View Item 

Show simple item record

The use of filtration techniques for the lysis and study of red blood cells

dc.contributor.authorHultquist, Donald E.en_US
dc.contributor.authorVasko, Michael R.en_US
dc.contributor.authorGray, Robert H.en_US
dc.date.accessioned2013-06-18T18:32:33Z
dc.date.available2013-06-18T18:32:33Z
dc.date.issued1977en_US
dc.identifier.citationHultquist, Donald E.; Vasko, Michael R.; Gray, Robert H. (1977). "The use of filtration techniques for the lysis and study of red blood cells." American Journal of Hematology 3(4): 237-244. <http://hdl.handle.net/2027.42/98218>en_US
dc.identifier.issn0361-8609en_US
dc.identifier.issn1096-8652en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/98218
dc.description.abstractA filtration technique for the gentle lysis of erythrocytes has been developed using cellulose triacetate membranes. When cell suspensions are filtered under nitrogen pressure, lysis occurs at the surface of the filter in such a way that the cell ghosts are retained on the filter. The contents of the cell are extruded through the pores of the filter without mixing with the cell suspension. Cell ghosts and intact erythrocytes have been collected on membranes and examined by electron microscopy. These preparations have the advantage of being free of the structural artifacts that result from centrifugation. In addition, the filter facilitates preparation for electron microscopy by providing a support for the sample during fixation and then dissolving during the dehydration of the sample.en_US
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherHemolysisen_US
dc.subject.otherElectron Microscopyen_US
dc.subject.otherErythrocytesen_US
dc.subject.otherFiltrationen_US
dc.subject.otherCell Membraneen_US
dc.titleThe use of filtration techniques for the lysis and study of red blood cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelOncology and Hematologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, The University of Michigan, Ann Arboren_US
dc.contributor.affiliationumDepartment of Biological Chemistry Medical Science Bldg. I, The University of Michigan, Ann Arbor, MI 48109en_US
dc.contributor.affiliationumDepartment of Environmental and Industrial Health, The University of Michigan, Ann Arboren_US
dc.identifier.pmid602939en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/98218/1/2830030401_ftp.pdf
dc.identifier.doi10.1002/ajh.2830030401en_US
dc.identifier.sourceAmerican Journal of Hematologyen_US
dc.identifier.citedreferenceWeed RI, LaCelle PL, Merrill EW: Metabolic dependence of red cell deformability. J Clin Invest 48: 795 – 809. 1969.en_US
dc.identifier.citedreferenceCard RT, Weintraub LR: Metabolic abnormalities of erythrocytes in severe iron deficiency. Blood 37: 727 – 732, 1971.en_US
dc.identifier.citedreferenceFrasca JM, Parks VR: A routine technique for double‐staining ultrathin sections using uranyl and lead salts. J Cell Biol 25: 157 – 161, 1965.en_US
dc.identifier.citedreferenceReynolds ES: The use of lead citrate at high pH as an electron‐opaque stain in electron microscopy. J Cell Biol 17: 208 – 215, 1963.en_US
dc.identifier.citedreferenceBeams HW, Kessel RG: Electron microscope and ultracentrifugation studies on the rat reticulocyte. Am J Anat 118: 471 – 507, 1966.en_US
dc.identifier.citedreferenceBeams HW, Kessel RG: The effects of ultrafiltration on the mammalian erythrocyte. Anat Rec 115: 541 – 550, 1966.en_US
dc.identifier.citedreferenceBaudhuin P, Evrard P, Berthet J: Electron microscopic examination of subcellular fraction. I. The preparation of representative samples from suspensions of particles. J Cell Biol 32: 181 – 191, 1967.en_US
dc.identifier.citedreferenceJandl JH, Simmons RL, Castle WB: Red cell filtration and the pathogenesis of certain hemolytic anemias. Blood 18: 133 – 148, 1961.en_US
dc.identifier.citedreferenceProthero JW, Burton AC: The physics of blood flow in capillaries. III. The pressure required to deform erythrocytes in acid‐citratedextrose. Biophys J 2: 213 – 222, 1962.en_US
dc.identifier.citedreferenceGregersen ML, Bryant CA, Mannerle WE, Usami S, Chien S: Flow characteristics of human erythrocytes through polycarbonate sieves. Science 157: 825 – 827, 1967.en_US
dc.identifier.citedreferenceReynaud AJ, King EB: A new filter for diagnostic cytology. Acta Cytol 11: 289 – 294, 1967.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
2830030401_ftp.pdf
Size:
992.1KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.