Expression patterns of corticotropin-releasing factor, arginine vasopressin, histidine decarboxylase, melanin-concentrating hormone, and orexin genes in the human hypothalamus
dc.contributor.author | Krolewski, David M. | en_US |
dc.contributor.author | Medina, Adriana | en_US |
dc.contributor.author | Kerman, Ilan A. | en_US |
dc.contributor.author | Bernard, Rene | en_US |
dc.contributor.author | Burke, Sharon | en_US |
dc.contributor.author | Thompson, Robert C. | en_US |
dc.contributor.author | Bunney, William E. | en_US |
dc.contributor.author | Schatzberg, Alan F. | en_US |
dc.contributor.author | Myers, Richard M. | en_US |
dc.contributor.author | Akil, Huda | en_US |
dc.contributor.author | Jones, Edward G. | en_US |
dc.contributor.author | Watson, Stanley J. | en_US |
dc.date.accessioned | 2010-10-06T14:57:20Z | |
dc.date.available | 2011-03-01T16:26:44Z | en_US |
dc.date.issued | 2010-11-15 | en_US |
dc.identifier.citation | Krolewski, David M.; Medina, Adriana; Kerman, Ilan A.; Bernard, Rene; Burke, Sharon; Thompson, Robert C.; Bunney, William E.; Schatzberg, Alan F.; Myers, Richard M.; Akil, Huda; Jones, Edward G.; Watson, Stanley J. (2010). "Expression patterns of corticotropin-releasing factor, arginine vasopressin, histidine decarboxylase, melanin-concentrating hormone, and orexin genes in the human hypothalamus." The Journal of Comparative Neurology 518(22): 4591-4611. <http://hdl.handle.net/2027.42/78077> | en_US |
dc.identifier.issn | 0021-9967 | en_US |
dc.identifier.issn | 1096-9861 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/78077 | |
dc.description.abstract | The hypothalamus regulates numerous autonomic responses and behaviors. The neuroactive substances corticotropin-releasing factor (CRF), arginine-vasopressin (AVP), histidine decarboxylase (HDC), melanin-concentrating hormone (MCH), and orexin/hypocretins (ORX) produced in the hypothalamus mediate a subset of these processes. Although the expression patterns of these genes have been well studied in rodents, less is known about them in humans. We combined classical histological techniques with in situ hybridization histochemistry to produce both 2D and 3D images and to visually align and quantify expression of the genes for these substances in nuclei of the human hypothalamus. The hypothalamus was arbitrarily divided into rostral, intermediate, and caudal regions. The rostral region, containing the paraventricular nucleus (PVN), was defined by discrete localization of CRF- and AVP-expressing neurons, whereas distinct relationships between HDC, MCH, and ORX mRNA-expressing neurons delineated specific levels within the intermediate and caudal regions. Quantitative mRNA signal intensity measurements revealed no significant differences in overall CRF or AVP expression at any rostrocaudal level of the PVN. HDC mRNA expression was highest at the level of the premammillary area, which included the dorsomedial and tuberomammillary nuclei as well as the dorsolateral hypothalamic area. In addition, the overall intensity of hybridization signal exhibited by both MCH and ORX mRNA-expressing neurons peaked in distinct intermediate and caudal hypothalamic regions. These results suggest that human hypothalamic neurons involved in the regulation of the HPA axis display distinct neurochemical patterns that may encompass multiple local nuclei. J. Comp. Neurol. 518:4591–4611, 2010. © 2010 Wiley-Liss, Inc. | en_US |
dc.format.extent | 1685527 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | Expression patterns of corticotropin-releasing factor, arginine vasopressin, histidine decarboxylase, melanin-concentrating hormone, and orexin genes in the human hypothalamus | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan 48109 ; Department of Psychiatry, University of California, Irvine, California 92697 | en_US |
dc.contributor.affiliationum | Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationother | Department of Psychiatry, University of California, Irvine, California 92697 | en_US |
dc.contributor.affiliationother | Psychiatry Department, Stanford University School of Medicine, Stanford, California 94305 | en_US |
dc.contributor.affiliationother | HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806 | en_US |
dc.contributor.affiliationother | Center for Neuroscience, University of California, Davis, California 956108 ; Center for Neuroscience, 1544 Newton Court, Davis, CA 95618 | en_US |
dc.identifier.pmid | 20886624 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/78077/1/22480_ftp.pdf | |
dc.identifier.doi | 10.1002/cne.22480 | en_US |
dc.identifier.source | The Journal of Comparative Neurology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.