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The research described here includes a combination of in vivo animal studies and in vitro cell studies. The animal studies were conducted in NSG mice purchased from Jackson Laboratories and involve implantation of a cell-laden scaffold, animal monitoring, and scaffold explantation. After explantatio...  [more]

The research described here includes a combination of in vivo animal studies and in vitro cell studies. The animal studies were conducted in NSG mice purchased from Jackson Laboratories and involve implantation of a cell-laden scaffold, animal monitoring, and scaffold explantation. After explantation, scaffolds could be analyzed using PCR or staining. The in vitro cell studies involved administration of Exendin-4 during differentiation of hPSC-derived beta cells and had endpoints such as PCR, flow cytometry, and staining.  [less]

The research described here includes a combination of in vivo animal studies and in vitro cell studies. The animal studies were conducted in NSG mice purchased from Jackson Laboratories and involve implantation of a cell-laden scaffold, animal monitoring, and scaffold explantation. After explantatio...  [more]

The research described here includes a combination of in vivo animal studies and in vitro cell studies. The animal studies were conducted in NSG mice purchased from Jackson Laboratories and involve implantation of a cell-laden scaffold, animal monitoring, and scaffold explantation. After explantation, scaffolds could be analyzed using PCR or staining. The in vitro cell studies involved administration of Exendin-4 during differentiation of hPSC-derived beta cells and had endpoints such as PCR, flow cytometry, and staining.  [less]

Cover crops support ecosystem services in agroecosystems, but their performance can be highly variable. Functional trait ecology provides a useful framework for understanding variation in cover crop performance across different growing conditions. However, trait variation within species remains unde...  [more]

Cover crops support ecosystem services in agroecosystems, but their performance can be highly variable. Functional trait ecology provides a useful framework for understanding variation in cover crop performance across different growing conditions. However, trait variation within species remains understudied compared to variation between species. In a two-year experiment, we measured nine functional traits for three cover crop species across 13 fields on working farms that spanned a gradient of soil health. Each field contained three cover crop treatments: a functionally diverse mixture of cereal rye (Secale cereale), crimson clover (Trifolium incarnatum), and dwarf-essex rapeseed (Brassica napus), and rye and clover monocrops. We evaluated i) the magnitude and relative importance of intraspecific and interspecific trait variation; ii) which soil health indicators best explained trait variation; and iii) whether interspecific interactions in mixture induced trait plasticity. Despite strong trait contrasts between species, intraspecific trait variation comprised 50% of total trait variation, on average. Trait variation was best explained by particulate organic matter nitrogen (POM N), soil phosphorus, pH, and permanganate oxidizable carbon for clover; by POM N and soil phosphorus for rye; and by POM N for dwarf essex. Rye and clover also showed significant trait plasticity in mixture relative to monocrop treatments. Our study demonstrates that intraspecific and interspecific trait variation are equally important, and that examining trait variation within species can improve the ability to predict cover crop outcomes. This information can inform cropping system design in distinct contexts to promote success of component species and complementary ecosystem functions.   [less]

This dataset contains results from the BATS-R-US multispecies MHD code simulating the solar wind interaction at Mars at the following orbital and solar cycle locations: solar maximum at perihelion, solar maximum at aphelion, solar minimum at perihelion, solar minimum at aphelion. These simulations c...  [more]

This dataset contains results from the BATS-R-US multispecies MHD code simulating the solar wind interaction at Mars at the following orbital and solar cycle locations: solar maximum at perihelion, solar maximum at aphelion, solar minimum at perihelion, solar minimum at aphelion. These simulations contain results with and without the crustal magnetic fields and includes variables such as the magnetic field and ion density., 2025-02-25: The metadata in this dataset has been updated in response to reviewer comments during the journal review process. Additional Tecplot 360 EX file containing MAVEN multifluid MHD simulation data added. The multifluid MHD simulation result from this result is from the MAVEN simulation library. The simulation result is run for 25,000 iterations., and 2025-04-28: The following files have been replaced with the following as these files contain the nominal solar wind conditions: z_0_mhd_permax.plt -> permax_3d.plt z_0_mhd_aphmax.plt -> aphmax_3d.plt z_0_mhd_permin.plt -> permin_3d.plt z_0_mhd_aphmin.plt -> aphmin_3d.plt The following file has been removed as the slice can be extracted from permax_3d.plt: x_0_mhd_permax.plt  [less]

