Reconstructed CT slices for Cranium of Castoroides (University of Michigan Museum of Paleontology catalog number UMMP VP 3110) as a series of TIFF images. Raw projections are not included in this dataset. The reconstructed slice data from the scan are offered here as a series of unsigned 16-bit integer TIFF images. The upper left corner of the first image (*_0000.tif) is the XYZ origin.
Reconstructed CT slices for Cranium of Castoroides (University of Michigan Museum of Paleontology catalog number UMMP VP 3110) as a series of TIFF images. Raw projections are not included in this dataset. The reconstructed slice data from the scan are offered here as a series of unsigned 16-bit integer TIFF images. The upper left corner of the first image (*_0000.tif) is the XYZ origin.
The internet has significantly transformed how news is produced, consumed, and distributed. As a result, the news
industry has transitioned from ad-supported to subscription-based models regulated by digital paywalls. In light of this
disruption, it’s crucial to investigate not only how news consumers adapt to this change but also how economic incentives
shape content coverage. We analyzed the staggered adoption of digital paywalls by 17 regional U.S. newspapers over 17
years in a difference-in-difference framework to examine the impact of paywall adoption on topical news content coverage.
Our results reveal a small but significant decrease in local and soft news coverage, with varying effects across different
urban contexts. Specifically, local news coverage experienced a more substantial decline in smaller cities (population <
500,000) and regions experiencing an influx of younger residents (age < 40 years). Conversely, soft news coverage increased
in areas with a younger demographic influx, indicating a strategic shift by newspapers to cater to digital-savvy audiences
and adapt to changing consumption patterns. Our findings underscore the delicate balance between financial imperatives
and editorial choices in the newspaper industry and highlight the need for ongoing research into the effects of digital
monetization strategies on journalistic content creation, media plurality, and civic accountability.
We took advantage of the randomized allocation of the US EPA's funding for school bus replacements and retrofits to causally assess the impacts of upgrading buses through the EPA’s national School Bus Rebate Program on various outcomes including attendance, educational performance, and community air quality (PM2.5). Specifically, we used classical intent-to-treat analyses for randomized controlled trials to compare the changes in school district average attendance, test scores (reading language arts and math), and PM2.5 levels after vs before the 2012 through 2017 lotteries by funding selection status. We used overall district attendance rates and average standardized test scores since rates were not available for only school-bus riders.
The survival and beta models forecasting ice cover around the Apostle Island National Lakeshore use Lake Superior surface water temperature data collected from the Great Lakes Environmental Research Laboratory (GLERL). GLERL hosts Lake Superior surface water temperature data pre-1995 from the Large Lake Thermodynamics Model (LLTM) and post-1995 from the Great Lakes Surface Environmental Analysis (GLSEA). The pre-1995 data is currently being moved and is unavailable from GLERL, and therefore, the (entire) surface water temperature record used by the models is hosted here. Please see the methods for direct data access links.
The LLTM, the source of the pre-1995 data, is described in Croley II, T. E., & Assel, R. A. (1994). A one-dimensional ice thermodynamics model for the Laurentian Great Lakes. Water Resources Research, 30(3), 625–639. Documentation on GLSEA can be found on their website.
The data sources and methods used to process the raw data are described in the paper forthcoming in Science and the associated Supplementary Information. A preprint for an earlier version of this paper is available here: https://osf.io/preprints/socarxiv/754e3. These data are anonymized (see Methodology for details). Consequently, running the same code on these data vs. the data in the paper does not yield *identical* results but qualitatively similar ones.
J. M. Z. Dumlao, M. Teplitskiy, Science, forthcoming. and Zumel Dumlao, J. M. and M. Teplitskiy. 2023. “The Effect of Reviewer Geographical Diversity on Evaluations Is Reduced by Anonymizing Submissions”. Retrieved (osf.io/preprints/socarxiv/754e3).
LSD1 histone H3K4 demethylase and its binding partner PHF21A, a reader protein for unmethylated H3K4, both undergo neuron-specific microexon splicing. The LSD1 neuronal microexon weakens H3K4 demethylation activity and can alter the substrate specificity to H3K9 or H4K20. Meanwhile, the PHF21A neuronal microexon interferes with nucleosome binding. However, the temporal expression patterns of LSD1 and PHF21A splicing isoforms during brain development remain unknown. In this work, we report that neuronal PHF21A isoform expression precedes neuronal LSD1 isoform expression during human neuron differentiation and mouse brain development. The asynchronous splicing events resulted in stepwise deactivation of the LSD1-PHF21A complex in reversing H3K4 methylation. We further show that the enzymatically inactive LSD1-PHF21A complex interacts with neuron-specific binding partners, including MYT1-family transcription factors and post-transcriptional mRNA processing proteins such as VIRMA. The interaction with the neuron-specific components, however, did not require the PHF21A microexon, indicating that the neuronal proteomic milieu, rather than the microexon-encoded PHF21A segment, is responsible for neuron-specific complex formation. These results indicate that the PHF21A microexon is dispensable for neuron-specific protein-protein interactions, yet the enzymatically inactive LSD1-PHF21A complex might have unique gene-regulatory roles in neurons.
Citation to related publication:
Nagai, M., Porter, R. S., Miyasato, M., Wang, A., Gavilan, C. M., Hughes, E. D., Wu, M. C., Saunders, T. L., Iwase, S. Neuronal splicing of the unmethylated histone H3K4 reader, PHF21A, prevents excessive synaptogenesis. Journal of Biological Chemistry (2024).
In this study, we took advantage of the randomized allocation of the US EPA's funding for school bus replacements and retrofits to causally assess the impacts of upgrading buses on student attendance through the EPA’s national School Bus Rebate Program. Specifically, we used classical intent-to-treat analyses for randomized controlled trials to compare the change in school district level attendance rates after vs before the 2012 through 2017 lotteries by funding selection status . We used overall district attendance rates since rates were not available for only school-bus riders.
Pedde, M., Szpiro, A., Hirth, R. et al. Randomized design evidence of the attendance benefits of the EPA School Bus Rebate Program. Nat Sustain (2023). https://doi.org/10.1038/s41893-023-01088-7
In this study, we took advantage of the randomized allocation of the US EPA's funding for school bus replacements and retrofits to causally assess the impacts of upgrading buses on students' educational performance through the EPA’s national School Bus Rebate Program. Specifically, we used classical intent-to-treat analyses for randomized controlled trials to compare the change in school district level reading and language arts and math standardized test scores after vs before the 2012 through 2016 lotteries by funding selection status . We used overall district average standardized test scores since rates were not available for only school-bus riders.
