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Flow cytometry for microbial sensing in environmental sustainability applications: current status and future prospects

dc.contributor.authorGruden, Cyndee L.en_US
dc.contributor.authorSkerlos, Steven J.en_US
dc.contributor.authorAdriaens, Peteren_US
dc.date.accessioned2010-06-01T22:38:06Z
dc.date.available2010-06-01T22:38:06Z
dc.date.issued2004-07en_US
dc.identifier.citationGruden, Cyndee; Skerlos, Steven; Adriaens, Peter (2004). "Flow cytometry for microbial sensing in environmental sustainability applications: current status and future prospects." FEMS Microbiology Ecology 49(1): 37-49. <http://hdl.handle.net/2027.42/75610>en_US
dc.identifier.issn0168-6496en_US
dc.identifier.issn1574-6941en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/75610
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19712382&dopt=citationen_US
dc.description.abstractPractical and accurate microbial assessment of environmental systems is predicated on the detection and quantification of various microbial parameters in complex matrices. Traditional growth-based assays, considered to be both slow and biased, are increasingly being replaced by optical detection methods such as flow cytometry. Flow cytometry (FCM) offers high-speed multi-parametric data acquisition, compatibility with current molecular-based microbial detection technologies, and is a proven technology platform. The unique technical properties of flow cytometry have allowed the discrimination of bacteria based on nucleic acid staining, microbial identification based on genomic and immunologic characteristics, and determination of cell viability. For this technology to achieve the ultimate goal of monitoring the microbial ecology of distributed systems, it will be necessary to develop a fully functional, low cost, and networkable microsystem platform capable of rapid detection of multiple species of microorganisms simultaneously under realistic environmental conditions. One such microsystem, miniaturized and integrated in accordance with recent advances in micro-electro-mechanical systems technology, is named the Micro Integrated Flow Cytometer. This manuscript is a minireview of the current status and future prospects for environmental application of flow cytometry in general, and micro-flow cytometry in particular.en_US
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dc.publisherBlackwell Publishing Ltden_US
dc.rights2004 Federation of European Microbiological Societiesen_US
dc.subject.otherFlow Cytometryen_US
dc.subject.otherMicrobial Sensingen_US
dc.subject.otherEnvironmental Applicationen_US
dc.subject.otherMonitoring Networken_US
dc.titleFlow cytometry for microbial sensing in environmental sustainability applications: current status and future prospectsen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Civil and Environmental Engineering, University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationotherDepartment of Civil Engineering, University of Toledo, Toledo, OH 43606, USAen_US
dc.identifier.pmid19712382en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/75610/1/j.femsec.2004.01.014.pdf
dc.identifier.doi10.1016/j.femsec.2004.01.014en_US
dc.identifier.sourceFEMS Microbiology Ecologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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