Endogenous overexpression of Populus MYB186 increases trichome density, improves insect pest resistance, and impacts plant growth
dc.contributor.author | Plett, Jonathan M. | en_US |
dc.contributor.author | Wilkins, Olivia | en_US |
dc.contributor.author | Campbell, Malcolm M. | en_US |
dc.contributor.author | Ralph, Steven G. | en_US |
dc.contributor.author | Regan, Sharon | en_US |
dc.date.accessioned | 2011-01-31T17:43:25Z | |
dc.date.available | 2012-01-03T20:18:47Z | en_US |
dc.date.issued | 2010-11 | en_US |
dc.identifier.citation | Plett, Jonathan M.; Wilkins, Olivia; Campbell, Malcolm M.; Ralph, Steven G.; Regan, Sharon; (2010). "Endogenous overexpression of Populus MYB186 increases trichome density, improves insect pest resistance, and impacts plant growth." The Plant Journal 64(3): 419-432. <http://hdl.handle.net/2027.42/79232> | en_US |
dc.identifier.issn | 0960-7412 | en_US |
dc.identifier.issn | 1365-313X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/79232 | |
dc.description.abstract | Trichomes are specialized epidermal cells that generally play a role in reducing transpiration and act as a deterrent to herbivory. In a screen of activation-tagged Populus tremula × Populus alba 717-1B4 trees, we identified a mutant line, fuzzy , with increased foliar trichome density. This mutant also had a 35% increase in growth rate and a 200% increase in the rate of photosynthesis as compared with wild-type poplar. The fuzzy mutant had significant resistance to feeding by larvae of the white-spotted tussock moth ( Orgyia leucostigma ), a generalist insect pest of poplar trees. The fuzzy trichome phenotype is attributable to activation tagging and increased expression of the gene encoding PtaMYB186 , which is related to Arabidopsis thaliana MYB106 , a known regulator of trichome initiation. The fuzzy phenotype can be recapitulated by overexpressing PtaMYB186 in poplar. PtaMYB186 overexpression results in reconfiguration of the poplar transcriptome, with changes in the transcript abundance of suites of genes that are related to trichome differentiation. It is notable that a plant with misexpression of a gene responsible for trichome development also had altered traits related to growth rate and pest resistance, suggesting that non-intuitive facets of plant development might be useful targets for plant improvement. | en_US |
dc.format.extent | 708478 bytes | |
dc.format.extent | 790677 bytes | |
dc.format.extent | 31893 bytes | |
dc.format.extent | 3106 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.subject.other | Poplar | en_US |
dc.subject.other | Activation Tagging | en_US |
dc.subject.other | R2R3-MYB | en_US |
dc.subject.other | Trichome | en_US |
dc.subject.other | Herbivory | en_US |
dc.subject.other | Growth Rate | en_US |
dc.title | Endogenous overexpression of Populus MYB186 increases trichome density, improves insect pest resistance, and impacts plant growth | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationother | Department of Biology, Queen’s University, Kingston, ON K7L 3N6, Canada | en_US |
dc.contributor.affiliationother | Department of Cell & Systems Biology, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada | en_US |
dc.contributor.affiliationother | Department of Biology, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada | en_US |
dc.contributor.affiliationother | Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks Street, Toronto, ON M5S 3B2, Canada | en_US |
dc.contributor.affiliationother | Department of Biology, University of North Dakota, Grand Forks, ND 58202-9019, USA | en_US |
dc.identifier.pmid | 20807210 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/79232/1/TPJ_4343_sm_FigsS1-S3.pdf | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/79232/2/j.1365-313X.2010.04343.x.pdf | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/79232/3/TPJ_4343_sm_TableS5.pdf | |
dc.identifier.doi | 10.1111/j.1365-313X.2010.04343.x | en_US |
dc.identifier.source | The Plant Journal | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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