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Detection of disordered regions in globular proteins using 13 C‐detected NMR

dc.contributor.authorGray, Felicia L. V.en_US
dc.contributor.authorMurai, Marcelo J.en_US
dc.contributor.authorGrembecka, Jolantaen_US
dc.contributor.authorCierpicki, Tomaszen_US
dc.date.accessioned2012-12-11T17:37:19Z
dc.date.available2014-02-03T16:21:44Zen_US
dc.date.issued2012-12en_US
dc.identifier.citationGray, Felicia L. V.; Murai, Marcelo J.; Grembecka, Jolanta; Cierpicki, Tomasz (2012). "Detection of disordered regions in globular proteins using 13 C‐detected NMR." Protein Science 21(12): 1954-1960. <http://hdl.handle.net/2027.42/94460>en_US
dc.identifier.issn0961-8368en_US
dc.identifier.issn1469-896Xen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/94460
dc.description.abstractCharacterization of disordered regions in globular proteins constitutes a significant challenge. Here, we report an approach based on 13 C‐detected nuclear magnetic resonance experiments for the identification and assignment of disordered regions in large proteins. Using this method, we demonstrate that disordered fragments can be accurately identified in two homologs of menin, a globular protein with a molecular weight over 50 kDa. Our work provides an efficient way to characterize disordered fragments in globular proteins for structural biology applications.en_US
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherProtein Disorderen_US
dc.subject.otherStructural Biologyen_US
dc.subject.otherNMR Spectroscopyen_US
dc.subject.otherMeninen_US
dc.titleDetection of disordered regions in globular proteins using 13 C‐detected NMRen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pathology, University of Michigan, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumProgram in Chemical Biology, University of Michigan, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumDepartment of Pathology, University of Michigan, 1150 W. Medical Center Drive, MSRB1 4516, Ann Arbor, MI 48109en_US
dc.identifier.pmid23047544en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/94460/1/2174_ftp.pdf
dc.identifier.doi10.1002/pro.2174en_US
dc.identifier.sourceProtein Scienceen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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