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Flow cytometric and morphological characterization of platelet-rich plasma gel

dc.contributor.authorFernández-Barbero, Juan Emilioen_US
dc.contributor.authorGalindo-Moreno, Pabloen_US
dc.contributor.authorÁvila-Ortiz, Gustavoen_US
dc.contributor.authorCaba, Octavioen_US
dc.contributor.authorSánchez-Fernández, E.en_US
dc.contributor.authorWang, Hom-Layen_US
dc.date.accessioned2010-06-01T22:05:17Z
dc.date.available2010-06-01T22:05:17Z
dc.date.issued2006-12en_US
dc.identifier.citationFernÁndez-Barbero, Juan Emilio; Galindo-Moreno, Pablo; Ávila-Ortiz, Gustavo; Caba, Octavio; SÁnchez-FernÁndez, E.; Wang, Hom-Lay (2006). "Flow cytometric and morphological characterization of platelet-rich plasma gel." Clinical Oral Implants Research 17(6): 687-693. <http://hdl.handle.net/2027.42/75112>en_US
dc.identifier.issn0905-7161en_US
dc.identifier.issn1600-0501en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/75112
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17092228&dopt=citationen_US
dc.description.abstractBackground of problems : Platelet-rich plasma (PRP) gel is derived from an autogenous preparation of concentrated platelets and is widely used in implant dentistry as a vector for cell growth factors. However, limited data are available on its structure and composition. The present study was aimed at providing a flow cytometric and ultrastructural characterization of PRP gel. Materials and methods : Twenty PRP gel samples were obtained from healthy volunteers. These PRP gel specimens were prepared for transmission (TEM) and scanning electron microscopy (SEM) examination of their morphological ultrastructure. Flow cytometry with CD41-PE monoclonal antibody was used to detect platelet cells, as this antibody recognizes human-platelet-specific antigen CD41. Results : Both SEM and TEM showed that PRP gel contains two components: a fibrillar material with striated band similar to fibrin filaments, and a cellular component that contains human platelet cells. Both techniques indicated that no morphological elements were bound between the cellular component and the fibrillar material. The cells were confirmed as platelet cells by flow cytometric study after incubation with specific monoclonal antibody CD41-PE. Conclusion : PRP gel contains a fibrillar and a cellular (largely human platelet cell) component. This unique structure may be capable of acting as a vehicle for carrying of cells that are essential for soft/hard tissue regeneration. To cite this article: FernÁndez-Barbero JE, Galindo-Moreno P, Ávila-Ortiz G, Caba O, SÁnchez-FernÁndez E, Wang H-L. Flow cytometric and morphological characterization of platelet-rich plasma gel. Clin. Oral Impl. Res . 17, 2006; 687–693 doi: 10.1111/j.1600-0501.2006.01179.xen_US
dc.format.extent1101449 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rightsCopyright © Blackwell Munksgaard 2006en_US
dc.subject.otherGrowth Factorsen_US
dc.subject.otherPlatelet-rich Plasma (PRP)en_US
dc.subject.otherTissue Engineeringen_US
dc.subject.otherWound Healingen_US
dc.titleFlow cytometric and morphological characterization of platelet-rich plasma gelen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelDentistryen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Periodontics/Prevention/Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, MI, USAen_US
dc.contributor.affiliationotherHuman Anatomy and Embryology Department, School of Medicine, University of Granada, Granada, Spainen_US
dc.contributor.affiliationotherOral Surgery Department, School of Dentistry, University of Granada, Granada, Spainen_US
dc.identifier.pmid17092228en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/75112/1/j.1600-0501.2006.01179.x.pdf
dc.identifier.doi10.1111/j.1600-0501.2006.01179.xen_US
dc.identifier.sourceClinical Oral Implants Researchen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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