We analyzed satisfaction with care, out-of-pocket costs, and specialist access among community-dwelling Medicare Current Beneficiary Survey respondents, 2015–2019, in the 50 states and Washington, DC. For each measure, we constructed a binary indicator indicating very satisfied (vs. very dissatisfie...  [more]

We analyzed satisfaction with care, out-of-pocket costs, and specialist access among community-dwelling Medicare Current Beneficiary Survey respondents, 2015–2019, in the 50 states and Washington, DC. For each measure, we constructed a binary indicator indicating very satisfied (vs. very dissatisfied to satisfied)., We used logistic regression to model outcomes as a function of Medicare Advantage - MA (vs. Traditional Medicare - TM) enrollment, respondent-reported race/ethnicity, and interactions of MA with race/ethnicity. Race/ethnicity was categorized as non-Hispanic Black, Hispanic, and non-Hispanic White. We adjusted for age, sex, education, income, tobacco use, chronic conditions, functional limitations, disability, and geographic factors. Racial/ethnic disparities reflect effects of structural factors that systematically disadvantage members of minoritized racial/ethnic groups. Because structural racism contributes to disparities in socioeconomic status (including income and education), we verified that our estimates did not change appreciably when we did not adjust for socioeconomic factors. , and Analyses were weighted by a composite of survey weights and propensity score weights to balance MA and TM populations within racial/ethnic groups. Separate analyses were conducted for beneficiaries with vs. without dual eligibility for full Medicaid. We used SAS to process the data.  [less]

Studies of fate patterning during development typically emphasize cell-cell communication via diffusible chemical signals. Recent experiments on stem cell colonies (see Xue et al. Nature Materials 2018), however, suggest that in some cases mechanical stresses, rather than secreted chemicals, enable ...  [more]

Studies of fate patterning during development typically emphasize cell-cell communication via diffusible chemical signals. Recent experiments on stem cell colonies (see Xue et al. Nature Materials 2018), however, suggest that in some cases mechanical stresses, rather than secreted chemicals, enable long-ranged cell-cell interactions that specify positional information and pattern cell fates. The authors of this earlier publication reported a set of in vitro experiments in which uniformly supplied chemical media induced spatially patterned fates in cell colony in a disc geometry. They provided significant evidence that inter-cellular mechanical interactions, as well as mechanical interactions between cells and the substrate, play an important role in this in vitro differentiation process. As part of these experiments, they showed that the concentric width of the outer fate domain is approximately constant as the colony diameter is increased from 300 um to 800 um. In this subsequent publication, we propose a mathematical model for this fate patterning process and explore how the fate pattern depends on substrate stiffness. The experimental images of cell colonies, both for varying cell colony diameter (from Xue et al. Nature Materials 2018) and for varying substrate stiffness (data generated for the publication linked to these data), are provided here. Each example has an image for PAX3 signal (marker for outer fate domain; Paired box gene 3) and an image for DAPI signal (staining nuclei; 4′,6-diamidino-2-phenylindole).  [less]

In a previous study (Xue et al. Nature Materials 2018), the authors showed that a key fate patterning event in vertebrate development can be reproduced in an in vitro stem cell culture. They further showed that this in vitro fate pattern seems to depend on mechanical signals rather than secreted che...  [more]

