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Patterned Hydrogel Substrates for Cell Culture with Electrohydrodynamic Jet Printing

dc.contributor.authorPoellmann, Michael J.en_US
dc.contributor.authorBarton, Kira L.en_US
dc.contributor.authorMishra, Sandipanen_US
dc.contributor.authorJohnson, Amy J. Wagoneren_US
dc.date.accessioned2011-11-10T15:30:59Z
dc.date.available2012-11-02T18:56:20Zen_US
dc.date.issued2011-09-09en_US
dc.identifier.citationPoellmann, Michael J.; Barton, Kira L.; Mishra, Sandipan; Johnson, Amy J. Wagoner (2011). "Patterned Hydrogel Substrates for Cell Culture with Electrohydrodynamic Jet Printing." Macromolecular Bioscience 11(9): 1164-1168. <http://hdl.handle.net/2027.42/86801>en_US
dc.identifier.issn1616-5187en_US
dc.identifier.issn1616-5195en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/86801
dc.description.abstractCells respond to and are directed by physiochemical cues in their microenvironment, including geometry and substrate stiffness. The development of substrates for cell culture with precisely controlled physiochemical characteristics has the potential to advance the understanding of cell biology considerably. In this communication, E‐jet printing is introduced as a method for creating high‐resolution protein patterns on substrates with controlled elasticity. It is the first application of E‐jet printing on a soft surface. Protein spots as small as 5 µm in diameter on polyacrylamide are demonstrated. The patterned hydrogels are shown to support cell attachment and spreading. Polyacrylamide substrates patterned by E‐jet printing may be applied to further the study of cellular mechanobiology. Electrohydrodynamic jet (E‐jet) printing is used to print proteins on soft, polyacrylamide substrates with micrometer‐scale resolution. Immunofluorescence confirms the presence of patterned cell adhesion proteins, and the patterned hydrogels are shown to support cell attachment and spreading. The method can be used to create cell substrates with well‐defined protein geometries and elastic properties.en_US
dc.publisherWILEY‐VCH Verlagen_US
dc.subject.otherElectrohydrodynamic Jeten_US
dc.subject.otherPolyacrylamideen_US
dc.subject.otherHydrogelsen_US
dc.subject.otherProteinsen_US
dc.subject.otherSurface Modificationen_US
dc.titlePatterned Hydrogel Substrates for Cell Culture with Electrohydrodynamic Jet Printingen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelMaterials Science and Engineeringen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Mechanical Engineering, University of Michigan, 2350 Hayward St., Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationotherDepartment of Mechanical Science and Engineering, University of Illinois at Urbana‐Champaign, 1206 W. Green St., Urbana, IL 61801, USAen_US
dc.contributor.affiliationotherDepartment of Mechanical, Aerospace, and Nuclear Engineering, Rensselaer Polytechnic Institute, 110 8 th St., Troy, NY 12180, USAen_US
dc.contributor.affiliationotherDepartment of Bioengineering, University of Illinois at Urbana‐Champaign, 1304 W. Springfield Ave., Urbana, IL 61801, USAen_US
dc.contributor.affiliationotherDepartment of Mechanical Science and Engineering, University of Illinois at Urbana‐Champaign, 1206 W. Green St., Urbana, IL 61801, USA.en_US
dc.identifier.pmid21656685en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/86801/1/1164_ftp.pdf
dc.identifier.doi10.1002/mabi.201100004en_US
dc.identifier.sourceMacromolecular Bioscienceen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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