Now showing items 11-20 of 21
Classifications of ovarian cancer tissues by proteomic patterns
(WILEY-VCH Verlag, 2006-11)
Ovarian cancer is a morphologically and biologically heterogeneous disease. The identification of type-specific protein markers for ovarian cancer would provide the basis for more tailored treatments, as well as clues for ...
Selective resonance enhanced multiphoton ionization of aromatic polymers in supersonic beam mass spectrometry
(Elsevier, 1991-07-15)
Various polymers have been studied using a pulsed laser evaporation method for volatilizing these species and entraining them into a supersonic jet expansion followed by resonance enhanced multiphoton ionization (REMPI) ...
Resonance enhanced multiphoton ionization of nucleosides by using pulsed-laser desorption in supersonic beam mass spectrometry
(Elsevier, 1989-04-01)
Pulsed-laser desorption is used as a means of volatilizing nucleosides into the gas phase for entrainment into a supersonic jet expansion. These species are then studied in a time-of-flight mass spectrometer by resonance ...
Toward high sequence coverage of proteins in human breast cancer cells using on-line monolith-based HPLC-ESI-TOF MS compared to CE MS
(WILEY-VCH Verlag, 2006-06)
A method is developed toward high sequence coverage of proteins isolated from human breast cancer MCF10 cell lines using a 2-D liquid separations. Monolithic-capillary columns prepared by copolymerizing styrene with ...
Validation of reverse phase protein array for practical screening of potential biomarkers in serum and plasma: Accurate detection of CA19-9 levels in pancreatic cancer
(WILEY-VCH Verlag, 2008-08)
The current study analyzed reverse phase protein arrays (RPPA) as a means to experimentally validate biomarkers in blood samples. One microliter samples of sera ( n 14= 1471), and plasma ( n 14= 1478) were serially diluted ...
Comprehensive analysis of proteins of pH fractionated samples using monolithic LC/MS/MS, intact MW measurement and MALDI-QIT-TOF MS
(John Wiley & Sons, Ltd., 2007-03)
A comprehensive platform that integrates information from the protein and peptide levels by combining various MS techniques has been employed for the analysis of proteins in fully malignant human breast cancer cells. The ...
A proteomic analysis of Psychrobacter articus 273-4 adaptation to low temperature and salinity using a 2-D liquid mapping approach
(WILEY-VCH Verlag, 2007-02)
Psychrobacter 273-4 was isolated from a 20 000–40 000–year-old Siberian permafrost core, which is characterized by low temperature, low water activity, and high salinity. To explore how 273-4 survives in the permafrost ...
Galanin inhibits rat pancreatic amylase release via cholinergic suppression
(Elsevier, 1992)
The effects of galanin on pancreatic exocrine function were examined using rat pancreatic tissues. In anesthetized rats, galanin (40 [mu]g/kg/h) decreased amylase secretion stimulated by 2-deoxy glucose (5.8 +/- 0.1 vs. ...
Identification of low molecular weight proteins isolated by 2-D liquid separations
(John Wiley & Sons, Ltd., 2004-07)
Proteins with molecular mass ( M r ) <20 kDa are often poorly separated in 2-D sodium dodecyl sulfate polyacrylamide gel electrophoresis. In addition, low- M r proteins may not be readily identified using peptide mass ...
Automated integration of monolith-based protein separation with on-plate digestion for mass spectrometric analysis of esophageal adenocarcinoma human epithelial samples
(WILEY-VCH Verlag, 2006-09)
A unique approach of automating the integration of monolithic capillary HPLC-based protein separation and on-plate digestion for subsequent MALDI-MS analysis has been developed. All liquid-handling procedures were performed ...