In a previous study (Xue et al. Nature Materials 2018), the authors showed that a key fate patterning event in vertebrate development can be reproduced in an in vitro stem cell culture. They further showed that this in vitro fate pattern seems to depend on mechanical signals rather than secreted chemical signals. In this follow-up study, a mathematical model of this process is proposed. The code in this deposit is for the simulation of this mathematical model in various cell layer geometries and substrate geometries. These geometries include a 1D cell layer, quasi-1D stripe geometry, disc geometry (all on a very thin substrate or a substrate composed of microposts) as well as a 1D cell layer on a finite-thickness substrate. Our model implies that the width of the outer fate domain varies non-monotonically with substrate stiffness, a prediction that we confirm experimentally.  [less]

This dataset contains full results tables for differentially methylated region analysis in the paper "Developmental Programming: Differing impact of prenatal testosterone and prenatal bisphenol-A -treatment on hepatic methylome in female sheep". Each table contains the following columns: chromosome ...  [more]

This dataset contains full results tables for differentially methylated region analysis in the paper "Developmental Programming: Differing impact of prenatal testosterone and prenatal bisphenol-A -treatment on hepatic methylome in female sheep". Each table contains the following columns: chromosome (chr), start genomic position of region (start), end genomic position of region (end), width of region (width), number of CpGs in region (nCpG), test statistic (stat), p-value (pval), FDR corrected p-value (qval), gene located within or overlapping region (gene), mean difference between comparison groups (meanDiff). Supplemental Table 1: Differentially methylated regions associated with prenatal-Testosterone treatment in sheep liver Supplemental Table 3: Differentially methylated regions associated with prenatal-BPA treatment in sheep liver Supplemental Table 6: Differentially methylated regions in Controls between the prenatal-Testosterone and prenatal-BPA treated sheep.  [less]

Five short videos, three about food preparation, one about agriculture, and one about catching and cooking a giant pouched rat (Cricetomys)., The videos were incidental byproducts of Heath's linguistic fieldwork on the endangered Pere language spoken in Bonosso village on the outskirts of Tiéningbou...  [more]

Five short videos, three about food preparation, one about agriculture, and one about catching and cooking a giant pouched rat (Cricetomys)., The videos were incidental byproducts of Heath's linguistic fieldwork on the endangered Pere language spoken in Bonosso village on the outskirts of Tiéningboué city in north central Côte d'Ivoire. The footage was shot by project manager Minkailou Djiguiba as indicated in the credits at the end of each video. , and A much larger number of videos have been made from Mali and Burkina Faso and are deposited in other collections in Deep Blue Data. Many of the videos from all of these countries are also disseminated in a youtube channel that is managed by Djiguiba:  https://www.youtube.com/@PratiquesCulturellesMaliBF. For more on Pere language see the Deep Blue Dataset "Pere language lexical datasheets and audio files" ( https://doi.org/10.7302/k6r9-r160) and the grammar cited as “A grammar of Pere (Bere, Mbre) of Côte d'Ivoire“ Under Related items in Deep Blue Documents, below ( https://hdl.handle.net/2027.42/163773) (copy at Zenodo,  https://zenodo.org/records/3354193).   [less]

Research Overview: This dataset captures the longitudinal experience of both patients undergoing hematopoietic cell transplantation (HCT) and their caregivers through continuous physiological monitoring, daily mood reporting, and periodic health assessments. The data provides unprecedented insight i...  [more]

Research Overview: This dataset captures the longitudinal experience of both patients undergoing hematopoietic cell transplantation (HCT) and their caregivers through continuous physiological monitoring, daily mood reporting, and periodic health assessments. The data provides unprecedented insight into the dynamic relationship between caregiver well-being and patient outcomes during the critical post-transplant period. and Key Points: Digital biomarker data collected from 166 HCT caregiver-patient dyads over 120 days post-transplant Comprehensive physiological monitoring through wearable devices (Fitbit® Charge 3) Integration of daily mood reports, PROMIS® health measures, and clinical outcomes Unique paired data structure allowing analysis of caregiver-patient dynamics  [less]