Now showing items 21-30 of 49
Properties of the cyanobacterial coupling factor ATPase from Spirulina platensis : II. Activity of the purified and membrane-bound enzymes
(Elsevier, 1986-02-15)
Cyanobacterial (Spirulina platensis) photosynthetic membranes and isolated F1 ATPase were characterized with respect to ATP activity. The following results indicate that the regulation of expression of ATPase activity in ...
Heparin inhibition of ferredoxin-NADP reductase in chloroplast thylakoid membranes
(Elsevier, 1985-02-01)
Heparin, an anionic polysaccharide, inhibited the ferredoxin-catalyzed reduction of NADP in spinach chloroplast thylakoid membranes. Under the same conditions of assay, heparin did not interfere markedly with photoreduction ...
The interaction of nitrous oxide with cobalamin-dependent methionine synthase
(Elsevier, 1991-08)
Application of the gel shift assay to study the affinity and specificity of anti-DNA autoantibodies
(Elsevier, 1994-12-28)
We have demonstrated that the gel shift assay, a powerful method to study protein [middle dot] DNA interactions under equilibrium conditions, is both an accurate and precise method to measure the affinity of anti-DNA [middle ...
Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry
(Elsevier, 1989-04)
The effect of trimethylsilyl (TMS) derivatization on detection limits of mononucleotides in fast atom bombardment-mass spectrometry (FAB-MS) was examined. FAB-MS methods were developed to optimize sensitivity using adenosine ...
Fragmentative and stereochemical isomerization probes for homolytic carbon to phosphorus bond scission catalyzed by bacterial carbon-phosphorus lyase
(Elsevier, 1987-12)
Three bacterial strains, Agrobacterium radiobacter, Klebsiella oxytoca, and Kluyvera ascorbata, isolated by enrichment culture for carbon to phosphorus bond cleavage ability, were analyzed for the mode of C---P bond fission. ...
Evaluating hydrogen bond interactions in enzymes containing Mn(III)-histidine complexation using manganese-imidazole complexes
(Springer-Verlag; SBIC, 2003-02)
It is often difficult to control hydrogen bond interactions in small molecule compounds that model metalloenzyme active sites. The imidazole-containing ligands 4,5-dicarboxyimidazole (H 3 DCBI) and 4,5-dicarboxy- N ...
Mutagenicity in Salmonella assays of cyclohexane epoxide derivatives
(Elsevier, 1985-06)
15 Cyclohexane epoxide derivatives were synthesized and compared for direct mutagenicity an bacterial toxicity using Salmonella typhimurium strain TA100 in the liquid suspension and spot-test version of the Ames procedure. ...
Lectin-based homogeneous enzyme-linked binding assay for estimating the type and relative amount of carbohydrate within intact glycoproteins
(Elsevier, 1992-04)
The feasibility of using a new lectin-based homogeneous enzyme-linked binding assay for estimating the type and relative amount of specific carbohydrate structures within intact glycoproteins is examined. Malate ...