatologists | en_US |
dc.title | Leukotrienes B 4 , C 4 and D 4 stimulate DNA synthesis in cultured human epidermal keratinocytes | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dermatology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 2990516 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/74813/1/j.1365-2133.1985.tb02043.x.pdf | |
dc.identifier.doi | 10.1111/j.1365-2133.1985.tb02043.x | en_US |
dc.identifier.source | British Journal of Dermatology | en_US |
dc.identifier.citedreference | Allen, B.R. & Littlewood, S.M. ( 1982 ) Benoxaprofen: effect on cutaneous lesions of psoriasis. British Medical Journal, 285, 1241. | en_US |
dc.identifier.citedreference | Augstein, J., Farmer, J.B., Sheard, P. & Tattersall, M.L. ( 1973 ) Selective inhibitor of slow reacting substance of anaphylaxis, Nature New Biology, 254, 215 – 217. | en_US |
dc.identifier.citedreference | Bisgaard, H., Kristensen, J. & SÖndergaard, J. ( 1982 ) The effect of leukotriene C 4 and D 4 on cutaneous blood flow in humans. Prostaglandins, 23, 797. | en_US |
dc.identifier.citedreference | Borregaard, N. & Kragballe, K. ( 1980 ) The role of oxygen in antibody-dependent cytotoxicity mediated by monocytes and neutrophils. Journal of Clinical Investigation, 66, 676. | en_US |
dc.identifier.citedreference | Brain S.D., Camp, R.D.R., Leigh, I.M. & Ford-Hutchinson, A.W. ( 1982 ) The synthesis of leukotriene B 4 -like material by cultured human keratinocytes. Journal of Investigative Dermatology, 78, 328 ( abstract ). | en_US |
dc.identifier.citedreference | Brain, S.D., Camp, R.D.R., Charleson, S., Dowd, P.M., Ford-Hutchinson, A.W., Greaves, M.W. & Black, A.K. ( 1984a ) The release of LTC 4 -like material from the involved lesional skin in psoriasis. British Journal of Clinical Pharmacology, 17, 650P. | en_US |
dc.identifier.citedreference | Brain, S.D., Camp, R.D.R., Cunningham, F.M., Dowd, P.M., Greaves, M.W. & Black, A.K. ( 1984b ) Leukotriene B 4 -like material in scale of psoriatic skin lesion. British Journal of Pharmacology, 83, 313. | en_US |
dc.identifier.citedreference | Camp, R., Russell Jones, R., Brain, S., Woolard, P. & Greaves, M. ( 1984 ) Production of intraepidermal microabscesses by topical application of leukotriene B 4. Journal of Investigative Dermatology, 82, 202. | en_US |
dc.identifier.citedreference | DahlÉn, S-E., Hedqvist, P., HammarstrÖm, S. & Sammuelsson, B. ( 1980 ) Leukotrienes are potent constrictors of human bronchi. Nature, 228, 484. | en_US |
dc.identifier.citedreference | Feinmark, S.J., Lindgren, J.A., Claesson, H., Malmsten, C. & Samuelsson, B. ( 1981 ) Stimulation of human leukocyte degranulation by leukotriene B 4 and its Ø-oxidized metabolites. FEBS Letters, 136, 141. | en_US |
dc.identifier.citedreference | Ford-Hutchinson, A.W., Bray, M.A., Boig, M.V., Shipley, M.E. & Smith, M.J.H. ( 1980 ) Leukotriene B, a potent chemokinetic and aggregating substance from polymorphonuclear leukocytes. Nature, 286, 264. | en_US |
dc.identifier.citedreference | Goldman, D.W. & Goetzl, E.J. ( 1982 ) Specific binding of leukotriene B 4 to receptors on human polymorphonuclear leukocytes. Journal of Immunology, 129, 1600. | en_US |
dc.identifier.citedreference | HammarstrÖm, S., Hamberg, M., Samuelsson, B., Duell, E.A., Stawiski, M. & Voorhees, J.J. ( 1975 ) Increased concentrations of nonesterified arachidonic acid, 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid, prostaglandin E 2, prostaglandin F 2 in epidermis of psoriasis. Proceedings of the National Academy of Sciences, U.S.A., 72, 5130. | en_US |
dc.identifier.citedreference | Hedqvist, P., DahlÉn, S-E., Gustavsson, L., HammarstrÖm, S. & Samuelsson, B. ( 1980 ) Biological profile of leukotrienes C 4 and D 4. Acta Physiologica Scandinavica, 110, 331. | en_US |
