hTERT expression in melanocytic lesions: an immunohistochemical study on paraffin-embedded tissue
dc.contributor.author | Fullen, Douglas R. | en_US |
dc.contributor.author | Zhu, Weijian | en_US |
dc.contributor.author | Thomas, Dafydd G. | en_US |
dc.contributor.author | Su, Lyndon D. | en_US |
dc.date.accessioned | 2010-06-01T22:32:11Z | |
dc.date.available | 2010-06-01T22:32:11Z | |
dc.date.issued | 2005-11 | en_US |
dc.identifier.citation | Fullen, Douglas R.; Zhu, Weijian; Thomas, Dafydd; Su, Lyndon D. (2005). "hTERT expression in melanocytic lesions: an immunohistochemical study on paraffin-embedded tissue." Journal of Cutaneous Pathology 32(10): 680-684. <http://hdl.handle.net/2027.42/75519> | en_US |
dc.identifier.issn | 0303-6987 | en_US |
dc.identifier.issn | 1600-0560 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/75519 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16293180&dopt=citation | en_US |
dc.description.abstract | Telomerase plays a role in the immortalization of cells and carcinogenesis. Previous studies have yielded conflicting results on whether human telomerase RNA (hTER) expression differs in nevi, atypical nevi and melanomas using polymerase chain reaction-based telomeric repeat amplification protocol or in situ hybridization assays. The aim of this study was to evaluate human telomerase reverse transcriptase (hTERT) staining in melanocytic lesions on paraffin-embedded tissues. Methods: Paraffin-embedded sections from 12 acquired nevi, seven dysplastic nevi, 11 Spitz nevi, eight primary invasive melanomas, and three metastatic melanomas were studied for staining intensity (0–3+) and percentage of labeled cells with anti-hTERT. Results: hTERT staining was observed in most cells (>75%), in all but three lesions, and was of greater intensity in the nucleus, especially the nucleolus, compared with the cytoplasm. Spitz nevi tended to have weaker hTERT staining (mean = 1.7) compared with acquired nevi (mean = 2.2), dysplastic nevi (mean = 2.4), primary melanomas (mean = 2.4), or metastatic melanomas (mean = 3). Conclusions: Although telomerase activity was weaker in Spitz nevi, there was overlap with other nevi and primary invasive melanomas in our small series. Thus, hTERT expression does not appear to be a reliable adjunct to the histological diagnosis of primary melanocytic lesions. Fullen DR, Zhu W, Thomas D, Su LD. hTERT expression in melanocytic lesions: an immunohistochemical study on paraffin-embedded tissue. | en_US |
dc.format.extent | 335337 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Munksgaard International Publishers | en_US |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | Blackwell Munksgaard, 2005 | en_US |
dc.title | hTERT expression in melanocytic lesions: an immunohistochemical study on paraffin-embedded tissue | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Dermatology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Pathology, | en_US |
dc.contributor.affiliationother | Department of Dermatology, and | en_US |
dc.identifier.pmid | 16293180 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/75519/1/j.0303-6987.2005.00403.x.pdf | |
dc.identifier.doi | 10.1111/j.0303-6987.2005.00403.x | en_US |
dc.identifier.source | Journal of Cutaneous Pathology | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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