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Anonymous and EST-based microsatellite DNA markers that transfer broadly across the fig genus (Ficus, Moraceae)

dc.contributor.authorHeer, Katrin
dc.contributor.authorMachado, Carlos A.
dc.contributor.authorHimler, Anna G.
dc.contributor.authorHerre, E. Allen
dc.contributor.authorKalko, Elisabeth K. V.
dc.contributor.authorDick, Christopher W.
dc.date.accessioned2012-08-19T11:47:36Z
dc.date.available2012-08-19T11:47:36Z
dc.date.issued2012
dc.identifier.citationAmerican Journal of Botany Primer Notes & Protocols in the Plant Sciences, 2012, e330–e333 <http://hdl.handle.net/2027.42/92471>en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/92471
dc.description.abstract• Premise of the study: We developed a set of microsatellite markers for broad utility across the species-rich pantropical tree genus Ficus (fig trees). The markers were developed to study population structure, hybridization, and gene flow in neotropical species. • Methods and Results: We developed seven novel primer sets from expressed sequence tag (EST) libraries of F. citrifolia and F. popenoei (subgen. Urostigma sect. Americana) and optimized five previously developed anonymous loci for cross-species amplification. The markers were successfully tested on four species from the basal subgenus Pharmacosycea sect. Pharmaco- sycea (F. insipida, F. maxima, F. tonduzii, and F. yoponensis) and seven species of the derived subgenus Urostigma (F. citrifolia, F. colubrinae, F. costaricana, F. nymphaeifolia, F. obtusifolia, F. pertusa, and F. popenoei). The 12 markers amplified consis- tently and displayed polymorphism in all the species. • Conclusions: This set of microsatellite markers is transferable across the phylogenetic breadth of Ficus, and should therefore be useful for studies of population structure and gene flow in approximately 750 fig species worldwide.en_US
dc.language.isoen_USen_US
dc.titleAnonymous and EST-based microsatellite DNA markers that transfer broadly across the fig genus (Ficus, Moraceae)en_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biology
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumEcology and Evolutionary Biology, Department of (EEB)en_US
dc.contributor.affiliationumDepartment of Biology, University of Marylanden_US
dc.contributor.affiliationotherDepartment of Biology, University of Maryland, College Park, Maryland 20742 USAen_US
dc.contributor.affiliationotherDepartment of Entomology, University of Arizona, Tucson, Arizona 85721 USAen_US
dc.contributor.affiliationotherSmithsonian Tropical Research Institute, Balboa, Panamaen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/92471/1/Heer2012.pdf
dc.identifier.doidoi:10.3732/ajb.1200032
dc.identifier.sourceAmerican Journal of Botany Primer Notes & Protocols in the Plant Sciencesen_US
dc.description.mapping85en_US
dc.owningcollnameEcology and Evolutionary Biology, Department of (EEB)


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