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Type 1 fimbriae mutants of Escherichia coli K12: characterization of recognized afimbriate strains and construction of new fim deletion mutants

dc.contributor.authorBlomfjeld, I. C.en_US
dc.contributor.authorMcClain, M. S.en_US
dc.contributor.authorEisenstein, Barry I.en_US
dc.date.accessioned2010-06-01T21:53:34Z
dc.date.available2010-06-01T21:53:34Z
dc.date.issued1991-06en_US
dc.identifier.citationBlomfjeld, I. C.; McClain, M. S.; Eisenstein, B. I. (1991). "Type 1 fimbriae mutants of Escherichia coli K12: characterization of recognized afimbriate strains and construction of new fim deletion mutants." Molecular Microbiology 5(6): 1439-1445. <http://hdl.handle.net/2027.42/74928>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/74928
dc.description.abstractWe have used Southern hybridization analysis to characterize the extent of fim homology in recognized type 1 fimbriae mutants of Escherichia coli K12, including strains HB101, P678-54, and VL584. We have found extensive homology in strain HB101, and confirm that P678-54 lacks the majority of fim DNA. Strain VL584 contains a deletion of the entire fim region. We have used a new allelic exchange procedure to generate novel fim deletion derivatives of strains MG1655, MM294, and YMC9. To increase the utility of the new deletion strains we also isolated recA derivatives of each mutant. These strains facilitate the isolation, characterization, and manipulation of cloned fimbriae genes from diverse sources.en_US
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dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights1991 Blackwell Publishingen_US
dc.titleType 1 fimbriae mutants of Escherichia coli K12: characterization of recognized afimbriate strains and construction of new fim deletion mutantsen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, 6643 Medical Sciences Building II, Ann Arbor, Michigan 48109, USA.en_US
dc.identifier.pmid1686292en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/74928/1/j.1365-2958.1991.tb00790.x.pdf
dc.identifier.doi10.1111/j.1365-2958.1991.tb00790.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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