dc.identifier.citedreference | Johnson, H.M. & Torres, B.A. ( 1984 ) Leukotriences: positive signals for regulation of Γ-interferon production. Journal of Immunology, 132, 413. | en_US |
dc.identifier.citedreference | Kragballe, K. & Herlin, T. ( 1983 ) Benoxaprofen improves psoriasis. A double-blind study. Archives of Dermatology, 119, 548. | en_US |
dc.identifier.citedreference | Lewis, R.A., Frazen, J.M., Austen, K.E., Toda, M., Brion, F., Margat, A. & Corey, E.J. ( 1981 ) Contractile properties of leukotrienes C and D: role of the polar substituents. Proceedings of the National Academy of Sciences, U.S.A. 78, 4579. | en_US |
dc.identifier.citedreference | Liu, S.C. & Karasek, M. ( 1978 ) Isolation and growth of adult human epidermal keratinocytes. Journal of Investigative Dermatology, 71, 157. | en_US |
dc.identifier.citedreference | Malmsten, C.L., Palmblad, J., UdÉn, A.-M., Radmark, O., Engstedt, L. & Samuelsson, B. ( 1980 ) Leukotriene B 4: A highly potent and stereospecific factor stimulating migration of polymorphonuclear leukocytes. Acta Physiologica Scandinavica, 110, 449. | en_US |
dc.identifier.citedreference | Marcelo, C.L., Kim, Y.G., Kaine, J.L. & Voorhees, J.J. ( 1978 ) Stratification, specialization, and proliferation of primary keratinocyte cultures. Journal of Cell Biology, 79, 356. | en_US |
dc.identifier.citedreference | Marcelo, C.L. & Tomich, J. ( 1983 ) Cyclic AMP, glucocorticoid, and retinoid modulation of in vitro keratinocyte growth. Journal of Investigative Dermatology, 81, 64s. | en_US |
dc.identifier.citedreference | Mensing, H. & Czarnetzki, B.M. ( 1984 ) Leukotriene B 4 induces in vitro fibroblast chemotaxis. Journal of Investigative Dermatology, 82, 9. | en_US |
dc.identifier.citedreference | Nagy, L., Lee, T.H., Goetzl, E.J., Pickett, W.C. & Kay, B. ( 1982 ) Complement receptor enhancement and chemotaxis of human neutrophils and eosinophils by leukotrienes and other lipoxygenase products. Clinical and Experimental Immunology, 47, 541. | en_US |
dc.identifier.citedreference | Örning, L. & HammarstrÖm, S. ( 1980 ) Inhibition of leukotriene C and D biosynthesis. Journal of Biological Chemistry, 255, 8023. | en_US |
dc.identifier.citedreference | Samuelsson, B. ( 1983 ) Leukotrienes: mediators of immediate hypersensitivity reactions and inflammation. Science, 220, 568. | en_US |
dc.identifier.citedreference | Serhan, C.M., Radin, A., Smolen, J.E., Korchak, H., Samuelsson, B. & Weissman, G. ( 1982 ) Leukotriene B 4 is a complete secretagogue in human neutrophils: A kinetic analysis. Biochemical and Biophysical Research Communications, 107, 1006 – 1012. | en_US |
dc.identifier.citedreference | Smith, M.J.H. & Walker, J.R. ( 1980 ) The effect of some antirheumatic drugs on an in vitro model of human polymorphonuclear leucocyte chemokinesis. British Journal of Pharmacology, 69, 473. | en_US |
dc.identifier.citedreference | Soter, N.A., Lewis, R.A., Corey, E.J. & Austen, K.E. ( 1983 ) Local effects of synthetic leukotrienes (LTC 4, LTD 4 and LTB 4 ) in human skin. Journal of Investigative Dermatology, 80, 115. | en_US |
dc.identifier.citedreference | Wicha, M.S., Liotta, L.A. & Kidwell, W.R. ( 1979 ) Effects of free fatty acids on the growth of normal and neoplastic rat mammary epithelial cells. Cancer Research, 39, 426. | en_US |
dc.identifier.citedreference | Ziboh, V.A., Casebolt, T., Marcelo, C.L. & Voorhees, J.J. ( 1983 ) Enhancement of 5-lipoxygenase activity on soluble preparations of human psoriatic plaque preparation. Journal of Investigative Dermatology, 80, 359. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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