RNG1 is a late marker of the apical polar ring in Toxoplasma gondii
dc.contributor.author | Tran, Johnson Q. | en_US |
dc.contributor.author | de Leon, Jessica C. | en_US |
dc.contributor.author | Li, Catherine | en_US |
dc.contributor.author | Huynh, My-Hang | en_US |
dc.contributor.author | Beatty, Wandy | en_US |
dc.contributor.author | Morrissette, Naomi S. | en_US |
dc.date.accessioned | 2010-09-02T15:21:24Z | |
dc.date.available | 2011-03-01T16:26:45Z | en_US |
dc.date.issued | 2010-09 | en_US |
dc.identifier.citation | Tran, Johnson Q.; de Leon, Jessica C.; Li, Catherine; Huynh, My-Hang; Beatty, Wandy; Morrissette, Naomi S. (2010). "RNG1 is a late marker of the apical polar ring in Toxoplasma gondii." Cytoskeleton 67(9): 586-598. <http://hdl.handle.net/2027.42/77953> | en_US |
dc.identifier.issn | 1949-3584 | en_US |
dc.identifier.issn | 1949-3592 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/77953 | |
dc.description.abstract | The asexually proliferating stages of apicomplexan parasites cause acute symptoms of diseases such as malaria, cryptosporidiosis and toxoplasmosis. These stages are characterized by the presence of two independent microtubule organizing centers (MTOCs). Centrioles are found at the poles of the intranuclear spindle. The apical polar ring (APR), a MTOC unique to apicomplexans, organizes subpellicular microtubules which impose cell shape and apical polarity on these protozoa. Here we describe the characteristics of a novel protein that localizes to the APR of Toxoplasma gondii which we have named ring-1 (RNG1). There are related RNG1 proteins in Neospora caninum and Sarcocystis neurona but no obvious homologs in Plasmodium spp., Cryptosporidium spp. or Babesia spp. RNG1 is a small, low-complexity, detergent-insoluble protein that assembles at the APR very late in the process of daughter parasite replication. We were unable to knock-out the RNG1 gene, suggesting that its gene product is essential. Tagged RNG1 lines have also allowed us to visualize the APR during growth of Toxoplasma in the microtubule-disrupting drug oryzalin. Oryzalin inhibits nuclear division and cytokinesis although Toxoplasma growth continues, and similar to earlier observations of unchecked centriole duplication in oryzalin-treated parasites, the APR continues to duplicate during aberrant parasite growth. © 2010 Wiley-Liss, Inc. | en_US |
dc.format.extent | 956966 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | RNG1 is a late marker of the apical polar ring in Toxoplasma gondii | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan School of Medicine, Ann Arbor Michigan 48109 | en_US |
dc.contributor.affiliationother | Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697 | en_US |
dc.contributor.affiliationother | Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697 | en_US |
dc.contributor.affiliationother | Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697 | en_US |
dc.contributor.affiliationother | Department of Molecular Microbiology, Washington University School of Medicine, St. Louis Missouri 63110 | en_US |
dc.contributor.affiliationother | Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697 ; Department of Molecular Biology and Biochemistry, University of California, Irvine CA 92697-3900 | en_US |
dc.identifier.pmid | 20658557 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/77953/1/20469_ftp.pdf | |
dc.identifier.doi | 10.1002/cm.20469 | en_US |
dc.identifier.source | Cytoskeleton | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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