Now showing items 21-30 of 59
The identification of phosphoglycerate kinase-1 and histone H4 autoantibodies in pancreatic cancer patient serum using a natural protein microarray
(WILEY-VCH Verlag, 2009-06)
Protein microarrays have been used to explore whether a humoral response to pancreatic cancer-specific tumor antigens has utility as a biomarker of pancreatic cancer. To determine if such arrays can be used to identify ...
A CE assay for the detection of agonist-stimulated adenylyl cyclase activity
(WILEY-VCH Verlag, 2007-06)
A CE assay was developed for the detection of adenylyl cyclase (AC) activity stimulated at the AC and G protein-coupled receptor (GPCR) level. In the assay, cell membranes overexpressing GPCR and/or AC were incubated with ...
Proteomic profiling identifies breast tumor metastasis-associated factors in an isogenic model
(WILEY-VCH Verlag, 2007-01)
A combination of LC and MS was applied to an isogenic breast tumor metastasis model to identify proteins associated with a cellular phenotype. Chromatofocusing followed by nonporous-RP-HPLC/ESI-TOF MS was applied to cell ...
Stereochemical course of the biosynthesis of 1-aminocyclopropane-1-carboxylic acid. I. Role of the asymmetric sulfonium pole and the [alpha]-amino acid center
(Elsevier, 1984-05-31)
The substrate stereospecificity of 1-aminocyclopropane-1-carboxylic acid synthase, a pyridoxal phosphate-containing enzyme, from the pericarp tissue of (tomatoes) was studied using the various stereoisomers of -adenosylmethionine ...
Reconstitution of the spinach oxygen-evolving complex with recombinant Arabidopsis manganese-stabilizing protein
(Kluwer Academic Publishers; Springer Science+Business Media, 1994-10)
The psbO gene of cyanobacteria, green algae and higher plants encodes the precursor of the 33 kDa manganese-stabilizing protein (MSP), a water-soluble subunit of photosystem II (PSII). Using a pET-T7 cloning/expression ...
Measurement of dissociation rate of biomolecular complexes using CE
(WILEY-VCH Verlag, 2009-02)
Fluorescence anisotropy (FA), non-equilibrium CE of equilibrium mixtures (NECEEM) and high-speed CE were evaluated for measuring dissociation kinetics of peptide–protein binding systems. Fyn-SH3-SH2, a protein construct ...
A further investigation of the stannic chloride-catalyzed condensation reaction of 1-hexene and 1,2,3,5-tetra-O-acyl-[beta]--ribofuranoses
(Elsevier, 1983-04-16)
The reaction of 1-hexene with either 1-O-acetyl-2,3,5-tri-O-benzoyl-[beta]--ribofuranose (5b) or 1,2,3,5-tetra-O-acetyl-[beta]--ribofuranose (5a) in the presence of stannic chloride leads to the formation of a complex ...
Crystallization and preliminary X-ray diffraction studies of the cobalamin-binding domain of methionine synthase from Escherichia coli
(Elsevier, 1992-05-20)
Crystals of a cobalamin-binding domain (Mr = 28,000) have been grown in polyethylene glycol 6000 at pH 7.5, starting from solutions of intact (Mr = 133,000) cobalamin-dependent methionine synthase. The crystals are ...
Structure and function in the isolated reaction center complex of Photosystem II: energy and charge transfer dynamics and mechanism
(Kluwer Academic Publishers; Springer Science+Business Media, 2002-05)
The dynamics of energy and charge transfer in the Photosystem II reaction center complex is an area of great interest today. These processes occur on a time scale ranging from femtoseconds to tens of picoseconds or longer. ...
Strain-specific modification of lethality in fucose-deficient mice
(Springer-Verlag; Springer-Verlag New York Inc., 2003-02)
Abstract