Selection of nitrogen-fixing deficient Burkholderia vietnamiensis strains by cystic fibrosis patients: involvement of nif gene deletions and auxotrophic mutations
dc.contributor.author | Menard, Aymeric | en_US |
dc.contributor.author | Monnez, Claire | en_US |
dc.contributor.author | Estrada de los Santos, Paulina | en_US |
dc.contributor.author | Segonds, Christine | en_US |
dc.contributor.author | Caballero-Mellado, Jesus | en_US |
dc.contributor.author | LiPuma, John J. | en_US |
dc.contributor.author | Chabanon, Gerard | en_US |
dc.contributor.author | Cournoyer, Benoit | en_US |
dc.date.accessioned | 2010-06-01T22:08:45Z | |
dc.date.available | 2010-06-01T22:08:45Z | |
dc.date.issued | 2007-05 | en_US |
dc.identifier.citation | Menard, Aymeric; Monnez, Claire; Estrada de los Santos, Paulina; Segonds, Christine; Caballero-Mellado, Jesus; LiPuma, John J.; Chabanon, Gerard; Cournoyer, Benoit (2007). "Selection of nitrogen-fixing deficient Burkholderia vietnamiensis strains by cystic fibrosis patients: involvement of nif gene deletions and auxotrophic mutations." Environmental Microbiology 9(5): 1176-1185. <http://hdl.handle.net/2027.42/75167> | en_US |
dc.identifier.issn | 1462-2912 | en_US |
dc.identifier.issn | 1462-2920 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/75167 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17472633&dopt=citation | en_US |
dc.description.abstract | Burkholderia vietnamiensis is the third most prevalent species of the Burkholderia cepacia complex (Bcc) found in cystic fibrosis (CF) patients. Its ability at fixing nitrogen makes it one of the main Bcc species showing strong filiations with environmental reservoirs. In this study, 83% (29 over 35) of the B. vietnamiensis CF isolates and 100% of the environmental ones (over 29) were found expressing the dinitrogenase complex (encoded by the nif cluster) which is essential in N 2 fixation. Among the deficient strains, two were found growing with ammonium chloride suggesting that they were defective in N 2 fixation, and four with amino acids supplements suggesting that they were harbouring auxotrophic mutations. To get insights about the genetic events that led to the emergence of the N 2 -fixing defective strains, a genetic analysis of B. vietnamiensis nitrogen-fixing property was undertaken. A 40-kb-long nif cluster and nif regulatory genes were identified within the B. vietnamiensis strain G4 genome sequence, and analysed. Transposon mutagenesis and nifH genetic marker exchanges showed the nif cluster and several other genes like gltB (encoding a subunit of the glutamate synthase) to play a key role in B. vietnamiensis ability at growing in nitrogen-free media. nif cluster DNA probings of restricted genomic DNA blots showed a full deletion of the nif cluster for one of the N 2 -fixing defective strain while the other one showed a genetic organization similar to the one of the G4 strain. For 17% of B. vietnamiensis clinical strains, CF lungs appeared to have favoured the selection of mutations or deletions leading to N 2 -fixing deficiencies. | en_US |
dc.format.extent | 279045 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | © 2007 The Authors; Journal compilation © 2007 Society for Applied Microbiology and Blackwell Publishing Ltd | en_US |
dc.title | Selection of nitrogen-fixing deficient Burkholderia vietnamiensis strains by cystic fibrosis patients: involvement of nif gene deletions and auxotrophic mutations | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109, USA. | en_US |
dc.contributor.affiliationother | Research group on «Bacterial Opportunistic Pathogens and Environment», UniversitÉ de Lyon, Lyon, F-69003, France; UniversitÉ Lyon 1, Villeurbanne, F-69622, France; CNRS, UMR5557, Ecologie Microbienne, Villeurbanne, F-69622; Ecole Nationale VÉtÉrinaire de Lyon, Marcy L'Étoile, F-69280, France. | en_US |
dc.contributor.affiliationother | Observatoire cepacia, Laboratoire de Bacteriologie-Hygiene, Institut Federatif de Biologie, HÔpital Purpan, TSA 40031, 31059 Toulouse Cedex 9, France. | en_US |
dc.contributor.affiliationother | Centro de Ciencias GenÓmicas, Universidad Nacional AutÓnoma de MÉxico, Ap. Postal 565-A, Cuernavaca, Morelos, Mexico. | en_US |
dc.identifier.pmid | 17472633 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/75167/1/j.1462-2920.2007.01240.x.pdf | |
dc.identifier.doi | 10.1111/j.1462-2920.2007.01240.x | en_US |
dc.identifier.source | Environmental Microbiology | en_US |
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