Now showing items 11-20 of 59
Proteomic analysis of cold adaptation in a Siberian permafrost bacterium – Exiguobacterium sibiricum 255–15 by two-dimensional liquid separation coupled with mass spectrometry
(WILEY-VCH Verlag, 2006-10)
Bacterial cold adaptation in Exiguobacterium sibiricum 255–15 was studied on a proteomic scale using a 2-D liquid phase separation coupled with MS technology. Whole-cell lysates of E. sibiricum 255–15 grown at 4°C and ...
Structure and Activity of the Photosystem II Manganese-Stabilizing Protein: Role of the Conserved Disulfide Bond
(Kluwer Academic Publishers; Springer, 2005-09)
The 33-kDa manganese-stabilizing protein (MSP) of Photosystem II (PS II) maintains the functional stability of the Mn cluster in the enzyme’s active site. This protein has been shown to possess characteristics similar to ...
Fluorescence-based biosensing of zinc using carbonic anhydrase
(Kluwer Academic Publishers; Springer Science+Business Media, 2001-09)
Measurement of free zinc levels and imaging of zinc fluxes remains technically difficult due to low levels and the presence of interfering cations such as Mg and Ca. We have developed a series of fluorescent zinc indicators ...
Cation-induced, inhibitor-resistant photosystem II reactions in cyanobacterial membranes
(Elsevier, 1981-06-16)
Ferricyanide-supported oxygen evolution in sonic vesicles from the cyanobacterium is only partially sensitive to inhibition by 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), and addition of cations to inhibited ...
Characterization of a photosystem II reaction center complex isolated by exposure of PSII membranes to a non-ionic detergent and high concentrations of NaCl
(Martinus Nijhoff, The Hague/Kluwer Academic Publishers; Martinus Nijhoff Publishers ; Springer Science+Business Media, 1986-01)
A highly resolved PSII reaction center complex has been prepared by exposure of PSII membranes to the detergent octylglucopyranoside at elevated ionic strengths; oxygen evolution activity is about 1,000 μmoles O 2 /hr/mg ...
The production of superoxide anion radicals in the reaction of reduced flavins and flavoproteins with molecular oxygen
(Elsevier, 1969-09-10)
The generation of O2-- in the reaction of reduced flavins and several flavoproteins has been shown. This has been demonstrated by the ability to catalyse the aerobic reduction of cytochrome and the inhibition of this ...
NMR paramagnetic relaxation enhancements due to manganese in the S 0 and S 2 states of Photosystem II-enriched membrane fragments and in the detergent-solubilized Photosystem II complex
(Kluwer Academic Publishers; Springer Science+Business Media, 1993-01)
The NMR paramagnetic relaxation enhancement (NMR-PRE) produced in the solvent proton resonance by manganese in the S 0 and S 2 states of the oxygen evolving center (OEC) has been recorded for three Photosystem II (PS ...
Cobalamin-dependent methionine synthase: the structure of a methylcobalamin-binding fragment and implications for other B12-dependent enzymes
(Elsevier, 1994)
Cobalamin-dependent methionine synthase is a large enzyme composed of structurally and functionally distinct regions. Recent studies have begun to define the roles of several regions of the protein. In particular, the ...
Pre-resonance Raman spectra of adriamycin
(Elsevier, 1976-08-23)
Pre-resonance Raman spectra have been obtained for aqueous solutions of adriamycin. All bands are attributable to the anthracycline chromophore and are vibrations coupled to the first (20,800 cm-1) electronic transition ...
Heterobifunctional cross-linking of a monoclonal antibody with 2-methyl-N1-benzenesulfonyl-N4-bromoacetylquinonediimide
(Elsevier, 1989-02-15)
The Cyssor reagent, 2-methyl-N1-benzenesulfonyl-N4-bromoacetylquinonediimide, which will cleave a protein chain at Cys under acidic conditions, cross-linked unreduced and partially reduced antibody at pH 8.0. No cleavage ...